체세포 염색체 배가법을 이용한 백합 OA 종간 잡종의 불임극복과 후대획득
Overcoming F₁Sterility of Intersectional OA Lily Hybrids by Somatic Chromosome Doubling and Production of Subsequent Progeny
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본 연구는 나리의 종간잡종 육성체계를 확립하기 위한 기초자료를 얻고자 Oriental과 Asiatic 잡종 (OA-hybrid) 의 후대 획득에 필요한 실험을 수행하여 얻은 결과이다. 체세포 배수화를 통한 이질 4배체 OA-hybrid 3계통으로 화분발아 실험을 수행한 결과, 0～80% 범위로 계통간 차이가 현저하였다. 이질 4배체 OA-hybrid를 자방친 또는 화분친으로 사용하고 Asiatic 또는 Oriental hybrid와 교배시 후대 획득수에 차이가 있었는데 4배체 OA-hybrid를 화분친으로 이용하였을 때, 그리고 상대친을 Asiatic hybrid로 교배하였을 때 후대획득이 현저히 용이하였다. 그러나, 이질 4배체 F1 OA-hybrid를 자방친으로 사용하거나 Oriental hybrid와 교배하면 후대 획득률이 현저히 저하 되거나 또는 전혀 후대생산이 이루어지지 않았다. 이질 4배체 F1 OA-hybrid와 교배시 상대친의 ploidy level은 4배체 보다는 2배체가 유리하였다.
Abstract
This study was carried out to develop the efficient methods for the subsequent progeny of intersectional hybrid between Oriental and Asiatic lily hybrids. The pollen fertility and germination ability of 3 different allotetraploids (OAOA) after somatic chromosome doubling was ranged from 0 to 80 percent on artificial pollen germination medium. The number of BC1 progeny using allotetraploid of F1 OA-hybrid as male and female parent was different. The efficiency of BC1 progeny production was increased when F1 OA-hybrids was used as male rather than as female parent. And in back crosses of F1 OA-hybrids with the Asiatic and Oriental hybrids, Asiatic hybrids showed higher efficiency on BC1 progeny production. The ploidy level between 2x or 4x Asiatic hybrid and allotetraploid F1 OA-hybrid was determined and showed higher progeny production in 2x-4x crosses rather than 4x-4x.
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