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Asymbiotic seed germination and in vitro propagation condition in Calanthe discolor Lindl

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We investigated the effects of sodium hypochlorite (NaOCl) and culture medium on embryo swelling and germination of Calanthe discolor Lindl., and established a method for determining the swelling and protocorm formation of C. discolor seeds via in vitro examination of immature seeds. Treatment of immature seeds with NaOCl greatly enhanced the extent of embryo swelling and protocorm formation of immature zygote embryos compared to seeds without NaOCl treatment. The effects of the culture media were also evaluated with regard to embryo swelling and protocorm formation of in vitro cultured seeds with and without NaOCl treatment. Additionally, the effects of white fluorescent light and red and blue LEDs lights on seedling growth in in vitro culture were examined. The most suitable condition for seedling growth after 12 weeks of culture was the red LEDs light with POM medium. These results show effective asymbiotic germination and growth of C. discolor.

Abstract

We investigated the effects of sodium hypochlorite (NaOCl) and culture medium on embryo swelling and germination of Calanthe discolor Lindl., and established a method for determining the swelling and protocorm formation of C. discolor seeds via in vitro examination of immature seeds. Treatment of immature seeds with NaOCl greatly enhanced the extent of embryo swelling and protocorm formation of immature zygote embryos compared to seeds without NaOCl treatment. The effects of the culture media were also evaluated with regard to embryo swelling and protocorm formation of in vitro cultured seeds with and without NaOCl treatment. Additionally, the effects of white fluorescent light and red and blue LEDs lights on seedling growth in in vitro culture were examined. The most suitable condition for seedling growth after 12 weeks of culture was the red LEDs light with POM medium. These results show effective asymbiotic germination and growth of C. discolor.

참고문헌 (47)

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관련 논문AI추천

Sodium hypochlorite treatment and light-emitting diode (LED) irradiation effect on in vitro germination of Oreorchis patens (Lindl.) Lindl김수영, 배기화, 오경희한국식물생명공학회 · Journal of Plant Biotechnology2014 · 제41권 제1호금새우난초(Calanthe striata f. sieboldii Decne. ex Regel)종자의 비공생 발아 및 신초증식김수영, 배기화한국식물생명공학회 · Journal of Plant Biotechnology2015 · 제42권 제3호Effects of NaOCl treatment on in vitro germination of seeds of a rare endemic plant, Oreorchis coreana Finet배기화, 송관필, 송재모, Mi Hyun Lee, Myoung Suk Ko, Nam Young Kim한국식물생명공학회 · Journal of Plant Biotechnology2013 · 제40권 제1호새우난초(Calanthe discolor Lindl.)와 금새우난초[C. discolor for. sieboldii (Decne.) Ohwi]의 종간교배에 의한 1대잡종 종자의 기내무균 발아김광수, 김종선, 박종환한국자원식물학회 · 한국자원식물학회지2008 · 제21권 제4호NaOCl 처리 농도 및 시간에 따른 희귀식물 자란(Bletilla striata Rchb.f.)의 종자 발아 및 활력 연구김진기, 배기화, 송세규, 이하얀, 정영호한국식물생명공학회 · Journal of Plant Biotechnology2020 · 제47권 제1호Effects of altering medium strength and sucrose concentration on in vitro germination and seedling growth of Cypripedium macranthos Sw.남상영, 백기엽, 손성원, 이정관, 허윤선, 홍의연한국식물생명공학회 · Journal of Plant Biotechnology2016 · 제43권 제1호

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