A Phylogenetic Study of Scirpus planiculmis F. Schm. (Cyperaceae) Based on ITS1 Sequences of Nuclear Ribosomal DNA
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참고문헌
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초록
This work was performed to confirm the molecular discrimination through the nrITS1 sequences among 3 taxa of Scirpus L. sensu lato (s.l.) species. S. planiculmis represented only 2 base sequence variations with S. maritimus in spite that they showed different morphological features. The nucleotide sequences of the ITS1 region from S. planiculmis were shown to have 99.1% homology with S. maritimus and 60.4% homology with S. triqueter. Although the morphology of S. planiculmis is similar with S. triqueter, molecular basis of the size and sequences on ITS1 region were shown to have distinctive differences. For divergency investigation on same sites and metapopulation, sequencing was conducted on ITS1 region with partial 5.8S and 18S regions. All plants of each species collected at the same site had identical band size pattern and sequences. Intraspecific molecular divergency was not identified in spite that these species live in different wetland sites. The ITS1 sequences described here provided a powerful genetic tool for phylogenetic studies which was difficult by morphological identification as high rate of morphological plasticity
Abstract
This work was performed to confirm the molecular discrimination through the nrITS1 sequences among 3 taxa of Scirpus L. sensu lato (s.l.) species. S. planiculmis represented only 2 base sequence variations with S. maritimus in spite that they showed different morphological features. The nucleotide sequences of the ITS1 region from S. planiculmis were shown to have 99.1% homology with S. maritimus and 60.4% homology with S. triqueter. Although the morphology of S. planiculmis is similar with S. triqueter, molecular basis of the size and sequences on ITS1 region were shown to have distinctive differences. For divergency investigation on same sites and metapopulation, sequencing was conducted on ITS1 region with partial 5.8S and 18S regions. All plants of each species collected at the same site had identical band size pattern and sequences. Intraspecific molecular divergency was not identified in spite that these species live in different wetland sites. The ITS1 sequences described here provided a powerful genetic tool for phylogenetic studies which was difficult by morphological identification as high rate of morphological plasticity
참고문헌 (19)
- Amat, J. A., "Effects of wintering graylag geese Anser anser on their Scirpus food plants", Ecography, 18, pp.155~163 (1995)
- Gibson, T., "Clustal X program" (1994), EMBL Hidelberg Germany
- Groenendijck, A. A, "Evaluation of one year research on the foodplant biomass for waterfowl in the Nakdong estuary, republic of Korea. In: Nakdong estuary barrage and reclamation project", pp.56~ (1985), Delta Institute NEDECO
- Hipp, A. L., "Phylogeny and patterns of convergence in Carex sect. Ovales (Cyperaceae): evidence from ITS and 5.8S sequences, In Sedges: uses, diversity, and systematics of the Cyperaceae", pp.197~214 (2008), Missouri Botanical Garden
- Hirahara, T., "Suprageneric phylogeny of Japanese Cyperaceae based on DNA sequences from chloroplast ndhF and 5.8S nuclear ribosomal DNA", Acta Phytotaxon Geobot, 58, pp.57~68 (2007)
- Jongduk Jung, "Systematic Rearrangement of Korean Scirpus L. s.l. (Cyperaceae) as Inferred from Nuclear ITS and Chloroplast rbcL Sequences", Journal of Plant Biology(한국식물학회지), 53(3), pp.222~232 (2010), 한국식물학회
- Kim, C. K., "Cloning and structural analyses of partial nuclear rDNA from Bulpleurum euphorbioides (Apiaceae)", J. Plant Biol, 42, pp.133~138 (1999)
- Koichiro, T., "MEGA 4: Molecular evolution genetics analysis (MEGA) software version 4.0", Biol. Evol, 24, pp.1596~1599 (2007)
- Muasya, A. M., "An assessment of suprageneric phylogeny in Cyperaceae using rbcL DNA sequences", Plant Syst Evol, 211, pp.257~271 (1998)
- Muasya, A. M., "Phylogeny of Cyperaceae based on DNA sequence data: current progress and future prospects", Bot Rev (Lancaster), 75, pp.2~21 (2009)
- Roalson, E. H., "Phylogenetic relationships in Cariceae (Cyperaceae) based on ITS (nrDNA) and trnTL-F (cp DNA) region sequences : assessment of subgeneric and sectional relationships in Carex with emphasis on section Acrocystis", Syst Bot, 26, pp.318~341 (2001)
- Simpson, D. A., "Frosted curls to tiger nuts: ethnobotany of Cyperaceae, In Sedges: uses, diversity, and systematics of the cyperaceae", pp.1~14 (2008), Missouri Botanical Garden
- Simpson, D. A., "Phylogeny of Cyperaceae based on DNA sequence data-a new rbcL analysis", Aliso, 23, pp.72~83 (2007)
- Strong, M. T., "New combination in Schoenoplectus (Cyperaceae)", Novon, 3, pp.202~203 (1993)
- Suh, Y., "DNA and reconstruction of plant phylogeny", Kor. J. Plant Tax, 22, pp.121~140 (1992)
- Suh, Y., "Comparative analysis of ITS sequence from Acer species (Aceraceae) in Korea", J. Plant Biol, 39, pp.1~8 (1996)
- Suh Y, "A phylogenetic study of Polygonum sect. Tovara (Polygonaceae) based on ITS sequences of nuclear ribosomal DNA", J. Plant Biol, 40, pp.47~53 (1997)
- Yano, O., "Molecular phylogeny and chromosomal evolution of Japanese Schoenoplectus (Cyperaceae), based on ITS and ETS 1f sequences", Acta Phytotaxon Geobot, 56, pp.183~195 (2005)
- Yoon, H. S., "Estimation of foodplant biomass and their use by waterfowl in the Nakdong estuary", 박사, pp.1987