병풀의 추출용매에 따른 항균, 항염증 활성 및 피부 미백효능 등의 분석
Analysis of antibacterial, anti-inflammatory, and skin-whitening effect of Centella asiatica (L.) Urban
2
조회
0
다운로드
17
인용
0
참고문헌
키워드
초록
The imports of Centella asiatica L. Urban are increasing year-by-year due to the fact that its extract is a raw material used for skin wounds and in cosmetics. However, studies on the cultivation and identification of native C. asiatica species in Korea have been extremely rare. Therefore, this study was conducted in order to investigate the physiological and functional activity of Korean native C. asiatica plant cultivated in Hapcheon, Gyeongsangnam-do, Korea. As a result, the highest antibacterial and anti-inflammatory activities were examined with methanol extract while skin-whitening and wrinkle improvement were examined with water extract. Seven bacterium and one fungus were treated with 50% methanol extracts of C. asiatica and most of the bacterium showed similar or low levels of antibacterial activity compared to the control group of Omiza (Schisandra chinensis) extract, except for Streptococcus pyogenes, which showed higher antimicrobial activity than that of Omiza extract. However, neither C. asiatica and Omiza extracts showed antimicrobial activity against the fungus, C. albicans. The results of anti-inflammatory effect analyses with Raw 264.7 cells confirmed that the treatment of methanol extract reduced the level of NO by 50% or more compared to the control group. In addition, the water extract showed the highest reduction of melanin content of up to 20% more than the control group when examined with B16F10 cell line, indicating a significant skin-whitening effect. Furthermore, we were able to show the significant skin wrinkle improvement caused by C. asiatica extract with NHDF cell as an indicator, but strong cytotoxicity was also observed, suggesting that further studies are necessary.
Abstract
The imports of Centella asiatica L. Urban are increasing year-by-year due to the fact that its extract is a raw material used for skin wounds and in cosmetics. However, studies on the cultivation and identification of native C. asiatica species in Korea have been extremely rare. Therefore, this study was conducted in order to investigate the physiological and functional activity of Korean native C. asiatica plant cultivated in Hapcheon, Gyeongsangnam-do, Korea. As a result, the highest antibacterial and anti-inflammatory activities were examined with methanol extract while skin-whitening and wrinkle improvement were examined with water extract. Seven bacterium and one fungus were treated with 50% methanol extracts of C. asiatica and most of the bacterium showed similar or low levels of antibacterial activity compared to the control group of Omiza (Schisandra chinensis) extract, except for Streptococcus pyogenes, which showed higher antimicrobial activity than that of Omiza extract. However, neither C. asiatica and Omiza extracts showed antimicrobial activity against the fungus, C. albicans. The results of anti-inflammatory effect analyses with Raw 264.7 cells confirmed that the treatment of methanol extract reduced the level of NO by 50% or more compared to the control group. In addition, the water extract showed the highest reduction of melanin content of up to 20% more than the control group when examined with B16F10 cell line, indicating a significant skin-whitening effect. Furthermore, we were able to show the significant skin wrinkle improvement caused by C. asiatica extract with NHDF cell as an indicator, but strong cytotoxicity was also observed, suggesting that further studies are necessary.
참고문헌 (32)
- Ariffin F, "Antioxidant capacity and phenolic composition of fermented Centella asiatica herbal teas", J Sci Food Agric, 91, pp.2731~2739 (2011)[DOI]
- Arumugam T, "Phytochemical screening and antibacterial activity of leaf and callus extracts of Centella asiatica", Bangladesh J Pharmacol, 6, pp.55~60 (2011)
- Babykutty S, "Apoptosis induction of Centella asiatica on human breast cancer cells", Afr J Trad CAM, 6, pp.9~16 (2009)
- Bibi Y, "Regeneration of Centella asiatica plants from non- embryogenic cell lines and evaluation of antibacterial and antifungal properties of regenerated calli and plants", J Biol Eng, 5, pp.13~21 (2011)[DOI]
- Bylka W, "Centella asiatica in cosmetology", Postep Derm Alergol, 1, pp.46~49 (2013)[DOI]
- Bylka W, "Centella asiatica in dermatology: an overview", Phytother Res, 28, pp.1117~1124 (2014)[DOI]
- Ceremuga TE, "Evaluation of the anxiolytic and antidepressant effects of asiatic acid, a compound from Gotu Kola or Centella asiatica, in the Male Sprague Dawley Rat", AANA J, 83, pp.91~98 (2015)
- 최찬헌, "미강·황칠 발효추출물이 B16F10 세포의 미백에 미치는 효과", 동의생리병리학회지, 30(5), pp.301~307 (2016), 한의병리학회[DOI]
- Flora SJS, "Beneficial effects of Centella asiatica aqueous extract against arsenic-induced oxidative stress and essential metal status in rats", Phytother Res, 21, pp.980~988 (2007)[DOI]
- 하지혜, "병풀 추출물의 식용 나노입자화를 통한 면역 활성 증진", 한국약용작물학회지, 17(4), pp.257~265 (2009), 한국약용작물학회
- Hashim P, "Triterpene composition and bioactivities of Centella asiatica", Molecules, 16, pp.1310~1322 (2011)[DOI]
- Idris FN, "Antimicrobial activity of Centella asiatica on Aspergillus niger and Bacillus Subtilis", Chem Engin Trans, 56, pp.1381~1386 (2017)
- 조춘희, "병풀(Centella asiatica ) 추출물을 이용한 20~50대 여성의 두피 홍반개선 효과", 아시안뷰티화장품학술지, 12(6), pp.921~927 (2014), 한국피부과학연구원
- 길영숙, "병풀 지상부 조직에서 시기별 triterpene glycoside 함량 및 항산화활성 분석", 생명과학회지, 28(8), pp.917~922 (2018), 한국생명과학회[DOI]
- Kim HP, "Effects of titrated extract of Centella asiatica and epidermal growth factor on the proliferation of human epidermal keratinocyte", J Appl Pharmacol, 3, pp.80~84 (1995)
- 김옥태, "병풀 잎에서 triterpene glycosides의 시기별 함량변화", 한국약용작물학회지, 10(5), pp.375~378 (2002), 한국약용작물학회
- 김영주, "Centella asiatica의 추출조건에 따른 항산화 및 콜라겐 합성 효능", 한국미용학회지, 16(3), pp.829~834 (2010), 한국미용학회
- Krishnamurthy R, "Asiatic acid, a pentacyclic triterpene from Centella asiatica, is neuroprotective in a mouse model of focal cerebral ischemia", J Neurosci Res, 15, pp.2541~2550 (2009)
- Kumar A, "Neuroprotective effects of Centella asiatica against intracerebroventricular colchicine-induced cognitive impairment and oxidative stress", International Journal of Alzheimer's Disease, 2009, pp.1~8 (2009)[DOI]
- 권민철, "병풀의 초음파 추출 시 용매에 따른 면역활성 증진 효과", 한국약용작물학회지, 16(5), pp.294~300 (2008), 한국약용작물학회
- Lee JY, "Isolation of Antimicrobial Substance from Schizandra chinensis Baillon and Antimicrobial Effect", Korean J Food Sci Technol, 33, pp.389~394 (2001)
- Lalitha V, "Antibacterial and antifungal activity of aqueous extract of Centella asiatica L. against some important fungal and bacterial species", World J Pharm Pharmacue Scie, 2, pp.4744~4752 (2013)
- Mato L, "Centella asiatica improves physical performance and health-related quality of life in healthy elderly volunteer", Evidence-Based Complementary and Alternative Medicine, 2011, pp.1~7 (2011)[DOI]
- Orhan IE, "Centella asiatica (L.) Urban: From traditional medicine to modern medicine with neuroprotective potential", Evidence-Based Complementary and Alternative Medicine, 2012, pp.1~8 (2012)
- Pittella F, "Antioxidant and cytotoxic activities of Centella asiatica (L) Urb.", Int J Mol Sci, 10, pp.3713~3721 (2009)[DOI]
- Rao MK, "Treatment with Centalla asiatica (Linn) fresh leaf extract enhances learning ability and memory retention power in rats", Neurosciences, 12, pp.236~241 (2007)
- Schaneberg BT, "An improved HPLC method for quantitive determination of six triterpenes in Centella asiatica extracts and commercial products", Pharmazie, 58, pp.381~384 (2003)
- 서기석, "Asiaticoside의 입술 주름 개선 효과", 고신대학교 의과대학 학술지, 22(1), pp.220~231 (2007), 고신대학교 의과대학 학술지
- Taemchuay D, "Antibacterial activity of crude extracts of Centella asiatica against Staphylococcus aureus in bovine", Mastitis-Kasetsart Veterinarians, 19, pp.119~128 (2009)
- Verma RK, "Reverse-phase high performance liquid chromatography if asiaticoseide in Centella asiatica", Phytochem Anal, 10, pp.191~193 (1999)[DOI]
- 유영아, "Antimicrobial Activities of Omija Extracts Against Bacillus cereus and Escherchia coli", Journal of Bacteriology and Virology, 48(1), pp.31~36 (2018), 대한미생물학회[DOI]
- Zaidan MR, "In vitro screening of five local medicinal plants for antibacterial activity using disc diffusion method", Trop Biomed, 22, pp.165~170 (2005)