분자육종기법에 의해 선발된 형질전환 벼 계통의 작물학적 특성
Characteristics of Agronomy Traits to Transgenic Rice Selected by MolecularBreeding Method
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초록
This study was carried out to develop new cultivars using the T5 generation of transformed rice by PCR analysis with DNA marker in each generation (from T3 to T5). In the previous study, we successfully developed the transgenic rice plants over-expressing the Arabidopsis H+/Ca2+ antiporter CAX 1 (accession no. U57411) gene. The calcium concentration in brown rice of transgenic plants was higher than that of donor plants, Iipum, and was selected 3 lines out of 25 lines at cultured GMO field. The major agronomic traits such as culm length, panicle length and panicle number of 3 lines at transgenic plants (T5) were similar to wild type. Also these lines appeared to have disease resistance to rice blast, cold resistance as compared with donor types. The grain shape was similar to donor plant, however, the 1000 grain weight of brown rice was different from transgenic plants. These finding would be used for basic data of new variety registration.
Abstract
This study was carried out to develop new cultivars using the T5 generation of transformed rice by PCR analysis with DNA marker in each generation (from T3 to T5). In the previous study, we successfully developed the transgenic rice plants over-expressing the Arabidopsis H+/Ca2+ antiporter CAX 1 (accession no. U57411) gene. The calcium concentration in brown rice of transgenic plants was higher than that of donor plants, Iipum, and was selected 3 lines out of 25 lines at cultured GMO field. The major agronomic traits such as culm length, panicle length and panicle number of 3 lines at transgenic plants (T5) were similar to wild type. Also these lines appeared to have disease resistance to rice blast, cold resistance as compared with donor types. The grain shape was similar to donor plant, however, the 1000 grain weight of brown rice was different from transgenic plants. These finding would be used for basic data of new variety registration.
참고문헌 (29)
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