핵심 Microsatellite 마커를 이용한 한국 콩 품종에 대한 Fingerprinting 분석
DNA fingerprinting analysis for soybean (Glycine max) varieties in Korea using a core set of microsatellite marker
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핵심 microsatellite 마커를 활용하여 우리나라에서 품종보호출원 및 등록된 콩 148 품종에 대한 DNA 프로파일 데이터베이스를 구축한 다음 유전적 유사도 분석을 통한품종 식별력 정도를 조사하였다. 콩 148품종을 120개의microsatellite 마커로 검정하고 밴드의 패턴이 명확하고다형성 정도가 높은 핵심 마커 16개의 대립유전자의 수는 6 ~ 28개로 나타났고 평균 대립유전자의 수는 12.75개였다. PIC 값은 0.753 ~ 0.951 사이에 분포하였고 평균값은 0.863으로 아주 높았다. 콩 148 품종에 대하여 Jaccard 방법에 따라 유전적 유사도를 설정한 다음 비가중 산술평균결합에 의해 집괴분석하여 계통도를 작성하였을 때, 콩의 품종 유형 및 품종 육성 계보에 따라 5개의 대그룹으로 나누어졌으며 모든 품종이 식별이 가능하였다. 본 연구에서 콩 품종식별용 핵심 microsatellite는 품종보호출원 품종의 구별성, 균일성, 안정성의 확인, 품종진위성과 관련된 종자분쟁, 종자 순도 관리에 매우 유용하게 활용될 수 있을 것이다.
Abstract
Microsatellites are one of the most suitable markers for identification of variety, as they have the capability to discriminate between narrow genetic variations. The polymorphism level between 120 microsatellite primer pairs and 148 soybean varieties was investigated through the fluorescence based automatic detection system. A set of 16 primer pairs showed highly reproducible polymorphism in these varieties. A total of 204 alleles were detected using the 16 microsatellite markers. The number of alleles per locus ranged from 6 to 28, with an average of 12.75 alleles per locus. The average polymorphism information content (PIC) was 0.86, ranging from 0.75 to 0.95. The unweighted pair group method using the arithmetic averages (UPGMA) cluster analysis for 148 varieties were divided into five distinctive groups, reflecting the varietal types and pedigree information. All the varieties were perfectly discriminated by marker genotypes. These markers may be useful to complement a morphological assessment of candidate varieties in the DUS (distinctness, uniformity and stability) test, intervening of seed disputes relating to variety authentication, and testing of genetic purity in soybean varieties.
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