타우린 처리에 의한 나리 기내 식물체 생산체계 최적화
Optimization of in vitro lily culture system with different treatments of taurine
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초록
Lilies as cut flowers are one of the most popular ornamental plants in South Korea. It is necessary to develop lily cultivars with high qualities. Therefore, highly efficient propagation systems are needed following release of elite cultivars. In this study, we used taurine treatment to improve the growth conditions including shoot and bulb formation, fresh weight gain, and reduction of rooting and browning. We experimentally evaluated the effect of taurine as a growth stimulator, at concentrations of 0, 2.5, 5, 10, 15 and 20 mg/l. The results showed that 20 mg of taurine enhanced shoot formation by 85% and increased fresh weight 5.5-fold, which was higher than the approximately four-fold increase in the control. In addition, multiple bulb formation rate was increased by 80% and rooting by 82% following exposure to 20 mg/l of taurine. The efficiency of taurine treatment was higher than that of control with 50% multiple bulb formation rate and 60% rooting rate. The browning was 10.6% at 2.5 mg/l of taurine when compared with 0.8% at 20 mg/l. Taurine showed a positive effect on the overall growth of lily plants in terms of increased fresh weight, shoot formation rate, rooting, and formation of multiple bulbs, indicating that taurine can be used as an alternative to amino acids or as an antioxidant such as citrate and vitamin C in plant tissue culture.
Abstract
Lilies as cut flowers are one of the most popular ornamental plants in South Korea. It is necessary to develop lily cultivars with high qualities. Therefore, highly efficient propagation systems are needed following release of elite cultivars. In this study, we used taurine treatment to improve the growth conditions including shoot and bulb formation, fresh weight gain, and reduction of rooting and browning. We experimentally evaluated the effect of taurine as a growth stimulator, at concentrations of 0, 2.5, 5, 10, 15 and 20 mg/l. The results showed that 20 mg of taurine enhanced shoot formation by 85% and increased fresh weight 5.5-fold, which was higher than the approximately four-fold increase in the control. In addition, multiple bulb formation rate was increased by 80% and rooting by 82% following exposure to 20 mg/l of taurine. The efficiency of taurine treatment was higher than that of control with 50% multiple bulb formation rate and 60% rooting rate. The browning was 10.6% at 2.5 mg/l of taurine when compared with 0.8% at 20 mg/l. Taurine showed a positive effect on the overall growth of lily plants in terms of increased fresh weight, shoot formation rate, rooting, and formation of multiple bulbs, indicating that taurine can be used as an alternative to amino acids or as an antioxidant such as citrate and vitamin C in plant tissue culture.
참고문헌 (26)
- Anthony JM, "The role of antioxidants for initiation of somatic embryos with Conostephium pendulum (Ericaceae)", Plant Cell Tiss organ Cult, 78(3), pp.247~252 (2004)
- Arnaldos TL, "Changes in phenol content during strawberry (Fragariaⅹananasa, cv. Chandler) callus culture", Physiol Plant, 113, pp.315~322 (2001)
- Druart PH, "Regulation of auxiliary branching in micropropagation of woody fruit species", Acta Hort, 227, pp.369~380 (1988)
- Ferreira BAL, "Metabolic response to salt stress in cell suspension cultures of sensitive and resistant citrus", J Hortic Sci Biotechnol, 81(6), pp.983~987 (2006)
- George EF, "Plant propagation by tissue culture. The background" (2008), Springer
- "World Checklists of Liliaceae. Royal Botanic Gardens" (2013)[URL]
- Han DS, "Regeneration of haploid plants from anther cultures of the Asiatic hybrid lily ‘Connecticut King’", Plant Cell Tiss Organ Cult, 47, pp.153~158 (1997)
- Hao LH, "Physiological effects of taurine on the growth of wheat (Triticum aestivum L.) seedlings Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Bao", Chin J Plant Physiol Mol Biol, 30(5), pp.595~598 (2004)
- Jeong JH, "Effect of scale position, chilling duration and IBA on bulblet formation in scale cuttings of Lilium hansonii", Flower Res J, 23, pp.33~41 (1998)
- Joshi SK, "In vitro propagation from axenic explants of Lilium oxypetalum (D. Don) Baker, an endemic bulbous plant of high altitude Himalaya", Acta Physiol Plant, 31, pp.833~838 (2009)
- 김종보, "유전자총 실험조건 최적화를 통한 형질전환 백합 식물체 생산", Journal of Plant Biotechnology, 44(1), pp.82~88 (2017), 한국식물생명공학회
- Ko WH, "Control of lethal browning of tissue culture plantlets of Cavendish banana cv. Formosana with ascorbic acid", Plant Tiss Organ Cult, 96(2), pp.137~141 (2009)
- Lee DH, "In vitro analysis of taurine as anti-stress agent in tomato (Solanum Lycopersicum)-preliminary study", Taurine, 9, pp.75~85 (2015), Springer Switzerland
- Liu J, "Regeneration and production of transgenic Lilium longiflorum via Agrobacterium tumefaciens", In Vitro Cell Dev Biol-Plant, 47, pp.348~356 (2011)
- Marinangeli PA, "Bulb quality and traumatic acid influence bulblet formation from scaling propagation of Lilium longiflorun", J Amer Soc Hort Sci, 128, pp.324~329 (1997)
- Statistics of floriculture cultivation in 2015, "Ministry of Food, Agriculture, Forestry and Fisheries", ~ (2015)
- Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue culture", Physiol Planta, 15, pp.473~497 (1962)
- Ozygit II, "Phenolic changes during in vitro organogenesis of cotton (Gossypium hirsutum L.) shoot tips", Afr J Biotech, 7(8), pp.1145~1150 (2008)
- Paek KY, "Plant Tissue Culture", pp.466~ (2001), Hyang-Moon publisher
- Roubelakis-Angelakis KA, "A new culture medium for in vitro rhizogenesis of grapevine (Vitis spp.)genotypes", HortSciences, 26(12), pp.1551~1553 (1991)
- 노희선, "Ascorbic acid, citric acid 및 AgNO3가 나리 기내식물체 생장촉진 및 갈변화 감소에 미치는 영향", Journal of Plant Biotechnology, 40(4), pp.224~230 (2013), 한국식물생명공학회
- Tang YP, "Callus induction and regeneration from in vitro cultured leaves, petioles and scales of Lilium leucanthum (Baker) Baker", Biotechnol Biotechnol Equip, 4, pp.2071~2076 (2010)
- Wang Y, "regeneration and Agrobacterium-mediated transformation of multiple lily cultivars", Plant Cell Tiss Organ Cult, 111, pp.113~122 (2012)
- 우진하, "오리엔탈 나리의 경정배양에 의한 lily symptomless virus 제거", 원예과학기술지, 22(3), pp.328~332 (2004), 한국원예학회
- 유용권, "오리엔탈 나리 ‘Siberia’ 인편삽시 인편 착생부위와 삽목조건에 따른 유공비닐봉지 내에서의 소인경 형성과 생장", 인간식물환경학회지, 9(1), pp.40~45 (2006), 인간식물환경학회
- Zoltan M, "Natural substances in tissue culture media of higher plants", Acta Biol Szeg, 55(1), pp.123~127 (2011)