Preconditioning for Cryopreservation of in vitro Grown Bulblets of Lily using Droplet-Vitrification
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
This study was conducted to improve and supplement the system of cryopreservation for adventitious bulbsinduced by tissue cultured bulb-scales of lily (Lilium spp.) cvs. ‘Milky way’. The explants, bulblets and bulb-scale-bulblets,were treated to low temperature (4℃) for 7 days prior to the pre-culture. The adventitious bulbs were pre-cultured inMurashige and Skoog (MS) liquid medium supplemented with sucrose (0.3 and 0.7M). The pre-cultured adventitious bulbswere treated to loading solution (LS1 or LS2, C4 or C6) containing 35% of PVS3 (LS1, C4) or 40% of PVS3 (LS2, C6) for40 min and exposed to dehydration solution (PVS3, B1) containing 50% glycerol and 50% sucrose for 60 min at 25℃. Theadventitious bulbs were moved onto droplets containing 3 µl PVS3 on sterilized aluminum foils, and then soaked into liquidnitrogen (LN) for 60 min. The result of highest regrowth rate as 65.7% was obtained in cold treatment (4℃), osmoprotectedwith LS1 solution, and cultured in PCM3 medium by using bulb-scale-bulblet for cryopreservation. This result shows thatdroplet-vitrification could be used as a promising method for long-term storage of lily genetic resource.
Abstract
This study was conducted to improve and supplement the system of cryopreservation for adventitious bulbsinduced by tissue cultured bulb-scales of lily (Lilium spp.) cvs. ‘Milky way’. The explants, bulblets and bulb-scale-bulblets,were treated to low temperature (4℃) for 7 days prior to the pre-culture. The adventitious bulbs were pre-cultured inMurashige and Skoog (MS) liquid medium supplemented with sucrose (0.3 and 0.7M). The pre-cultured adventitious bulbswere treated to loading solution (LS1 or LS2, C4 or C6) containing 35% of PVS3 (LS1, C4) or 40% of PVS3 (LS2, C6) for40 min and exposed to dehydration solution (PVS3, B1) containing 50% glycerol and 50% sucrose for 60 min at 25℃. Theadventitious bulbs were moved onto droplets containing 3 µl PVS3 on sterilized aluminum foils, and then soaked into liquidnitrogen (LN) for 60 min. The result of highest regrowth rate as 65.7% was obtained in cold treatment (4℃), osmoprotectedwith LS1 solution, and cultured in PCM3 medium by using bulb-scale-bulblet for cryopreservation. This result shows thatdroplet-vitrification could be used as a promising method for long-term storage of lily genetic resource.
참고문헌 (23)
- Benelli, C., "Recent advances in the cryopreservation of shoot-derived germplasm of economically important fruit trees of Actinidia, Malus, Olea, Prunus, Pyrus and Vitis", Biotechnol Adv., 31, pp.175~185 (2013)
- Benson, E. E., "Developmental competence and ploidy stability in plants regenerated from cryopreserved potato shoot tips", Cryo Lett, 17, pp.119~128 (1996)
- Bouman, H., "Cryopreservation of lily meristem", Acta Hortic, 266, pp.331~337 (1990)
- Bouman, H., "Cryopreservation of Lilium species and cultivars", Acta Hortic, 612, pp.147~154 (2003)
- Chen, X. L., "Cryopreservation of in vitro-grown apical meristems of Lilium by droplet-vitrification", S. Afr. J. Bot, 77, pp.397~403 (2011)
- Shambhu Prasad Dhital, "Elimination of Potato Viruses (PLRV and PVY) by Cryopreservation of In Vitro Grown Shoot Tips of Potato (Solanum tuberosum L.)", Horticulture, Environment, and Biotechnology, 50(3), pp.233~239 (2009), 한국원예학회
- Feng, C. H., "Cryopreservation of sweet potato (Ipomoea batatas) and its pathogen eradication by cryotherapy", Biotechnol. Adv, 29, pp.84~93 (2011)
- Kim, H. H., "Cryobanking of Korean allium germplasm collections: Results from a 10 year experiences", Cryo Lett, 33, pp.45~57 (2012)
- Kim, H. H., "Development of alternative plant vitrification solutions in droplet-vitrification procedures", Cryo Lett, 30, pp.320~334 (2009)
- Lambardi, M., "Cryopreservation of white poplar (Populus alba L.) by vitrification of in vitro-grown shoot tips", Plant Cell Rep, 19, pp.213~218 (2000)
- Li, B. Q., "Recovery patterns, histological observations and genetic integrity in Malus shoot tips cryopreserved using droplet-vitrification and encapsulation-dehydration pro-cedures", J. Biotechnol, 214, pp.182~191 (2015)
- Matsumoto, T., "Cryopreservation of in vitro-grown apical meristems of lily by vitrification", Plant Cell Tissue Organ. Cult, 41, pp.237~241 (1995)
- Rall, W. F., "Cryopreservation of mouse embryo at-196℃ by vitrification", Nature, 313, pp.573~575 (1985)
- Reed, B. M, "Implementing cryopreservation for long-term germplasm preservation in vegetatively propagated species", Biotechnology in Agriculture and Forestry, Cryopreservation of Plant Germplasm, 50, pp.22~33 (2002)
- Sakai, A., "Plant Cryopreservation: A Practical Guide", pp.33~58 (2008), Springer LLC
- Sant, R., "Cryopreservation of shoot-tips by droplet vitrification applicable to all taro (Colocasia esculenta var. esculenta) accessions", Plant Cell Tiss. Org. Cult, 92, pp.107~111 (2008)
- Tahtamouni, R. W., "Preservation at low temperature and cryopreservation in wild pear(Pyrus syriaca)", Adv. Hort. Sci, 13, pp.156~160 (1999)
- Wang, L. Y., "An efficient droplet vitrification cryopreservation for valuable blueberry germplasm", Sci. Hortic, 219, pp.60~69 (2017)
- Wang, Q. C., "Efficient elimination of sweet potato little leaf phytoplasma from sweet potato by cryotherapy of shoot tips", Plant Pathol, 57, pp.338~347 (2008)
- Wang, Q. C., "Elimination of grapevine virus A (GVA) by cryopreservation of in vitro-grown shoot tips of Vitis vinifera L", Plant Sci, 165, pp.321~327 (2003)
- Yamamoto, S., "Cryopreservation of in vitro-grown shoot tips of strawberry by the vitrification method using aluminum cryo-plates", Plant Gen. Res, 10, pp.14~19 (2012)
- 이정윤, "Efficient Cryopreservation of Lilium spp. Shoot Tips using Droplet-vitrification", Plant Breeding and Biotechnology, 1(2), pp.131~136 (2013), 한국육종학회[DOI]
- 이정윤, "Cryopreservation of Citrus limon (L.) Burm. F Shoot Tips", 한국자원식물학회지, 31(6), pp.684~694 (2018), 한국자원식물학회[DOI]