‘다유들깨’품종의 하배축에서 캘러스를 통한 고효율 식물재분화
Efficient plant regeneration through callus induction from the hypocotyl of Perilla frutescens L var. Dayu
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This study was conducted to establish an efficient plant regeneration system in ‘Dayu’, a Korean variety of Perilla frutescens developed for seed oil production, in conjunction with the previously studied variety ‘Namcheon’. The healthiest callus was formed on the hypocotyl explants cultured on a medium containing 0.1 mg/L NAA and 0.5 mg/L BA, outperforming the leaf and cotyledon samples. In both dark and long-day conditions, Dayu consistently exhibited significantly higher shoot regeneration rates compared with Namcheon. The highest shoot regeneration rates in Dayu were observed from the hypocotyl explants cultured on 0.1 mg/L NAA and 0.5 mg/L BA media, with shoot regeneration rates of 84.4% and 86.7% under dark and long-day conditions, respectively. Various combinations of plant growth regulators were tested to establish the optimal shoot regeneration conditions for Dayu hypocotyl explants. The results demonstrated that the highest shoot regeneration rate (90%) was achieved when 0.5 mg/L of BA was added to the medium without NAA. Among the regenerated shoots, 70.5% were normal plants, while 19.3% were abnormal. The addition of NAA or an increase in its concentration led to a higher occurrence of abnormal plants. After the regenerated shoots were transferred to 1/2 MS medium, roots were observed within 10-15 days. By day 30, they had developed into complete plants. The results obtained from the regenera- tion experiments with the perilla variety Dayu can valuably inform molecular breeding reliant on transformation tech- niques such as genome-editing and genetic modification technology.
Abstract
This study was conducted to establish an efficient plant regeneration system in ‘Dayu’, a Korean variety of Perilla frutescens developed for seed oil production, in conjunction with the previously studied variety ‘Namcheon’. The healthiest callus was formed on the hypocotyl explants cultured on a medium containing 0.1 mg/L NAA and 0.5 mg/L BA, outperforming the leaf and cotyledon samples. In both dark and long-day conditions, Dayu consistently exhibited significantly higher shoot regeneration rates compared with Namcheon. The highest shoot regeneration rates in Dayu were observed from the hypocotyl explants cultured on 0.1 mg/L NAA and 0.5 mg/L BA media, with shoot regeneration rates of 84.4% and 86.7% under dark and long-day conditions, respectively. Various combinations of plant growth regulators were tested to establish the optimal shoot regeneration conditions for Dayu hypocotyl explants. The results demonstrated that the highest shoot regeneration rate (90%) was achieved when 0.5 mg/L of BA was added to the medium without NAA. Among the regenerated shoots, 70.5% were normal plants, while 19.3% were abnormal. The addition of NAA or an increase in its concentration led to a higher occurrence of abnormal plants. After the regenerated shoots were transferred to 1/2 MS medium, roots were observed within 10-15 days. By day 30, they had developed into complete plants. The results obtained from the regenera- tion experiments with the perilla variety Dayu can valuably inform molecular breeding reliant on transformation tech- niques such as genome-editing and genetic modification technology.
참고문헌 (31)
- Ahmed HM, "Ethnomedicinal, phytochemical and pharmacological investigations of Perilla frutescens (L.) Britt", Molecules, 24, pp.102~ (2018)
- Asif M, "Phytochemical study of polyphenols in Perilla Frutescens as an antioxidant", Avicenna J Phytomed, 2, pp.169~178 (2012)
- Bhatia S, "modern applications of plant biotechnology in pharmaceutical sciences", pp.209~230 (2015), Academic Press
- Ha TJ, "Isolation and identification of phenolic compounds from the seeds of Perilla frutescens (L.) and their inhibitory activities against α-glucosidase and aldose reductase", Food Chem, 135, pp.1397~1403 (2012)
- 한호석, "들깨잎의 품종에 따른 성분분석 및 생리활성 물질 탐색", 한국식생활문화학회지, 19(1), pp.94~105 (2004), 한국식생활문화학회
- Heci Y, "Valuable ingredients from herb perilla : A mini review", Innov Food Technol, 29, pp.32~33 (2001)
- Hong YP, "Postharvest changes in quality and biochemical components of perilla leaves", Korean J Food Sci Technol, 18, pp.255~258 (1986)
- Hou SW, "In vitro regeneration of Perilla frutescens from hypocotyl and cotyledon explants", Biol Plant, 49, pp.129~132 (2005)
- Huang S, "The role and mechanism of Perilla frutescens in cancer treatment", Molecules, 28, pp.5883~ (2023)
- Jung CS, "A New vegetable perilla variety"Namcheondlkkae"characterized by short stem and middle-large leaf with high antioxidizing activity", Kor J Breed Sci, 30, pp.403~403 (1998)
- Jung SH, "Changes of RNA and protein during callus induction and plant regeneration from Pefilla fruescens", J Life Sci, 9, pp.29~34 (1999)
- Kaur N, "Essential fatty acids as functional components of foods-a review", J Food Sci Tech, 51, pp.2289~2303 (2014)
- Kim HU, "Fatty acid composition and oil content of seeds from perilla (Perilla frutescens (L.) var. frutescens) germplasm of Republic of Korea", Genet Resources Crop Evol, 66, pp.1615~1624 (2019)
- Kim JA, "Callus induction and plant regeneration from leaf segments and cotyledonary explants of Perilla frutescens", J Plant Biotechnol, 20, pp.47~50 (1993)
- Kim KH, "Agrobacterium-mediated genetic transformation of Perilla frutescens", Plant Cell Reports, 23, pp.386~390 (2004)
- 김경민, "들깨의 자엽절편배양을 통한 고효율 식물체 재분화", Journal of Plant Biotechnology, 34(1), pp.69~73 (2007), 한국식물생명공학회
- Kim MY, "Changes in antioxidant compound and activities of perilla seed, flower and leaf(Perilla frutescens)according to extraction method and solvent", Kor Soc Food Sci Nutr Proceedings, 547 (2022)
- Lee HS, "Effect of low temperature and plant growth regulators on callus induction and plant regeneration in anther culture of perilla", Kor J Breed Sci, 26, pp.345~352 (1994)
- Byoung-Kyu Lee, "Efficient in vitro Shoot Regeneration of Perilla (Perilla frutescens) from Cotyledon, Hypocotyl and Leaf Explants", 한국육종학회지, 35(4), pp.237~240 (2003), 한국육종학회
- 이명희, "기름함량이 높은 종실용 들깨 신품종 ‘다유’", 한국육종학회지, 43(6), pp.616~619 (2011), 한국육종학회
- Long Y, "New insights into tissue culture plant-regeneration mechanisms", Front Plant Sci, 13, pp.926752~ (2022)
- Moon JG, "Effect of growth regulators on plant regeneration from the cotyledon explant in Oriental Melon (Cucumis melo L.)", Kor J Plant Tiss Cult, 27, pp.1~6 (2002)
- Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol Plant, 15, pp.473~497 (1962)
- Pressi G, "A novel Perilla frutescens (L.) Britton cell-derived phytocomplex regulates keratinocytes inflammatory cascade and barrier function and preserves vaginal mucosal integrity in vivo", Pharmaceutics, 15, pp.240~ (2023)
- Siddique AB, "Effect of light and dark on callus induction and regeneration in tobacco (Nicotiana tabacum L.)", Bangladesh J Bot, 44, pp.643~ (2018)
- Skoog F, "Molecular and Cellular Aspects of Development", pp.481~494 (1965), Harper & Row
- H. M. M. Tariq Hossain, "The apical bud as a novel explant for high-frequency in vitro plantlet regeneration of Perilla frutescens L. Britton", Plant Biotechnology Reports, 4(3), pp.229~235 (2010), 한국식물생명공학회
- Yu Y, "Red light promotes cotton embryogenic callus formation by influencing endogenous hormones, polyamines and antioxidative enzyme activities", Plant Growth Regul, 87, pp.187~199 (2019)
- Zenser N, "Auxin modulates the degradation rate of aux", Iaa proteins, 2001, pp.98, 11795~11800
- Zhang T, "In vitro flowering of Perilla frutescens", In Vitro Cell Dev Biol-Plant, 43, pp.91~94 (2007)
- Zhang T, "Plant regeneration in vitro directly from cotyledon and hypocotyl explants of Perilla frutescens and their morphological aspects", Biol Plant, 49, pp.423~426 (2005)