황금배(Pyrus pyrifolia Nakai) 잎 조직으로부터 기내 신초 재분화
In vitro shoot regeneration from leaf tissue of “Whangkeumbae” pear (Pyrus pyrifolia Nakai)
1
조회
0
다운로드
3
인용
0
참고문헌
초록
본 연구는 ‘황금배’(‘Whangkeumbae’)의 엽 절편체로부터형질전환 체계 확립을 위한 효율적인 재분화 체계 확립을 위해 식물 생장조절제와 주요 탄소원인 sucrose,sorbitol이 식물체 재분화에 미치는 영향을 조사하기 위해수행되었다. 잎 절편체를 MS 배지에 TDZ 농도를 각각0.1, 0.25, 0.5, 1, 2.5, 5 mg/L으로 하여 IBA 0.3 mg/L을 혼용처리한 결과 TDZ 0.25 mg/L 농도에서 61.1%의 가장 양호한 재분화율을 보였으며 TDZ 2.5 mg/L 이상의 농도에서는 신초의 재분화율이 급격히 감소하였다. IBA와 IAA 농도에 의한 신초의 재분화율은 TDZ 0.5 mg/L와 IAA 혼용 처리한 구에서 전체적으로 높은 신초의 재분화율을보였으며 특히 TDZ 0.5 mg/L + IAA 0.3 mg/L 처리구에서76.7%의 가장 높은 재분화율을 보였다. 서로 다른 탄소원으로써 sucrose와 sorbitol의 영향에 대하여 15 g/L, 30 g/L 농도로 처리한 결과 sorbitol 30 mg/L에서 가장 높은 재분화율을 보였고 또한 절편체 당 신초의 수도 3.5개로 가장양호하였다. 따라서 sorbitol이 sucrose보다 `황금배`의 신초의 재분화에 있어서 효과적이었다.
Abstract
In order to establish an efficient adventitious shoot regeneration conditions from leaf explants for Asian pear ‘Whangkeumbae’, the effect of concentration and kinds of plant growth regulator and carbon source was investigated. Leaf explants of cultures grown on Murashige and Skoog (MS) medium containing 8 g/L plant agar were used. When the medium contained 0.25 mg/L thidiazuron (TDZ) and 0.3 mg/L indolebutyric acid (IBA), the adventitious shoot regeneration rate (ASRR) was greater as 61.1% than others treated and higher TDZ concentrations (2.5 and 5 mg/L) treatment significantly reduced the ASRR. As the effect of IBA and indoleacetic acid (IAA)concentration on the ASRR, 0.5 mg/L TDZ plus different concentration of IAA exhibited relatively high ASRR and 0.5 mg/L TDZ plus 0.3 mg/L IAA showed the highest ASRR of 76.7%. Also the effect of sucrose and sorbitol as carbon source on regeneration was examined. The highest ASRR and the most shoots per explants averaged 94.4% and 3.49 by treatment of 30 mg/L sorbitol, respectably. Sorbitol is considered better carbon source than sucrose for shoot regeneration of ‘Whangkeumbae’ pear.
참고문헌 (33)
- Ahmad T, "Comparison of sucrose and sorbitol as main carbon energy sources in micropropagation of peach rootstock GF-677", Pak J Bot, 39, pp.1269~1275 (2007)
- Bell RL, "Interaction of genotype and auxin affecting regeneration of pear", Hortscience, 38, pp.750~ (2003)
- Bell RL, "Adventitious shoot regeneration of pear (Pyrus spp.) genotypes", Plant Cell Tissue Organ Cult, 108, pp.229~236 (2012)
- Brown DCW, "Osmotic requirement for shoot formation in tabacco callus", Physiol Plant, 46, pp.36~41 (1979)
- Caboni E, "In vitro shoot regeneration from leaves of wild pear", Plant Cell Tissue Organ Cult, 59, pp.1~7 (1999)
- Caboni E., "In vitro plant regeneration from callus of shoot apices in apple shoot culture", Plant Cell Rep, 19, pp.755~760 (2000)
- Chevreau E, "The effect of mother plant pretreatment and explants choice on regeneration from in vitro pear leaves", Acta Hort, 336, pp.263~268 (1993)
- Chevreau E, "Effect of gelling agents and antibiotics on adventitious bud regeneration from in vitro leaves of pear", In Vitro Cell Dev Biol Plant, 33, pp.173~179 (1997)
- Chevreau E, "Adventitious shoot regeneration from leaf tissue ofthree pear (Pyrus sp.)", Plant Cell Rep, 7, pp.688~691 (1989)
- Feng X, "Effects of sorbitol on expression of genes involved in regeneration of upland rice (Oryza sativa L.)", Plant Cell Tissue Organ Cult, 106, pp.455~463 (2011)
- Garcia R, "Influence of type of explant, plant growth regulators, salt composition of basal medium, and light on callogenesis and regeneration in Passiflora suberosa L. (Passifloraceae) Renata", Plant Cell Tissue Organ Cult, 106, pp.47~54 (2011)
- Geng P, "Effect of sorbitol concentration on regeneration of embryogenic calli in upland rice varieties (Oryza sativa L.)", Plant Cell Tissue Organ Cult, 92, pp.303~313 (2008)
- Hassan MU, "Effect of sorbitol in callus induction and plant regeneration in wheat", Afr J Biotechnol, 8, pp.6529~6535 (2009)
- Hennayake CK, "An Efficient and reproducible in vitro plant regeneration from leaf discs in pear cultivars (Pyrus spp)", Plant Biotechnol, 20, pp.283~289 (2003)
- Huetteman CA, "Thidiazuron: a potent cytokine for woody plant tissue culture", Plant Cell Tissue Organ Cult, 33, pp.105~119 (1993)
- Kadota M, "Double-phase in vitro culture using sorbitol increases shoot proliferation and reduces hyperhydricity in Japanese pear", Sci Hort, 89, pp.207~215 (2001)
- Kadota M, "Effect of cytokinin types and their concentrations on shoot proliferation and hyperhydricity in in vitro pear cultivar shoots", Plant Cell Tissue Organ Cult, 72, pp.261~265 (2003)
- Keulemans J, "Plant regeneration from cotyledons and embryonic axes in apple: Sites of reaction and effect of pre-culture in the light", Euphytica, 77, pp.135~139 (1994)
- 김정희, "과수 형질전환 국내 연구 동향 및 전망", Journal of Plant Biotechnology, 37(4), pp.408~413 (2010), 한국식물생명공학회
- Lane WD, "Regeneration of pear plants from shoot meristem- TIP", Plnat Sci Lett, 16, pp.337~342 (1979)
- Lane WD, "Shoot regeneration from cultured leaves of Japanese pear (Pyrus pyrifolia)", Plant Cell Tissue Organ Cult, 54, pp.9~14 (1998)
- Leblay C, "Adventitious shoot regeneration from in vitro leaves of several pear cultivars (pyrus communis L.)", Plant Cell Tissue Organ Cult, 25, pp.99~105 (1991)
- Chang-Hoo Lee, "배(Pyrus pyrifolia) '신고' 잎으로부터 효과적인 재분화 시스템 개발", Horticulture, Environment, and Biotechnology, 43(3), pp.271~274 (2002), 한국원예학회
- Lemos EEP, "Shoot regeneration in response to carbon source on intermodal explants of Annona muricata L", Plant Growth Regul, 25, pp.105~112 (1998)
- Marino G, "Comparative effects of sorbitol and sucrose as main carbon energy sources in micropropagation of apricot", Plant Cell Tissue Organ Cult, 34, pp.235~244 (1993)
- Mukherjee SK, "Low sugar and osmotic requirements for shoot regeneration from leaf piece of Solanum melongena L", Plant Cell Tissue Organ Cult, 25, pp.13~16 (1991)
- Murashige T, "A revised medium for rapid growth and bioassay with tobacco tissue cultures", Physiol Plant, 15, pp.473~497 (1962)
- Ochatt SJ, "Plant regeneration from mesophyll protoplasts of Williams Bon chretien (syn. Bartlett) pear(Pyrus communis L.)", Plant Cell Rep, 7, pp.587~589 (1988)
- Predieri S, "High-frequency shoot regeneration from leaves of the apple rootstock M26 (Malus pumila Mill)", Plant Cell Tissue Organ Cult, 17, pp.133~142 (1989)
- Saito A, "Plant regeneration from meristem-derived callus protoplasts of apple (Malus x domestica cv. ‘Fuji’)", Plant Cell Rep, 18, pp.549~553 (1999)
- Sotiropoulos TE, "Sucrose and sorbitol effects on shoot growth and proliferation in vitro, nutritional status and peroxidase and catalase isoenzymes of M 9 and MM 106 Apple (Malus domestica Borkh.) rootstocks", Europ J Hort Sci, 71, pp.114~119 (2006)
- Swedlund B, "Sorbitol as the primary carbon source for the growth of embryogenic callus of Maize", Plant Physiol, 103, pp.1339~1346 (1993)
- Zhang P, "In vitro explants regeneration of the grape ‘Wink’ (Vitis vinifera L.)", J Plant Breed Crop Sci, 3, pp.276~282 (2011)