멸종위기종인 솔나리(Lilium cernum Komarvo.)의 인편 유래 캘러스 유도 및 기내 식물체 재분화
Plant regeneration through the callus culture induced from bulb scales of an endangered species Lilium cernum Komarvo
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초록
Lilium cernum Komarvo. is an important endangered plant belonging to the family Liliaceae. A method was developed for the rapid micropropagation of L. cernum through plant regeneration from bulb scales explant-derived calli. The bulb scales segments were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.5, 1.0, 3.0 mg․L-1 kinetin and 0, 0.1, 0.5, 1.0 mg․L-1 NAA or 2,4-D for callus induction. In media with 0.5∼3.0 mg․L-1 kinetin and 0.1∼1.0 mg․L-1 NAA and 2,4-D, 95∼100% of explants produced callus. They were then transferred to MS medium supplemented with various concentrations of NAA (0, 0.01, 0.05 and 1.0 mg․L-1) in combination with BA (0, 1.0 and 2.0 mg․L-1) for bulbet formation. Bulbet induction (78%), weight (468 mg) and size (15.5 mm) were obtained the highest on MS medium containing 2.0 mg․L-1 BA and 1.0 m g․L-1 NAA. In vitro frequency of plant regeneration was not significantly treated in strength of MS and sucrose concentration. Chlorophyll contents in 1/2MS with 50 g․L-1 sucrose treatments were higher than those in control and another treatment. This in vitro propagation protocol will be useful for conservation and mass propagation of this endangered plant.
Abstract
Lilium cernum Komarvo. is an important endangered plant belonging to the family Liliaceae. A method was developed for the rapid micropropagation of L. cernum through plant regeneration from bulb scales explant-derived calli. The bulb scales segments were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.5, 1.0, 3.0 mg․L-1 kinetin and 0, 0.1, 0.5, 1.0 mg․L-1 NAA or 2,4-D for callus induction. In media with 0.5∼3.0 mg․L-1 kinetin and 0.1∼1.0 mg․L-1 NAA and 2,4-D, 95∼100% of explants produced callus. They were then transferred to MS medium supplemented with various concentrations of NAA (0, 0.01, 0.05 and 1.0 mg․L-1) in combination with BA (0, 1.0 and 2.0 mg․L-1) for bulbet formation. Bulbet induction (78%), weight (468 mg) and size (15.5 mm) were obtained the highest on MS medium containing 2.0 mg․L-1 BA and 1.0 m g․L-1 NAA. In vitro frequency of plant regeneration was not significantly treated in strength of MS and sucrose concentration. Chlorophyll contents in 1/2MS with 50 g․L-1 sucrose treatments were higher than those in control and another treatment. This in vitro propagation protocol will be useful for conservation and mass propagation of this endangered plant.
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