세 가지 나리 종간교잡의 자방배양에서 사이토키닌농도별 신초형성 효과
Shoot Development thorough Cytokinin Treatments in Ovary Culture of Three Interspecific Hybrid Lilies
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초록
본 연구는 나리 주요 품종의 종간교잡에서 생기는 삭과의 배유 퇴화방지와 미숙배 형성 시 발생하는 이상증상을 경감하기위해 나리 종간교잡계통의 자방배양 했을 때 cytokinin 첨가가신초형성에 미치는 영향을 구명하고자 하였다. FO계통은 BA 2.0 ㎎/L에서 신초 발생율이 18%로 가장 효과적이었고, BA 3.0 ㎎/L와 5.0 ㎎/L에서는 12%로 다소 저조한 결과였다. TDZ 첨가의 경우는 대조구와 같거나 비슷한 경향이었다. FA계통은 TDZ 첨가 시 대체적으로 신초형성율이 높았고 0.3 ㎎/L 처리가 효과적이었다. 반면 BA를 첨가한 배지에서는 14.3%에서 15.7%로 대조구와 큰 차이가 없었다. FLA계통에서 신초발생율은 생장조절제에 의해 증가되었으나 대조구 20%와 비교했을 때 BA 20~ 24%, TDZ 22~24%로 처리효과는 미미하였다.
Abstract
We performed the following experiment to increase the development of shoots in the in vitro culture of hybrid progeny. MS medium was used as the base medium; cytokinins BA at a concentration of 2.0, 3.0, or 5.0 ㎎/L and TDZ at a concentration of 0.2, 0.3, or 0.5 ㎎/L were added to the in vitro cultures. For the FO progeny, the shoot development rate was highest of 18% with 2.0 ㎎/L BA, at 18%, and 3.0 ㎎/L and 5.0 ㎎/L BA resulted in a lower rate of 12%. TDZ addition tended to identical or similar results to that of the control media. For the FA progeny, addition of TDZ tended to lead to a higher shoot development rate and was superior with 0.3 ㎎/L treatment. However, the addition of BA to the media resulted in a shoot development rate between 14.3% and 15.7%, similar to that of the control.
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