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체세포 염색체가 배가된 4배체 Oriental-Asiatic(OA) hybrid 유래 종간잡종 백합의 후대검정

Progeny Analysis of Hybrid Lilies Crossed Between Several Genotypes and Tetraploid Oriental-Asiatic Hybrids

정재동|임기병|정미영|Jaap M. Van Tuyl
한국육종학회 (The Korean Society of Breeding Science)|2010.03|한국육종학회지 (KOREAN JOURNAL OF BREEDING SCIENCE)|제42권 제1호|pp. 100-108

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키워드

이입recombination아시아틱여교잡GISH조환Asiaticback crossintrogression
주제분류농학

초록

본 연구는 체세포 배수화를 통해 생산한 4배체 OA hybrid 와 다양한 교배조합간 후대를 대상으로 염색체 구성 및 조환여부를 알아보기 위하여 수행되었다. 4배체 OA hybrid의 여교잡 후대를 분석한 결과, 2배체 아시아틱 품종을 부 또는 모본으로 정역교배한 조합에서는 3배체가, 4배체 아시아틱과 4배체 OA 품종을 부 또는 모본으로 한 교배조합에서는 4배체가 생산되었다. 그러나, 2배체 Oriental 품종을 모본으로 교배한 경우, 3배체 또는 2배체를 얻을 수 있었다. OA hybrid를대상으로 GISH방법으로 이종염색체의 조환여부를 확인한 결과, OA hybrid의 염색체수는 24개, 그 중 12개는 모본인Oriental hybrid, 12개는 부본인 Asiatic hybrid에서 유래한것을 알 수 있었다. 체세포 염색체간 배수화된 OA hybrid의여교잡 후대의 경우, A × OA, OA × A 교배에서는 Oriental hybrid 염색체가 12개, Asiatic hybrid 24개, OA × OA간 교배에서는 Oriental hybrid 염색체 24개와 Asiatic hybrid 24 개로 진정한 4배체 잡종임을 확인 할 수 있었으며, 이들 후대는 어떠한 조합에서도 부분적인 이종염색체간 조환이 일어나지 않았다. 한편 O × OA 교배의 후대식물의 염색체 구성은Oriental hybrid 유래 염색체만 2x=24개인 경우와 3배체(Oriental = 24, Asiatic = 12)로 판명되었다. 4배체 아시아틱계 품종을 모본으로 한 AA × OA 교배조합의 경우, Asiatic hybrid염색체만 48개로 나타났다.

Abstract

This study was carried out to confirm the chromosome constitution and homoeologous recombination of progenies derived from various cross combination using tetraploid OA interspecific hybrid originated from mitotic chromosome doubling. Based on the chromosome analysis of progenies crossed reciprocally, there were only triploid progenies when crossed with diploid Asiatics as male or female parent. While only tetraploid progenies were produced when crossed tetraploid Asiatics or tetraploid OA hybrid with tetraploid OA hybrid, respectively. However, two types of progenies, that is, diploid and triploid plants,were produced from cross combinations between diploid Oriental hybrid and tetraploid OA hybrid. From the GISH analysis of OA hybrid, it was confirmed that diploid F1 OA hybrid was consisted of 24 chromosomes (12 Oriental and 12 Asiatics) showing authentic OA hybrid. On the other hand, it was notified that triploid plants (3x=36) were consisted of 24 Asiatics lily chromosomes and 12 Oriental lily chromosomes by analysis of backcross progenies derived from either A×OA or OA×A crosses. In cross between tetraploid OA and OA, all the progenies were tetraploid with equal number of chromosomes without any homoeologous recombination, i.e. each 24 chromosomes of Oriental and Asiatics. In 2x-4x (O×OA) cross combination, some progenies had 2x=24 chromosomes originated from only Oriental hybrid, and other progenies had 3x=36 chromosomes derived from 24 chromosomes of Oriental hybrid and 12 chromosomes of Asiatic hybrid. Only tetraploid Asiatics chromosomes without any Oriental one were produced in all the progenies from 4x-4x (AA×OA) cross combination.

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체세포 염색체 배가법을 이용한 백합 OA 종간 잡종의 불임극복과 후대획득임기병, 정미영, 정재동, 지선옥, J.M. Van Tuyl한국식물생명공학회 · Journal of Plant Biotechnology2004 · 제31권 제2호자발적 생식세포 염색체 배수화 기작에 의한 Oriental-Asiatic hybrid(OA) F1 종간잡종의 임성회복과 후대검정임기병, 정미영, 정재동, Jaap M. Van Tuyl한국육종학회 · 한국육종학회지2009 · 제41권 제3호철포백합의 기내 체세포 염색체 배수화를 위한 화학처리의 효과임기병, 정미영, 정재동, J.M. Van Tuyl한국식물생명공학회 · Journal of Plant Biotechnology2004 · 제31권 제1호아시아틱 나리 신품종 육성을 위한 다양한 품종간 교잡김홍열, 손병구, 임기병, 조영관, Fahad Ramzan한국육종학회 · 한국육종학회지2018 · 제50권 제1호GISH와 FISH를 이용한 양파와 파간 종간교잡계통의 염색체 분석구달회, 김철우, 김화영, 방재욱, 방진기, 이을태, 최인후한국육종학회 · 한국육종학회지2009 · 제41권 제4호소핵을 가진 노란 화색의 이수성 호접란의 꽃가루 생식력 분석라이사, 사만다, 안혜련, 황윤정한국육종학회 · 한국육종학회지2023 · 제55권 제4호

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