한국 육성 벼 품종의 DNA profiling에 의한 유전적 다양성 분석 및 품종 판별
Discrimination of Korean rice varieties as revealed by DNA profiling and its relationship with genetic diversity
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
This study is to establish the varietal discrimination based on DNA profiling of different varieties of rice. We examined the genetic distance among Korean rice varieties using allele frequencies and a genetic diversity analysis with Simple Sequence Repeats (SSRs) markers. The analysis of the genetic diversity and genetic relationships of 243 Korean rice varieties was varied out using 20 SSRs markers. A total of 268 alleles were detected, ranging from 6 to 32, with an average of 13.45 alleles per locus, and and average of gene diversity (GD) of 0.556. Seven SSR markers were selected as key markers for discrimination among the Korean rice varieties. Concerning the results, 243 varieties (100%) were discriminated among by using acrylamide gel and fragment analyzer-based markers. In conclusion, this study provides useful basic data that can be utilized concerning Korean rice varieties breeding and development. In addition, we will have to manage and conserve as a valuable genetic resource, without losing the diversity of Korean rice varieties.
Abstract
This study is to establish the varietal discrimination based on DNA profiling of different varieties of rice. We examined the genetic distance among Korean rice varieties using allele frequencies and a genetic diversity analysis with Simple Sequence Repeats (SSRs) markers. The analysis of the genetic diversity and genetic relationships of 243 Korean rice varieties was varied out using 20 SSRs markers. A total of 268 alleles were detected, ranging from 6 to 32, with an average of 13.45 alleles per locus, and and average of gene diversity (GD) of 0.556. Seven SSR markers were selected as key markers for discrimination among the Korean rice varieties. Concerning the results, 243 varieties (100%) were discriminated among by using acrylamide gel and fragment analyzer-based markers. In conclusion, this study provides useful basic data that can be utilized concerning Korean rice varieties breeding and development. In addition, we will have to manage and conserve as a valuable genetic resource, without losing the diversity of Korean rice varieties.
참고문헌 (18)
- Gao L, "A SNP-based molecular barcode for characterization of common wheat", PLoS ONE, 11(3), pp.e0150947~ (2016)
- He C, "Development and characterization of simple sequence repeat (SSR) markers and their use in determining relationships among Lycopersicon esculentum cultivars", Theoretical and Applied Genetics, 106(2), pp.363~373 (2003)
- 홍지화, "Microsatellite 마커를 이용한 사과 품종 간 유전적 유연관계 분석", 생명과학회지, 23(6), pp.721~727 (2013), 한국생명과학회[DOI]
- 홍지화, "Simple Sequence Repeat (SSR) 마커를 이용한 장미 품종 식별", 한국육종학회지, 45(2), pp.96~103 (2013), 한국육종학회[DOI]
- 황지현, "분자마커 이용 여교잡 육종을 위한 토마토 유전자원 평가 및 SSR 마커 개발", 원예과학기술지, 30(5), pp.557~567 (2012), 한국원예학회[DOI]
- Hwang TY, "Genetic diversity of cultivated and wild soybeans including Japanese elite cultivars as revealed by length polymorphism of SSR markers", Breeding Science, 58, pp.315~323 (2008)
- 김율호, "STS-CAPS 마커 개발을 통한 110개 한국 콩 품종 판별", 한국육종학회지, 44(3), pp.258~272 (2012), 한국육종학회
- 장성진, "한국 콩 보급품종을 포함한 엘리트품종의 SSR마커에 의한 유전적 다양성과 품종판별", 한국작물학회지, 54(2), pp.231~240 (2009), 한국작물학회
- 권용삼, "Microsatellite 마커를 이용한 오이 유통품종 DNA Profile Data Base 구축", 원예과학기술지, 31(3), pp.344~351 (2013), 한국원예학회[DOI]
- 권용삼, "Simple sequence repeat (SSR) marker를 이용한 벼 품종 식별", 생명과학회지, 16(6), pp.1001~1005 (2006), 한국생명과학회
- Gi-Jun Kim, "Application of UPOV Data for the Analysis of Genetic Variation in Rose Cultivars", 원예과학기술지, 29(3), pp.240~246 (2011), 한국원예학회
- 김홍식, "한국 콩 육성품종의 SSR마커에 의한 유전적 다양성과 유연관계", 한국작물학회지, 51(3), pp.248~258 (2006), 한국작물학회
- Park SJ, "Genetic diversity and relationships of 7 rice cultivars in Korea", Journal of Agricultural, Life and Environmental Sciences, 23(2), pp.49~54 (2011)
- Rural Development Administration, "Standard methods for agricultural experimental experiments", ~ (2003)
- Singh RK, "Suitability of mapped sequence tagged microsatellite site markers for establishing distinctness, uniformity and stability in aromatic rice", Euphytica, 135, pp.135~143 (2004)
- Ming-Mao Sun, "SSR 마커에 의한 최근 육성 보급된 한국 벼 품종의 다양성과 품종 판별", 한국육종학회지, 41(2), pp.115~125 (2009), 한국육종학회
- Tian HL, "Development of maizeSNP3072, a highthroughput compatible SNP array, for DNA fingerprinting identification of Chinese maize varieties", Mol Breeding, 35, pp.136~ (2015)
- 윤웅한, "벼 glutelin 유전자 구조 및 발현특성분석", Journal of Plant Biotechnology, 38(2), pp.176~185 (2011), 한국식물생명공학회