물푸레나무 껍질의 항산화 및 항균 활성
Antioxidant and Antibacterial Activities of the bark of Fraxinus Rhynchophylla Hance
2
조회
0
다운로드
7
인용
0
참고문헌
키워드
초록
In the present study, we evaluated on the antioxidant and antibacterial activities of MeOH extract of Fraxinus rhynchophylla Hance (F. rhynchophylla ). The free radical scavenging activities of the MeOH extract and its solvent fractions of F. rhynchophylla were assessed by two different methods using 2,2-diphenyl-1- picrylhydrazyl (DPPH) and ABTS. The antioxidant activities of the MeOH extract of F. rhynchophylla were 33% and 89% at 100 μg/mL on the DPPH and ABTS radical scavenging activity, respectively. In particular, the EtOAc fraction showed more potent antioxidant activities compared with the other fractions by 81% and 93% at 100 μg/mL on the DPPH and ABTS assay. Also, the antimicrobial activities of MeOH extract and its solvent fractions of F. rhynchophylla were tested using paper disc method against three bacteria such as Staphylococcus epidermidis , Staphylococcus aureus , and Escherichia coli . The MeOH extract, n-hexane, and CHCl3 fractions exhibited antimicrobial activities against Staphylococcus epidermidis and Staphylococcus aureus . These results suggest that this plant extract could be utilized as a cosmetic ingredient possessing antibacterial and antioxidant activities.
Abstract
In the present study, we evaluated on the antioxidant and antibacterial activities of MeOH extract of Fraxinus rhynchophylla Hance (F. rhynchophylla ). The free radical scavenging activities of the MeOH extract and its solvent fractions of F. rhynchophylla were assessed by two different methods using 2,2-diphenyl-1- picrylhydrazyl (DPPH) and ABTS. The antioxidant activities of the MeOH extract of F. rhynchophylla were 33% and 89% at 100 μg/mL on the DPPH and ABTS radical scavenging activity, respectively. In particular, the EtOAc fraction showed more potent antioxidant activities compared with the other fractions by 81% and 93% at 100 μg/mL on the DPPH and ABTS assay. Also, the antimicrobial activities of MeOH extract and its solvent fractions of F. rhynchophylla were tested using paper disc method against three bacteria such as Staphylococcus epidermidis , Staphylococcus aureus , and Escherichia coli . The MeOH extract, n-hexane, and CHCl3 fractions exhibited antimicrobial activities against Staphylococcus epidermidis and Staphylococcus aureus . These results suggest that this plant extract could be utilized as a cosmetic ingredient possessing antibacterial and antioxidant activities.
참고문헌 (38)
- 김선영, "참죽나무 새순 추출물의 항균 활성과 피부 보습효과에 관한 연구", 대한화장품학회지, 36(4), pp.315~321 (2010), 대한화장품학회
- 이정희, "감잎추출물의 항산화 및 항염증 효과", 아시안뷰티화장품학술지, 12(5), pp.719~724 (2014), 한국피부과학연구원
- 이준영, "물푸레나무수피로부터 분리한 페놀성 화합물이 항산화 활성 및 뇌신경세포 보호효과", 석사, pp.2013
- 이지영, "삼채 뿌리 추출물 및 분획물의 항균 및 항산화 활성", 아시안뷰티화장품학술지, 12(4), pp.533~538 (2014), 한국피부과학연구원
- 전형주, "명월초의 항산화 효과 및 화장품과 식품의 융합소재로서 기능성 평가", 아시안뷰티화장품학술지, 12(4), pp.499~507 (2014), 한국피부과학연구원
- Blois MS, "Antioxidant determination by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Brickers DR, "Oxidative stress in the pathogenesis of skin disease", J. Inves. Dermatol, 126, pp.2565~2575 (2006)
- Burkhart CC, "Classic disease revisited: Acne, A Review of immuologic and microbiologic factor", Postgrad, Med. J, 75, pp.328~ (1999)
- Cadenas, E., "Biochemistry of oxygen toxicity", Ann. Rev. Biochem, 58, pp.79~110 (1989)
- 차배천, "토복령의 항산화 활성", 생약학회지, 36(3), pp.195~200 (2005), 한국생약학회
- Cha JY, "Antioxidative activities and contents of polyphenolic compound of Cudrania tricuspidata", J. Kor. Soc. Food Sci. Nutr, 28, pp.1310~1315 (1999)
- Claude S, "Antioxidants modulate acute solar ultraviolet radiation-induced NF-kappa-B activation in a human keratinocyte cell line", Free Radical Biol. & Med, 26, pp.174~183 (1999)
- Cogen AL, "Skin microbiota: a source of disease or defense", Br. J. Dermatol, 158, pp.442~455 (2008)
- Dai J, "Plant phenolics: Extraction, analysis, and their antioxidant and anticancer properties", Molecules, 15, pp.7313~7352 (2010)
- Davies KJA, "Protein damage and degradation by oxygen radicals", J. Biol. Chem, 262, pp.9895~9901 (1987)
- 도은수, "오배자 발효추출물의 항세균활성", 대한본초학회지, 29(4), pp.21~27 (2014), 대한본초학회
- Folin O, "On phosphotungstic-phosphomolybdic compound sas color reagents", J. Biol. Chem, 12, pp.239~243 (1912)
- Hur J., "In Dongeulbogam" (1994), Yeokangchoolpansa
- Jun S, "Antifungal and Chaetomium funicola", J. Biosci. Bioeng, 90, pp.442~ (2000)
- Kim CM, "Joongyakdaesajun", pp.348~ (1998), Jung Dam
- 김태권, "폴리페놀계 천연 항산화제의 cyclodextrin inclusion complexation을 통한 안정화와 식품 보존제로의 활용", 한국식품과학회지, 35(2), pp.266~271 (2003), 한국식품과학회
- Kostova I, "Chemical components of Fraxinus species", Fitoterapia, 78, pp.85~106 (2007)
- Layton AM, "A review on the treatment of Acne Vulgaris", Int. J. Cli. Pract, 60, pp.64~ (2006)
- 이기만, "곤충병원성진균의 총 페놀 함량 및 DPPH 라디칼 소거능", 한국응용곤충학회지, 48(3), pp.377~383 (2009), 한국응용곤충학회
- 이승욱, "울릉도산 산채류 추출물의 총 폴리페놀 함량 및 항산화 활성", 한국식품과학회지, 37(2), pp.233~240 (2005), 한국식품과학회
- Leteliera ME, "DPPH and oxygen free radicals as pro-oxidant of biomolecules", Toxicology in Vitro, 22, pp.279~286 (2008)
- Lim DK, "Antioxidative activity of ethanol extract from Korea medicinal plants", Kor. J. Food Sci. Technol, 28, pp.83~89 (1996)
- Lin YL, "(-)-Epigallo catechin-3-gallate blocks the induction of nitric oxide synthase by downregulating lipopolysaccharide-induced activity of transcription factor nuclear factor", Kappa B. Mol. phamacol, 52, pp.465~472 (1997)
- Lu Y, "Antioxidant and radical scavenging activities of polyphenols from apple pomace", Food Chem, 68, pp.81~85 (2000)
- Marples RR, "The microflora of the face and acne lesions", J. Invest. Dermatol, 62, pp.326~ (1974)
- Miller NJ, "Factors influencing the antioxidant activity determined by the ABTS.+ radical cation assay", Free Radical Res, 26, pp.195~199 (1997)
- Naqui A, "Reactive oxygen intermediate in biochemistry", Ann. Rev. Biochem, 55, pp.137~166 (1986)
- Park SN, "Skin aging and antioxidant", J. Soc. Cos. Sci Kor, 23, pp.75~132 (1997)
- 박수남, "O2으로 유도된 사람 적혈구의 광용혈에 있어서 대두의 아이소플라본인 제니스테인의 보호작용", 한국식품과학회지, 35(3), pp.510~518 (2003), 한국식품과학회
- 손희경, "구기자 추출물의 이화학적 특성", 한국식품조리과학회지, 24(6), pp.905~911 (2008), 한국식품조리과학회
- Urquiaga I, "Plant polyphenol antioxidants and oxidative stress", Biol. Res, 33, pp.55~64 (2000)
- Wang MF, "Antioxidative phenolic compounds from sage (Salivia officinalis)", J. Agric. Food Chem, 46, pp.4869~4873 (1998)
- Yaar M, "Aging versus photoaging: postulated mechanisms and effectors", J. Investing Dermatol. Symp. Proc, 3, pp.47~51 (1998)