철포백합의 기내 체세포 염색체 배수화를 위한 화학처리의 효과
In Vitro Mitotic Chromosome Doubling by Chemical Treatments in Lilium Iongiflorum
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초록
본 연구에서는 백합의 배수성 육종 체계를 확립하기 위한 기초자료를 얻기 위하여 인위적 체세포 염색체 배수화 방법 에 대한 실험을 수행하였다. 체세포 배수화를 위한 in vitro 처 리의 경우 4배체 뿐 아니라 혼수체의 비율이 처리에 따라 높 게 나타나는 경우도 있으며, 무엇보다 4배체 획득율을 기준으 로 그 효율성 조사해 본 결과 colchicine의 경우 1,000 ppm 농 도에서 3시간 처리 (효휼성＝11.2%), oryzalin의 경우 30 ppm 에서 3시간 처리 (효율성＝10.0%), caffeine의 경우 9,000 ppm 에서 9시간 처리 (효율성＝11.7%)가 가장 양호하였다. 처리에 따른 4배체 획득 요율성은 다소 차이가 있을 수 있으나 인체 에 대한 유해성, 혼수체 빈도 및 식물체에 대한 toxicity 등을 고려 할 때 oryzalin 또는 caffeine 사용이 바람직할 것으로 판 단된다.
Abstract
This study was carried out to develop an in vitro chromosome doubling systems for the breeding oftetraploid lily cultivar. Different concentration (ppm) and treatment time (hour) of colchicine, oryzalin and caffeinewere compared for the efficiency of bulb regeneration and tetraploidization. The occurrence of tetraploid plants bycolchicine was the highest in the concentration of 1,000 ppm for three hours. In oryzalin treatment, the bestcombination was at 30 ppm for three hours. However, the effects of oryzalin treatments were similar between 10ppm and 30 ppm concentrations. The survival rate was dramatically reduced in a high concentration of caffeine,although some treatments had a higher tetraploid induction. As a consequence, reliable results for the tetraploidproduction were obtained in the treatments of 1,000 ppm colchicine, 3,000 ppm oryzalin both for three hours, and9,000 ppm caffeine for nine hours.
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