병풀 부위별 기능성분 및 생리활성 분석
Functional components and physiological activity in different parts of Centella asiatica
1
조회
0
다운로드
6
인용
0
참고문헌
키워드
초록
We investigated the antioxidant and anti-inflammatory activities in the leaf, stem, and flower of Centella asiatica (CA). CA is widely used in the medical and cosmetic industries. The leaf contained the highest total polyphenol content (236.88 mg%) and antioxidant activity when compared to the stem and flower. The total triterpenoid content was 60.65 mg/g, 54.20 mg/g, and 12.50 mg/g (leaf, flower, and stem, respectively), indicating the triterpenoids in the stem were approximately 4 to 5 times lower than the CA leaf or flower. The cell viability evaluation using RAW 264.7 and HaCaT cells revealed no significant levels of cytotoxicity (≤ 100 μg/mL). The anti-inflammatory activity assessed using RAW 264.7 cells revealed the flower had higher activity than the leaf and stem, with NO (88.3 % - inhibitory rate), TNF-α (64.8 %), IL-6 (65.7 %), and MCP-1 (28.9 %) inhibitory activity. An analysis of the inflammatory inhibition activity on the skin using HaCaT cells revealed the flower possessed a potent activity level of IL-6 (72.0 %) and IL-8 (89.4 %) inhibition. Finally, the flower and leaf had potent physiological effects and functional components. We expect that the leaf and flowers can be used as functional materials, such as antioxidant and anti-inflammatory activities.
Abstract
We investigated the antioxidant and anti-inflammatory activities in the leaf, stem, and flower of Centella asiatica (CA). CA is widely used in the medical and cosmetic industries. The leaf contained the highest total polyphenol content (236.88 mg%) and antioxidant activity when compared to the stem and flower. The total triterpenoid content was 60.65 mg/g, 54.20 mg/g, and 12.50 mg/g (leaf, flower, and stem, respectively), indicating the triterpenoids in the stem were approximately 4 to 5 times lower than the CA leaf or flower. The cell viability evaluation using RAW 264.7 and HaCaT cells revealed no significant levels of cytotoxicity (≤ 100 μg/mL). The anti-inflammatory activity assessed using RAW 264.7 cells revealed the flower had higher activity than the leaf and stem, with NO (88.3 % - inhibitory rate), TNF-α (64.8 %), IL-6 (65.7 %), and MCP-1 (28.9 %) inhibitory activity. An analysis of the inflammatory inhibition activity on the skin using HaCaT cells revealed the flower possessed a potent activity level of IL-6 (72.0 %) and IL-8 (89.4 %) inhibition. Finally, the flower and leaf had potent physiological effects and functional components. We expect that the leaf and flowers can be used as functional materials, such as antioxidant and anti-inflammatory activities.
참고문헌 (33)
- Bylka W, "Centella asiatica in cosmetology", Postep Derm Alergol, 1, pp.46~49 (2013)
- Bylka W, "Centella asiatica in dermatology : An overview", Phytother Res, 28, pp.1117~1124 (2014)
- Chen YC, "Inhibition of nitric oxide synthase inhibitors and lipopolysaccharide induced inducible NOS and cyclooxygenase-2 gene expressions by rutin, quercetin, and quercetin pentaacetate in RAW 264.7 macrophages", J Cell Biochem, 82, pp.537~548 (2001)
- 최장남, "재배조건 및 관수방법에 따른 병풀 (Centella asiatica) 계통의 생육, Asiaticoside 함량과 항산화활성", 한국약용작물학회지, 28(4), pp.254~259 (2020), 한국약용작물학회[DOI]
- 최장남, "병풀의 생육 특성, Asiaticoside 함량 및 항산화 활성 비교", 한국자원식물학회지, 34(1), pp.44~51 (2021), 한국자원식물학회
- 최용민, "국내 시판되는 일부 다류 제품의 항산화 효과", 한국식품영양과학회지, 32(5), pp.723~727 (2003), 한국식품영양과학회
- 정해정, "구절초의 부위별 메탄올 추출물의 항산화활성", 한국식품저장유통학회지, 18(5), pp.739~745 (2011), 한국식품저장유통학회
- 엄현주, "건조방법에 따른 충주산 병풀의 영양성분과 생리활성", 한국식품영양학회지, 34(2), pp.165~173 (2021), 한국식품영양학회[DOI]
- 구영민, "병풀의 추출용매에 따른 항균, 항염증 활성 및 피부 미백효능 등의 분석", Journal of Plant Biotechnology, 45(2), pp.117~124 (2018), 한국식물생명공학회
- 하지혜, "병풀 추출물의 식용 나노입자화를 통한 면역 활성 증진", 한국약용작물학회지, 17(4), pp.257~265 (2009), 한국약용작물학회
- Ha Y, "Pyropia yezoensis extract suppresses IFN-gamma- and TNF-alpha-induced proinflammatory chemokine production in HaCaT cells via the downregulation of NF-κB", Nutrients, 12, pp.1238~ (2020)
- 장귀영, "열처리와 추출방법에 따른 몇 가지 약초의 항산화 활성", 한국식품영양과학회지, 41(7), pp.914~920 (2012), 한국식품영양과학회[DOI]
- Jeon YJ, "Dexamethasone inhibits IL-1β gene expression in LPS stimulated RAW 264.7 cells by blocking NF-κB", Rel and AP-1 activation, 2000, pp.48, 173~183
- 조춘희, "병풀(Centella asiatica ) 추출물을 이용한 20~50대 여성의 두피 홍반개선 효과", 아시안뷰티화장품학술지, 12(6), pp.921~927 (2014), 한국피부과학연구원
- 길영숙, "병풀 지상부 조직에서 시기별 triterpene glycoside 함량 및 항산화활성 분석", 생명과학회지, 28(8), pp.917~922 (2018), 한국생명과학회[DOI]
- 김은미, "자생 엉겅퀴의 부위별 기능성 성분 및 항산화 효과", 한국식품조리과학회지, 25(4), pp.406~414 (2009), 한국식품조리과학회
- 김옥태, "병풀 잎에서 triterpene glycosides의 시기별 함량변화", 한국약용작물학회지, 10(5), pp.375~378 (2002), 한국약용작물학회
- Kim OT, "Production of triterpene glycosides form whole plant cultures of Centella asiatica (L.) Urban", Korean J Plant Biotechnology, 29, pp.281~285 (2002)
- 권민철, "병풀의 초음파 추출 시 용매에 따른 면역활성 증진 효과", 한국약용작물학회지, 16(5), pp.294~300 (2008), 한국약용작물학회
- 이지선, "제주용암해수로 숙성된 병풀 추출물의 항염 및 보습 효과", 대한화장품학회지, 45(4), pp.363~372 (2019), 사단법인 대한화장품학회[DOI]
- 이경행, "병풀 품종별 품질 특성 및 추출 용매별 항산화 활성", 한국식품영양학회지, 34(3), pp.255~262 (2021), 한국식품영양학회[DOI]
- 이승은, "털부처꽃의 채취부위 및 채취시기에 따른 일반성분 함량 및 생리활성", 한국약용작물학회지, 18(5), pp.298~304 (2010), 한국약용작물학회
- Mohd Razali NN, "Cardiovascular protective effects of Centella asiatica and its triterpenes: A review", Planta Med, 85, pp.1203~1215 (2019)
- 목지예, "엉겅퀴(Cirsium japonicum var. ussuriense) 부위별 추출물의 항산화 및 항염증 효과", 대한본초학회지, 26(4), pp.39~47 (2011), 대한본초학회
- 오희경, "씀바귀 부위별 용매추출 방법에 따른 항산화 활성 및항염증 효과 비교", 한국응용과학기술학회지, 37(6), pp.1567~1574 (2020), 한국응용과학기술학회
- 오세원, "시설 내 토경재배에서 병풀(Centella asiatica) 두 품종의 생장특성 및 생리활성 비교", 생물환경조절학회지, 30(4), pp.351~358 (2021), (사) 한국생물환경조절학회[DOI]
- Orhan IE, "Centella asiatica (L.) urban: From traditional medicine to modern medicine with neuroprotective potential", Evid Based Complement Alternat Med, 2012, pp.946259~ (2012)
- Qiu J, "Asiaticoside attenuates lipopolyssacharideinduced acute lung injury via down-redulation of NF-κB signaling pathway", Int Immunopharmacol, 26, pp.181~187 (2015)
- 성은정, "반응표면분석법을 이용한 병풀 Triterpene 성분의 초음파 추출조건 최적화", 한국식품영양과학회지, 50(3), pp.294~300 (2021), 한국식품영양과학회
- 신현영, "충주산 병풀 용매추출물의 활성성분과 항산화 및 항염증 활성", 한국식품영양학회지, 33(6), pp.692~701 (2020), 한국식품영양학회[DOI]
- Shin Hyun Young, "Interrelationship between secondary metabolites and antioxidant capacities of Centella asiatica using bivariate and multivariate correlation analyses", Applied Biological Chemistry, 64(6), pp.1~10 (2021), 한국응용생명화학회[DOI]
- Wan JY, "Antipyretic and anti-inflammatory effects of asiaticoside in lipopolysaccharide-treated rat through up-regulation of heme oxygenase-1", Phytother Res, 27, pp.1136~1142 (2013)
- Zainol MK, "Antioxidative activity and total phenolic compounds of leaf, root and petiole of four accessions of Centella asiatica (L.) Urban", Food Chem, 81, pp.575~581 (2003)