금새우난초(Calanthe striata f. sieboldii Decne. ex Regel)종자의 비공생 발아 및 신초증식
Asymbiotic germination and seedling growth of Calanthe striata f. sieboldii Decne. ex Regel
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초록
Calanthe striata f. sieboldii Decne. ex Regel is a terrestrial orchid with beautiful flowers arranged in racemose inflorescences. This species is threatened due to over-collection and loss of suitable habitats. Asymbiotic germination is useful in the conservation efforts to re-establish plants in the wild, and for commercial propagation. In this study, we investigate the effects of sodium hypochlorite (NaOCl), three types of culture media (Phytomax Orchid Maintenance – POM, Seed Germination Maintenance - SGM, and Murashige and Skoog 1962 - MS), and plant growth regulators on embryo swelling, protocorm formation, and embryo diameter of C. striata f. sieboldii. Treatment with 1% NaOCl for 30 min greatly enhanced embryo swelling (28.3%), embryo diameter (205.8 μm), and embryo protocorm formation (54.8%) compared to seeds without NaOCl treatment (embryo swelling 8.5%, embryo diameter 14.6 μm, and protocorm formation 13.4%) on POM medium. Protocorm formation on POM medium supplemented with 1.0 mg/L N6-benzyladenine (BA) (95.6%) was better than the control (54.5%). Additionally, the effects of activated charcoal (AC) and sucrose on seedling growth in in vitro culture were examined. The protocorm converted into healthy plants with well-developed shoot primordia on the POM medium with AC and sucrose. The most suitable conditions for seedling growth after 10 weeks of culture were the POM medium with AC or sucrose. These results show effective asymbiotic seed germination and in vitro seedling growth of C. striata f. sieboldii.
Abstract
Calanthe striata f. sieboldii Decne. ex Regel is a terrestrial orchid with beautiful flowers arranged in racemose inflorescences. This species is threatened due to over-collection and loss of suitable habitats. Asymbiotic germination is useful in the conservation efforts to re-establish plants in the wild, and for commercial propagation. In this study, we investigate the effects of sodium hypochlorite (NaOCl), three types of culture media (Phytomax Orchid Maintenance – POM, Seed Germination Maintenance - SGM, and Murashige and Skoog 1962 - MS), and plant growth regulators on embryo swelling, protocorm formation, and embryo diameter of C. striata f. sieboldii. Treatment with 1% NaOCl for 30 min greatly enhanced embryo swelling (28.3%), embryo diameter (205.8 μm), and embryo protocorm formation (54.8%) compared to seeds without NaOCl treatment (embryo swelling 8.5%, embryo diameter 14.6 μm, and protocorm formation 13.4%) on POM medium. Protocorm formation on POM medium supplemented with 1.0 mg/L N6-benzyladenine (BA) (95.6%) was better than the control (54.5%). Additionally, the effects of activated charcoal (AC) and sucrose on seedling growth in in vitro culture were examined. The protocorm converted into healthy plants with well-developed shoot primordia on the POM medium with AC and sucrose. The most suitable conditions for seedling growth after 10 weeks of culture were the POM medium with AC or sucrose. These results show effective asymbiotic seed germination and in vitro seedling growth of C. striata f. sieboldii.
참고문헌 (33)
- Bae KH, "Structural changes of seed coats and stimulation of in vitro germination of fully mature seeds of Cypripedium macranthos Swartz by NaOCl pre-treatment", Prog Ornament Plant, 10, pp.107~113 (2010)
- Bae KH, "Factors affecting fruit baring in natural habitate and in vitro culture of zygotic embryos of Cypripedium japonicum", Prog Ornament Plant, 11, pp.146~152 (2013)
- Bae KH, "In vitro germination and plantlet conversion from the culture of fully mature seeds of Cypripedium guttatum Swartz", Prog Ornament Plant, 9, pp.160~165 (2009)
- 배기화, "NaOCl 처리에 의한 한라천마(Gastrodia verrucosa Blume)와 애기천마 (Hetaeria sikokiana Tuyama)의 기내발아", Journal of Plant Biotechnology, 39(3), pp.163~168 (2012), 한국식물생명공학회[DOI]
- 배기화, "Sodium hypochlorite treatment and light-emitting diode (LED) irradiation effect on in vitro germination of Oreorchis patens (Lindl.) Lindl", Journal of Plant Biotechnology, 41(1), pp.44~49 (2014), 한국식물생명공학회
- Chung MY, "Effect of culture media on asymbiotic seed germination and those seedling growth of Calanthe discolor and Habenaria radiata", Kor J Plant Tiss Cult, 25, pp.189~194 (1988)
- Dumas E, "In vitro rooting of micropropagated shoots from juvenile and mature Pinus pinaster explants influence of activated charcoal", Plant Cell Tiss Org Cult, 40, pp.231~235 (1995)
- Eymar E, "Effect of activated charcoal and 6-benzyladenine on in vitro nitrogen uptake by Lagerstroemia indica", Plant Cell Tiss Org Cult, 63, pp.57~65 (2000)
- Gabriela F, "Exogenous sucrose can decrease in vitro photosynthesis but improve field survival and growth of coconut (Cocos nucifera L.) in vitro plantlets", In Vitro Cell Devel Biol, 41, pp.69~76 (2005)
- Gale S, "Calanthe arisanensis Orchidaceae", Curtis’s Bot. Mag, 24, pp.206~210 (2007)
- Godo T, "Germination of mature seeds of Calanthe tricarinata Lindl., an endangered terrestrial orchid, by asymbiotic culture in vitro", In Vitro Cell Dev Biol Plant, 46, pp.323~332 (2010)
- Hyun MR, "Studies on distributions and morphological characteristics of Calanthe discolor, C. sieboldii, and C. bicolor native to Cheju Province", Kor J Hort Sci Technol, 17, pp.498~500 (1999)
- Hyun MR, "Isozyme and randomly amplified polymorphic DNA (RAPD) analysis for genetic relationship among Calanthe discolor, C. sieboldii, and C. bicolor native to Cheju Island", Kor J Hort Sci Technol, 17, pp.141~143 (1999)
- Kano K, "The orchids. Seibundo-shinkosha, Tokyo Kim BC, Kim MH, Oh MY (1990) A taxonomic study on Calanthe in Cheju island - A comparative study on isozyme by electrophoresis", Kor J Plant Tax, 20, pp.53~64 (1972)
- Kim CH, "Asymbiotic germination of Korean native Calanthe species", Agric Res Bull Kyungpook Natl Univ, 15, pp.47~52 (1997)
- 의 종간교배에 의한 1대잡종 종자의 기내무균 발아, "한국자원식물학회지", 2008, 341~345, pp.한국자원식물학회
- Kim YS, "A taxonomic study on Calanthe in Korea", Kor J Plant Tax, 19, pp.273~287 (1989)
- 이은주, "自生 붓꽃 種子發芽 증진연구", 원예과학기술지, 20(4), pp.345~351 (2002), 한국원예학회
- 이긍주, "A Wide Array of Phenotypic Components and Their Contributions to Variations in Calanthe Orchid Landraces", Horticulture, Environment, and Biotechnology, 49(6), pp.418~426 (2008), 한국원예학회
- Lee JS, "Useful Plant Resources in Mt. Moak Sited Jeonlabuk-do, Korea", J Kor Flower Res Soc, 2, pp.55~72 (1993)
- 이진실, "한반도 야생란의 분포 및 보호 대상 식물", 식물분류학회지, 36(4), pp.335~360 (2006), 한국식물분류학회
- Lee K, "The wild orchids of Korea", pp.193~ (1980), Jungsung publisher
- Lee NS, "Illustrated flora of Korean Orchids", pp.208~ (2011), Ewha Womans University publisher
- Lee YN, "Flora of Korea" (2006), Gyohaksa
- Magdalena K, "Morphogenesis of Lilium martagon L. explants in callus culture", Acta Biol Craco Series Bot, 47, pp.65~73 (2005)
- Mathews VH, "In vitro multiplications of Vanda hybrids through tissue culture technique", Plant Sci Lett, 17, pp.383~389 (1980)
- Miyoshi K, "Ultrasonic treatment for enhancing seed germination of terrestrial orchid, Calanthe discolor, in asymbiotic culture", Scientia Horticulturae, 35, pp.127~130 (1988)
- Miyoshi K, "Phytohormone pre-treatment for the enhancement of seed germination and protocorm formation by the terrestrial orchid, Calanthe discolor (Orchidaceae), in asymbiotic culture", Scientia Horticulture, 63, pp.263~267 (1995)
- Miyoshi K, "Enhancement of seed germination and protocorm formation in Calanthe discolor (Orchidaceae) by NaOCl and poly phenol absorbent treatments", Plant Tiss Cult Lett, 12, pp.267~272 (1995)
- Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol Plant, 15, pp.473~479 (1962)
- Pan MJ, "The use of charcoal in in vitro culture-a review", Plant Growth Regul, 26, pp.155~163 (1998)
- Park SY, "In vitro seed germination of Calanthe sieboldii, an endangered orchid species", J Plant Biology, 43, pp.158~161 (2000)
- Takayama S, "Differentiation in Lilium bulbscales grown in vitro: Effect of various cultural conditions", Physi Planta, 46, pp.184~190 (1979)