Methyl Jasmonate 및 Salicylic Acid 처리에 의한 더덕(Codonopsis lanceolata) 부정근의 항산화 활성 증가
Increasement of antioxidative activity in Codonopsis lanceolata adventitious root treated by Methyl jasmonate and salicylic acid
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초록
Traditionally, Codonopsis lanceolata root have been used as a source of natural heath food. This study was initiated to investigate the impacts of methyl jasmonate (MeJA) and salicylic acid (SA) on adventitious growth C. lanceolata, the production of secondary metabolites, such as flavonoids, total phenolic compound, antioxidative activity (DPPH). The highest phenolics content was observed in treatment of 20 uM MeJA (74.53 mg/g). The content of total flavonoids followed the similar pattern as that of total phenolics, showing 38.45 mg/g of C. lanceolata treated by 20 uM MeJA. The DPPH scavenging activity was 24.2 (IC50) of C. lanceolata treated by 20 uM MeJA. These results provide useful information for enhancing biological properties of cultural roots of C. lanceolata.
Abstract
Traditionally, Codonopsis lanceolata root have been used as a source of natural heath food. This study was initiated to investigate the impacts of methyl jasmonate (MeJA) and salicylic acid (SA) on adventitious growth C. lanceolata, the production of secondary metabolites, such as flavonoids, total phenolic compound, antioxidative activity (DPPH). The highest phenolics content was observed in treatment of 20 uM MeJA (74.53 mg/g). The content of total flavonoids followed the similar pattern as that of total phenolics, showing 38.45 mg/g of C. lanceolata treated by 20 uM MeJA. The DPPH scavenging activity was 24.2 (IC50) of C. lanceolata treated by 20 uM MeJA. These results provide useful information for enhancing biological properties of cultural roots of C. lanceolata.
참고문헌 (34)
- 안창호, "더덕 (Codonopsis lanceolata)의 부정근 유도 및 생장에 미치는 배양조건과 생물반응기 배양", Journal of Plant Biotechnology, 35(2), pp.155~161 (2008), 한국식물생명공학회
- Ahn CS, "Isolation and culture of protoplast from Platycodon grandiflorum(Jacq) A.D.C. and Codonopsis lanceolata (S. et Z.) Trautv", J Korean Soc Hort Sci, 27, pp.205~212 (1986)
- Branen AL, "Toxicology and biochemistry of butylated hydroxy anisol and butylated hydroxytoluene", J Am Oil Chem Soc, 52, pp.59~63 (1975)
- Se Eun Byeon, "Inhibitory Effect of Saponin Fraction from Codonopsis lanceolata on Immune Cell-Mediated Inflammatory Responses", Archives of Pharmacal Research, 32(6), pp.813~822 (2009), 대한약학회
- Choe SY, "Toxicological studies of antioxidants butylated hydroxytoluene (BHT) and butylated hydroxy anisol (BHA)", Korean J Food Sci Technol, 14, pp.283~288 (1982)
- Famer EE, "Interplant communication: air-borne methyl jasmonate induces synthesis of proteinase inhibitors in plant leaves", Proc Natl Acad Sci USA, 87, pp.7713~7716 (1990)
- Gutfinger T, "Polyphenols in olive oils", JAOCS, 58, pp.966~967 (1981)
- Jang JG, "Sanyacho Dongeubogam", pp.156~157 (2001), Academy press Seoul
- 정래승, "증숙 및 발효 더덕의 이화학적 특성 및 생리활성 변화", 한국식품과학회지, 44(1), pp.135~139 (2012), 한국식품과학회
- Ketchum REB, "The kinetics of taxoid accumulation in cell suspension cultures of Taxus following elicitation with methyl jasmonate", Biotechnol Bioeng, 62, pp.97~105 (1999)
- 김나영, "더덕껍질의 일반성분 분석과 항산화 활성", 한국식품영양과학회지, 39(11), pp.1627~1633 (2010), 한국식품영양과학회
- 김수현, "더덕(Codonopsis lanceolata) 추출물의 in vitro 및 in vivo 항산화 효과", 한국식품영양과학회지, 39(2), pp.193~202 (2010), 한국식품영양과학회
- Salt-stress tolerances of rice (Oryza sativa L.) and Cucumber (Cucumis sativus L.) affected by nitrogen and salicylic acid, "Kim YH", Graduate of University of seoul, ~ (2010)
- Kim YS, "Methyl jasmonate increase saponin content in bioreactor culture og ginseng (Panax ginseng C.A. Meyer) adventitious roots", Acta Hort, 625, pp.289~292 (2003)
- Kim YS, "Adventitious root growth and ginsenoside accumulation in Panax ginseng cultures as affected by methyl jasmonate", Biotechnol Lett, 26, pp.1619~1622 (2004)
- Kwak SS, "Acidic peroxidases from suspension-cultures of sweet potato", Phytochemi, 39, pp.981~984 (1995)
- 이현화, "흰민들레와 서양민들레 추출물의 세포독성 및 항산화 활성 비교", 한국약용작물학회지, 16(2), pp.79~85 (2008), 한국약용작물학회
- 이종화, "림포사이트 및 클로털 마크로파지계를 모델로 한 더덕열수추출물의 면역증강작용", 한국식품과학회지, 34(4), pp.732~736 (2002), 한국식품과학회
- Lee MS, "Studies on the anther culture of Codonopsis lanceolata", Korean J Plant Tiss Cult, 7, pp.9~12 (1980)
- Li JP, "Triterpenoid saponins and anti-inflammatory activity of Codonopsis lanceolata", Pharmazie, 62, pp.463~466 (2007)
- Maeng YS, "Antioxidant activity of ethanol extract from deodeok (Codonopsis lanceolata)", Kor J Food Sci Technol, 23, pp.311~316 (1991)
- Meyer A, "Occurrence of the plant growth regulator jasmonic acid in plant", J Plant Growth reg, 3, pp.1~8 (1984)
- Min SR, "High frequency somatic embryogenesis and plant regeneration in tissue cultures of Codonopsis lanceolata", Plant Cell Rep, 10, pp.621~623 (1992)
- Moreno MIN, "Comparison of the free radical scavenging activity of propolis from several region of Argentiana", J Enthropharmacol, 71, pp.109~114 (2000)
- Mueller MJ, "Signaling in the elicitation process is mediated through the octadecanoid pathway leading to jasmonic acid", Proc Natl Acad Sci USA, 90, pp.7490~7494 (1993)
- Murashige, T, "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol Plant, 15, pp.473~479 (1962)
- 박성진, "발효더덕 추출물의 생리활성", 한국식품영양과학회지, 38(8), pp.983~988 (2009), 한국식품영양과학회
- Pratt DE, "Phenolic compounds in food and their effects on health (Ⅱ). In Antioxidants and Cancer Prevention", pp.54~71 (1992), American Chemical Society
- Ushijima M, "Triterpene glycosides from roots of Codonopsis lanceolata", Chem Pharm Bull, 56, pp.308~314 (2008)
- Wang W, "Manipulation of ginsenoside heterogeneity in cell cultures of Panax notoginseng by addition of jasmonates", J Biosci Bioeng, 93, pp.48~53 (2002)
- Yongxu S, "Structural characterization of a water-solu-ble polysaccharide from the Roots of Codonopsis pilosula and its immunity activity", Int J Biol Macromol, 43, pp.279~282 (2008)
- Yukimune Y, "Methyl jasmonateinduced overproduction of paclitaxel and ba-ccatin Ⅲ in Taxus cell suspension cultures", Nat Bio-technol, 14, pp.1129~1132 (1996)
- Yu KW, "Jasmonic acid improving ginsenoside accmulation in adventitious root culture of Panax ginseng C.A. Meyer", Biochem Eng J, 11, pp.211~215 (2002)
- Yu KW, "Production of adventitious ginseng roots using bioreactors", Kor J Plant Tiss Cult, 27, pp.309~315 (2000)
