초음파 병행을 통한 병풀의 미백 및 자외선 차단 활성 증진 효과
Enhancement of Skin-Whitening and UV-Protective Effects of Centella asiatica L. Urban by Utrasonification Process
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초록
Enhancement effect of ultrasonification process on UV-protection and skin-whitening activities using Centella asiatica L. Urban extract was investigated. Cytotoxicity of the extracts measured on human skin fibroblast cells, CCD-986sk,and then, ultrasonification associated extracts showed 5~9% lower cytotoxicity then normal crude extracts on 1.0 ㎎/㎖ of highest sample concentration. The associated extrats showed highest inhibition activity of hyaluronidase on 1.0 ㎎/㎖ of concentration as 54.2%. Also, the associated extract reduced expression of MMP-1 on UV-irradiated CCD-986sk cells down to 100.2% from 136.1%, and revealed high inhibitory potency on tyrosinase as 74.6% by adding 1.0 ㎎/㎖ of concentration. Ultrasonification associated extract showed strong inhibition effect of melanin production on Clone M-3 cells as 84.2% by adding 1.0 ㎎/㎖ of concentration. From the preliminary observations, we considered that the extracts from C. asiatica could be potent natural materials for skin-whitening and anti-aging agent, and could enhance the activities by ultrasonification process.
Abstract
Enhancement effect of ultrasonification process on UV-protection and skin-whitening activities using Centella asiatica L. Urban extract was investigated. Cytotoxicity of the extracts measured on human skin fibroblast cells, CCD-986sk,and then, ultrasonification associated extracts showed 5~9% lower cytotoxicity then normal crude extracts on 1.0 ㎎/㎖ of highest sample concentration. The associated extrats showed highest inhibition activity of hyaluronidase on 1.0 ㎎/㎖ of concentration as 54.2%. Also, the associated extract reduced expression of MMP-1 on UV-irradiated CCD-986sk cells down to 100.2% from 136.1%, and revealed high inhibitory potency on tyrosinase as 74.6% by adding 1.0 ㎎/㎖ of concentration. Ultrasonification associated extract showed strong inhibition effect of melanin production on Clone M-3 cells as 84.2% by adding 1.0 ㎎/㎖ of concentration. From the preliminary observations, we considered that the extracts from C. asiatica could be potent natural materials for skin-whitening and anti-aging agent, and could enhance the activities by ultrasonification process.
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