Ascorbic acid, citric acid 및 AgNO3가 나리 기내식물체 생장촉진 및 갈변화 감소에 미치는 영향
Effects of ascorbic acid, citric acid and silver nitrate on the growth of in vitro lily plantlets and reduction of browning
1
조회
0
다운로드
6
인용
0
참고문헌
키워드
초록
Lily is an important cut-flower in Korea and world as well due to it’s a variety of flower colors and various sizes of flowers. To develop elite lily cultivars, conventional breeding techniques have been used so far. However, an introduction of tissue culture system in mass propagation of bulbs and regeneration of shoots with a high efficiency is prerequisite at this moment. Especially, growth of bulbs and shoots as well as reduction of browning is critical factor to proliferate bulbs with shoots of superior lines or cultivar in lily. For this purpose, we tried to test whether ascorbic acid, citric acid and silver nitrate in medium to facilitate growth of bulbs and shoots as well as reduction of browning of bulb scales in lily. As a result, ascorbic acid and silver nitrate showed no significant differences compared to control plants in the growth of bulbs and shoots. When bulb scales were treated with 150 mg․l-1 of citric acid, formations of shoots and bulbs showed best result. While, bulb scale treated with 100 mg․l-1 of citric acid showed the growth of shoot and root as well as increasing of fresh weight compared to other treatments. Regarding the reduction of browning, 150 mg․l-1 of ascorbic acid showed the best result with the less than 2%. Although more experiments with several commercial varieties are needed in the future to establish mass propagation of bulbs of lily, results obtained in this study are supposed to provide the basic knowledge and contribution in tissue culture system of lily.
Abstract
Lily is an important cut-flower in Korea and world as well due to it’s a variety of flower colors and various sizes of flowers. To develop elite lily cultivars, conventional breeding techniques have been used so far. However, an introduction of tissue culture system in mass propagation of bulbs and regeneration of shoots with a high efficiency is prerequisite at this moment. Especially, growth of bulbs and shoots as well as reduction of browning is critical factor to proliferate bulbs with shoots of superior lines or cultivar in lily. For this purpose, we tried to test whether ascorbic acid, citric acid and silver nitrate in medium to facilitate growth of bulbs and shoots as well as reduction of browning of bulb scales in lily. As a result, ascorbic acid and silver nitrate showed no significant differences compared to control plants in the growth of bulbs and shoots. When bulb scales were treated with 150 mg․l-1 of citric acid, formations of shoots and bulbs showed best result. While, bulb scale treated with 100 mg․l-1 of citric acid showed the growth of shoot and root as well as increasing of fresh weight compared to other treatments. Regarding the reduction of browning, 150 mg․l-1 of ascorbic acid showed the best result with the less than 2%. Although more experiments with several commercial varieties are needed in the future to establish mass propagation of bulbs of lily, results obtained in this study are supposed to provide the basic knowledge and contribution in tissue culture system of lily.
참고문헌 (36)
- Abdelwahd R, "Use of an adsorbent and antioxidants to reduce the effects of leached phenolics in in vitro plantlet regeneration of faba bean L", Afri J Biotec, 7(8), pp.997~1002 (2008)
- Al-Khayri JM, "Silver nitrate and 2-isopentyladenine promote somatic embryogenesis in date palm (Phoenix dactylifera L.)", Scientia Hortic, 89(4), pp.291~298 (2001)
- Anthony JM, "The role of antioxidants for initiation of somatic embryos with Conostephium pendulum (Ericaceae)", Plant Cell Tiss Org Cult, 78(3), pp.247~252 (2004)
- Arnaldos TL, "Changes in phenol content during strawberry (Fragaria x ananasa, cv. Chandler) callus culture", Physiol Plant, 113, pp.315~322 (2001)
- Beyer EM Jr, "A plant inhibitor of ethylen action on plants", Plnat Physiol, 58, pp.268~271 (1976)
- Bhatia P, "Improving the quality of in vitro culture shoots of tomato", Biotechnology, 7(2), pp.188~193 (2008)
- Chi GL, "Ethylene inhibitors enhanced de novo shoot regeneration from cotyledons of Brassica campestris ssp. Chinensis (Chinese cabbage) in vitro", Plant Sci, 64(2), pp.243~250 (1989)
- Chraibi BK, "Stimulation of shoot regeneration form cotyledons of Helianthus annuus by the ethylene inhibitor, silver and cobalt", Plant Cell Rep, 10, pp.204~207 (1991)
- Enríquez-Obregón, GA, "Agrobacteriummediated Japonica rice transformation: a procedure assisted by an antinecrotic treatment", Plant Cell Tiss Org Cult, 59(3), pp.159~168 (1999)
- Hatanaka T, "The role of ethylene in somatic embryogenesis from leaf discs of Coffea canephora", Plant Sci, 107(2), pp.199~204 (1995)
- Hyde CL, "Silver nitrate promotes shoot development and plant regeneration of chile pepper (Capsicum annuum L.) via organogenesis", In Vitro Cell Dev Biol-Plant, 32(2), pp.72~80 (1996)
- Khalid M, "Stimulation of shoot regeneration from cotyledons of Helianthus annuus by the ethylene inhibitors, silver and cobalt", Plant Cell Rep, 10(4), pp.204~207 (1991)
- Kim TJ, "Wild flowers of Korean" (1993), Kyohakaksa
- Ko WH, "Control of lethal browning of tissue culture plantlets of Cavendish banana cv. Formosana with ascorbic acid", Plant Cell Tiss Org Cult, 96(2), pp.137~141 (2009)
- Larue TAG, "Ethylene production by plant cellculture; Variation in during growing cycle and bioassays with tobacco tissue cultures", Physiol Plant, 15, pp.437~497 (1971)
- Lee HS, "Enhancemnet of shoot regeneration by ethylene inhobitors from cotyledon explant of Brassica camperstris L. spp. Pekinensis", Kor J Plant Tiss Cult, 22(5), pp.267~271 (1995)
- Mante S, "Propagation of Musa textilis Nee plants from apical meristem slices in vitro", Plant Cell Tiss Org Cult, 2(2), pp.151~159 (1983)
- Marton L, "Facile transformation of Arabidopsis", Plant Cell Rep, 10, pp.235~239 (1991)
- Mederos-Molina S, "Elimination of browning exudate and in vitro development of shoots in Pistacia vera L. cv. mateur and Pistacia atlantica Desf. Culture", Acta Soc Bot Pol, 68(1), pp.21~24 (1999)
- "Ministry of Food, Agriculture", Statistics of floriculture cultivation in 2011, ~ (2012)
- Misra P, "Clonal propagation of Rosa clinophylla Thory. through axillary bud culture", Scientia Hortic, 119(2), pp.212~216 (2009)
- Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol Plant, 15, pp.473~497 (1962)
- Ozyigit II, "Phenolic changes during in vitro organigenesis of cotten (Gossypium hirsutum L.) shot tips", Afr J Biotech, 7(8), pp.1145~1150 (2008)
- Ozyigit II, "Relation between explant age, total phenols and regeneration response of tissue cultured cotton (Gossypium hirsutum L.)", Afr J Biotechnol, 6(1), pp.3~8 (2007)
- Paek KY, "Plant tissue culture techniques", pp.466~ (2001), Hyang-moon Publisher
- Panaia M, "Micropropagation of the critically endangered Western Australian species, Symonanthus bancroftii (F. Muell.) L. Haegi (Solanaceae)", Plant Cell Tiss Org Cult, 63(1), pp.23~29 (2000)
- In Sook Park, "목자 선단부와 캘루스 배양에 의한 바이러스 감염 글라디올러스로부터의 바이러스 제거", Horticulture, Environment, and Biotechnology, 43(5), pp.531~535 (2002), 한국원예학회
- 박경일, "캘러스 배양에 의한 오리엔탈 나리의 LSV 무병주 획득", Horticulture, Environment, and Biotechnology, 44(5), pp.786~789 (2003), 한국원예학회
- Purnhauser L, "Stimulation of shoot regeneration in Triticum aestivum and Nicotiana plumbaginifolia Viv tissue cultures using the ethylene inhibitor AgNO3", Plant Cell Rep, 6(1), pp.1~4 (1987)
- 서석철, "토마토 재분화 효율 향상 및 엽록체 형질전환 조건", Journal of Plant Biotechnology, 37(1), pp.77~83 (2010), 한국식물생명공학회
- Roubelakis-Angelakis KA, "A new culture medium for in vitro rhizogenesis of grapevine (Vitis spp.) genotypes", HortScience, 26(12), pp.1551~1553 (1991)
- 서진나, "솔나리의 인편 재분화에 미치는 식물생장조절제 효과", 농업생명과학연구, 43(6), pp.29~33 (2009), 농업생명과학연구원
- Takasaki T, "Effect of silver nitrate on shoot regeneration and Agrobacterium-mediated transformation of turnip (Brassica rapa L. var. rapifera)", Plant Biotechnol J, 21(3), pp.225~228 (2004)
- 우진하, "오리엔탈 나리의 경정배양에 의한 lily symptomless virus 제거", 원예과학기술지, 22(3), pp.328~332 (2004), 한국원예학회
- 우진하, "오리엔탈나리 'Casa Blanca'에서 조직배양과 열처리에 의한 Lily Symptomless Virus의 제거", 화훼연구, 13(4), pp.341~346 (2005), 한국화훼학회
- 유용권, "오리엔탈 나리 ‘Siberia’ 인편삽시 인편 착생부위와 삽목조건에 따른 유공비닐봉지 내에서의 소인경 형성과 생장", 한국인간식물환경학회지, 9(1), pp.40~45 (2006), 한국인간식물환경학회