Evaluating phylogenetic relationships in the Lilium family using the ITS marker
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Lilium is a perennial bulbous plant belonging to the liriotypes genus. Our aim was to study the phylogenetic relationships of the Lilium family. Two varieties of Lilium ledebourii, 44 varieties of the gene bank, and one variety from the Tulipa family served as the out group. In order to study the diversity between lilium masses, ITS regions were used to design the marker. The results showed that the guanine base is the most abundant nucleotide. Relatively high conservation was observed in the ITS regions of the populations (0.653). Phylogenetic analysis showed that sargentiae and hybrid varieties are older than other varieties of the Lilium family. Also, the location of L. ledebourii varieties (Damash and Namin) was identified in a phylogenetic tree by using the ITS marker. Overall, our research showed that ITS molecular markers are very suitable for phylogenetic studies in the Lilium family.
Abstract
Lilium is a perennial bulbous plant belonging to the liriotypes genus. Our aim was to study the phylogenetic relationships of the Lilium family. Two varieties of Lilium ledebourii, 44 varieties of the gene bank, and one variety from the Tulipa family served as the out group. In order to study the diversity between lilium masses, ITS regions were used to design the marker. The results showed that the guanine base is the most abundant nucleotide. Relatively high conservation was observed in the ITS regions of the populations (0.653). Phylogenetic analysis showed that sargentiae and hybrid varieties are older than other varieties of the Lilium family. Also, the location of L. ledebourii varieties (Damash and Namin) was identified in a phylogenetic tree by using the ITS marker. Overall, our research showed that ITS molecular markers are very suitable for phylogenetic studies in the Lilium family.
참고문헌 (28)
- Adams BJ, "phylogenetic analysis of Heterorhabditis (Nemata: Rhabditidae) based on internal transcribed spacer 1 DNA sequence data", Nematol, 30, pp.22~29 (1998)
- Adlard RD, "Comparison ofthe second internal transcribed spacer (ribosomal DNA) from populations and species of Fasciolidae (Digenea)", International J for Parasitol, 23, pp.423~425 (1993)[DOI]
- Ainouche AK, "Phylogenetic relationships in Lupinus (Fabacea: Papilionoideae) Based on internal transcribed spacer (ITS) of nuclear ribosomal DNA", American J Botany, 86(4), pp.590~607 (1999)[DOI]
- Arzanlou M., "Phylogeny, detection and mating behavior of Mycosphaerella spp occurring on banana", pp.2008
- Baranova MV., "A synopsis of the system of the genus Lilium (Liliaceae)", Bot Zurn, 73, pp.1319~1329 (1988)
- Brasileiro B, "Genetic variability within Fusariumsolani species as revealed by PCR-Fingerprint based on PCR-markers", Brazilian J Microbial, 35, pp.205~210 (2004)[DOI]
- Comber HF., "A new classifi cation of the genus Lilium", Lily Year-Book, 13, pp.86~105 (1949)
- Crous PW, "Fungal biodiversity. CBS Laboratory Manual Series 1", pp.269~ (2004), Centraalbureau voor Schimmelcultures
- Cunnington HJ, "Unexpected ribosomal DNA internal transcribed spacer sequence variation within Erysipheaquilegiaesensulato", Fungal Diversity, 16, pp.1~10 (2004)
- Davari M, "Rapid identification of Fusarium graminearum species complex using Rolling Circle Amplification (RCA)", J Microbiol Methods, 89, pp.63~70 (2012)[DOI]
- Doyle JJ, "A rapid DNA isolation procedure for small quantitiesof fresh leaf tissue", Phytochem Bull, 19, pp.11~15 (1987)
- Dubouzet JG, "Phylogenetic analysis of the Internal Transcribed Spacer region of Japanese Lilium species", Theor Appl Genet, 98, pp.954~960 (1999)[DOI]
- Feng T, "Molecular authentication of the traditional Chinese medicinal plant Angelica sinensis based on internal transcribed spacer of nrDNA", Electronic J Biotech, 13, pp.1~13 (2009)
- Frezal L, "Four years of DNA barcoding: Current advances and prospects", Infect Genet Evol, 8, pp.727~736 (2008)[DOI]
- Gultepe M, "Internal transcribed spacer (ITS) polymorphism in the wildPrimula(Primulaceae) taxa of Turkey", Turkish J Botany, 34, pp.147~157 (2010)
- Hebert PDN, "Biological identifications through DNA barcodes", Proc R Soc Lond B, 270, pp.313~321 (2003)[DOI]
- Ikinci N, "Molecular phylogeny and divergence times estimates of Lilium section Liriotypus (Liliaceae) based on plastid and nuclear ribosomal ITS DNA sequence data", Turk J Bot, 35, pp.319~330 (2011)
- Ikinci N, "Genetic relationships among NE Turkish Lilium L. (Liliaceae) species based on a Random Amplifi ed Polymorphic DNA (RAPD) analysis", Pl Syst Evol, 284, pp.41~48 (2010)[DOI]
- Joseph N, "Ribosomal internal transcribed spacer 2 (ITS2) exhibits a common core of secondary structure in vertebrates and yeast", Nucleic Acids Research, 27, pp.4533~4540 (1999)[DOI]
- Luikart G, "The power and promise of population genomics: from genotyping to genome typing. Nature reviews", Genetics, 4(12), pp.981~994 (2003)
- Ma X, "Authentic identification of stigma Croci (stigma of Crocus sativus L.) from its adulterants by molecular genetic analysis", Planta Med, 2, pp.183~186 (2006)
- McRae EA, "Lilies: A Guide for Growers and Collectors", pp.392~ (1998), Timber Press
- Nishikawa T, "A molecular phylogeny of Lilium in the Internal Transcribed Spacer region of nuclear ribosomal DNA", J Mol Evol, 49, pp.238~249 (1999)[DOI]
- Picoult-Newberg L, "Mining SNPs from EST databases", Genome Research, 9(2), pp.167~174 (1999)
- Resetnik I, "Molecular phylogeny and systematics of the Lilium carniolicum group (Liliaceae) based on nuclear ITS sequences", Pl Syst Evol, 265, pp.45~58 (2007)[DOI]
- Ronsted N, "Molecular phylogenetic evidence for the monophyly of Fritillaria and Lilium (Liliaceae; Liliales) and the infrageneric classification of Fritillaria", Mol Phyl Evol, 35, pp.509~527 (2005)[DOI]
- Taylor JW, "Phylogenetic species recognition and species concepts in fungi", Fungal Genet Biol, 31, pp.21~32 (2000)[DOI]
- Termorshuizen AJ, "On the nomenclature of the European species of the Armillaria mellea group", Mycotaxon, 30, pp.101~106 (1997)