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Sodium hypochlorite treatment and light-emitting diode (LED) irradiation effect on in vitro germination of Oreorchis patens (Lindl.) Lindl

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In this study, we investigated the effects ofsodium hypochlorite (NaOCl) and red or blue light-emittingdiode (LED) light on embryo swelling and germination ofOreorchis patens (Lindl.) Lindl. A method for determiningthe swelling and protocorm formation in O. patens seedsthrough in vitro examination of immature seeds was established. NaOCl treatment of immature seeds was found to significantlyenhance the extent of embryo swelling and protocormformation in immature zygote embryos compared to those inthe untreated controls. Additionally, the effects of whitefluorescent light, and red and blue LED lights on embryoswelling and protocorm formation in in vitro cultured seedswere examined and compared to the conditions with orwithout NaOCl treatment. The most suitable light for embryoswelling and protocorm formation was the red LED light.

Abstract

In this study, we investigated the effects ofsodium hypochlorite (NaOCl) and red or blue light-emittingdiode (LED) light on embryo swelling and germination ofOreorchis patens (Lindl.) Lindl. A method for determiningthe swelling and protocorm formation in O. patens seedsthrough in vitro examination of immature seeds was established. NaOCl treatment of immature seeds was found to significantlyenhance the extent of embryo swelling and protocormformation in immature zygote embryos compared to those inthe untreated controls. Additionally, the effects of whitefluorescent light, and red and blue LED lights on embryoswelling and protocorm formation in in vitro cultured seedswere examined and compared to the conditions with orwithout NaOCl treatment. The most suitable light for embryoswelling and protocorm formation was the red LED light.

참고문헌 (31)

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