Identification of salt and drought inducible glutathione S-transferase genes of hybrid poplar
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Recent genome annotation revealed that Populustrichocarpa contains 81 glutathione S-transferase (GST)genes. GST genes play important and varying roles in plants,including conferring tolerance to various abiotic stresses. Little information is available on the relationship – if any –between drought/salt stresses and GSTs in woody plants. Inthis study, we screened the PatgGST genes in hybrid poplar(Populus alba × Populus tremula var. glandulosa) that werepredicted to confer drought tolerance based on our expressionanalysis of all members of the poplar GST superfamilyfollowing exposure to salt (NaCl) and drought (PEG) stresses,respectively. Exposure to the salt stress resulted in theinduction of eight PatgGST genes and down-regulation ofone PatgGST gene, and the level of induction/repression wasdifferent in leaf and stem tissues. In contrast, 16 PatgGSTgenes were induced following exposure to the drought (PEG)stress, and two were down-regulated. Taken together, weidentified seven PatgGSTs (PatgGSTU15, PatgGSTU18,PatgGSTU22, PatgGSTU27, PatgGSTU46, PatgGSTU51and PatgGSTU52) as putative drought tolerance genes basedon their induction by both salt and drought stresses.
Abstract
Recent genome annotation revealed that Populustrichocarpa contains 81 glutathione S-transferase (GST)genes. GST genes play important and varying roles in plants,including conferring tolerance to various abiotic stresses. Little information is available on the relationship – if any –between drought/salt stresses and GSTs in woody plants. Inthis study, we screened the PatgGST genes in hybrid poplar(Populus alba × Populus tremula var. glandulosa) that werepredicted to confer drought tolerance based on our expressionanalysis of all members of the poplar GST superfamilyfollowing exposure to salt (NaCl) and drought (PEG) stresses,respectively. Exposure to the salt stress resulted in theinduction of eight PatgGST genes and down-regulation ofone PatgGST gene, and the level of induction/repression wasdifferent in leaf and stem tissues. In contrast, 16 PatgGSTgenes were induced following exposure to the drought (PEG)stress, and two were down-regulated. Taken together, weidentified seven PatgGSTs (PatgGSTU15, PatgGSTU18,PatgGSTU22, PatgGSTU27, PatgGSTU46, PatgGSTU51and PatgGSTU52) as putative drought tolerance genes basedon their induction by both salt and drought stresses.
참고문헌 (26)
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