생물반응기내의 공기주입량 및 Sparger 형태가 인삼 (Panax ginseng C.A. Meyer) 부정근의 생장과 Ginsenoside 함량에 미치는 영향
Effects of Aeration Rate and Sparger Type on Growth and Ginsenoside Accumulation in Bioreactor Cultures of Ginseng Adventitious Root (Panax ginseng C.A. Meyer)
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생물반응기 배양을 통하여 인삼 부정근을 대량생산하고자 할 때, 가장 이상적인 공기주입량의 조절은 배양초기부터 말기까지 각각의 농도 (0.05, 0.1, 0.2, 0.3 vvm)를 가지고 동일하게 주입하는 방법보다는 인삼 부정근의 생장이 증가함에 따라 공기주입량을 약 10일 간격을 두고 0.05～0.3 vvm으로 서서히 증가시키는 것이 인삼 부정근의 생장 (175.8 g dry wt)과 총 ginsenoside 함량 (4.3 mg/g dry wt)을 최대로 증가시켰다. 또한 생물반응기내 sparger의 다공 크기를 15, 30, 60 ㎛으로 각각 제작하여 배양하였을 경우, 인삼 부정근의 생장은 15 ㎛ sparger (175.9 g dry wt)에서 양호하였으나 총 ginsenoside 함량은 60 ㎛ sparger (4.9 mg/g dry wt)에서 우수하게 나타났다. 마지막으로 sparger의 직경을 1.5, 3.0, 5.0, 8.0 cm로 각각 제작하여 배양하였을 때 (공기주입량은 0.05～0.3 vvm, sparger의 다공크기는 15 ㎛로 채택), 8.0 cm sparger에서 인삼 부정근의 생장 (191.9 g dry wt)과 총 ginsenoside 함량 (4.9 mg/g dry wt)이 다른 처리구에 비하여 가장 우수하게 나타났다.
Abstract
The two different ways to supply air inside the bioreactor were examined in the adventitious root cultures of Panax ginseng C.A. Meyer. First, the aeration rate varied at 0.05, 0.1, 0.2 and 0.3 vvm, respectively which were supplied during the whole culture period. Second, the amount of air supply was increased from 0.05 to 0.3 vvm at 10-day intervals in proportion to the root growth. Both the root growth and the ginsenoside accumulation were maximized to 175.8 g dry wt. of root growth and 4.3 mg/g dry wt. of ginsenoside accumulation when the aeration rate was increased gradually. The effect of the sparger pore size (15, 30 and 60 m) in the bioreactor was also investigated, which suggested the greatest root growth (175.9 g dry wt.) in the 15 m-sized sparger and the highest ginsenoside content (4.3 mg/g dry wt.) in the 60 m size. Finally, the diameter of a sparger (15 m-sized) varied at 1.5, 3.0, 5.0 and 8.0 cm, respectively. The highest root growth (191.9 g dry wt.) and the ginsenoside content (4.9 mg/g dry wt.) were obtained in the sparger diameter of 8.0 cm.
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