흰민들레 에탄올 추출물 및 분획물의 항산화 및 항균활성과 Tyrosinase 저해활성
Antioxidative and Antibacterial Activity and Tyrosinase Inhibitory Activity of the Extract and Fractions from Taraxacum coreanum Nakai
2
조회
0
다운로드
31
인용
0
참고문헌
키워드
초록
In this study, we investigated antioxidative activity, antibacterial activity against pathogenic strains including methicillin resistant Staphylococcus aureus (MRSA), and tyrosinase inhibitory activity in 75% ethanol extract of Taraxacum coreanum and its fractions. The total polyphenol and flavonoid contents of the extract were 238.59㎎/g and 33.18㎎/g and the total polyphenol and flavonoid contents of the ethyl acetate fraction were 427.81㎎/g and 148.90㎎/g as the highest content of fractions. In DPPH radical scavenging ability, SC_50 values of the ethyl acetate and butanol fraction were 38.40㎍/㎖and 82.28 ㎍/㎖, respectively. In antibacterial activity by the disc diffusion assay against S. aureus, S. epidermidis and MRSA, the ethyl acetate fraction showed stronger antibacterial activity than other fractions and the extract. Especially, the ethyl acetate fraction was exhibited effective antibacterial activity against MRSA. In the cytotoxicity measurement by MTT assay, the extract and fractions were exhibited Raw 264.7 cell viabilities of 96.32~143.21% as nontoxic result in concentration of 5~100 ㎍/㎖. As a result, the ethyl acetate fraction of the 75% ethanol extract from T. coreanum could be applicable to functional materials for related fields.
Abstract
In this study, we investigated antioxidative activity, antibacterial activity against pathogenic strains including methicillin resistant Staphylococcus aureus (MRSA), and tyrosinase inhibitory activity in 75% ethanol extract of Taraxacum coreanum and its fractions. The total polyphenol and flavonoid contents of the extract were 238.59㎎/g and 33.18㎎/g and the total polyphenol and flavonoid contents of the ethyl acetate fraction were 427.81㎎/g and 148.90㎎/g as the highest content of fractions. In DPPH radical scavenging ability, SC_50 values of the ethyl acetate and butanol fraction were 38.40㎍/㎖and 82.28 ㎍/㎖, respectively. In antibacterial activity by the disc diffusion assay against S. aureus, S. epidermidis and MRSA, the ethyl acetate fraction showed stronger antibacterial activity than other fractions and the extract. Especially, the ethyl acetate fraction was exhibited effective antibacterial activity against MRSA. In the cytotoxicity measurement by MTT assay, the extract and fractions were exhibited Raw 264.7 cell viabilities of 96.32~143.21% as nontoxic result in concentration of 5~100 ㎍/㎖. As a result, the ethyl acetate fraction of the 75% ethanol extract from T. coreanum could be applicable to functional materials for related fields.
참고문헌 (32)
- Akashi T, "Biosynthesis of triterpenoids in cultured cells, and regenerated and wild plant organs of Taraxacum officinale", Phytochemistry, 36, pp.303~308 (1994)
- Blois MS, "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- 최소라, "이용부위 및 채취시기에 따른 꾸지뽕나무 메탄올 추출물의 항산화성", 한국약용작물학회지, 17(2), pp.115~120 (2009), 한국약용작물학회
- Chung HT, "Nitric oxide as a bioregulator of apoptosis", Biochemical and Biophysical Research Communications, 282, pp.1075~1079 (2001)
- Fridovich I, "Superoxide dismutase an adaption to paramagnetic gas", Journal of Biological Chemistry, 264, pp.7761~7762 (1989)
- Haenen GR, "Peroxinitrite scavenging by flavonoids", Biochemical and Biophysical Research Communications, 236, pp.591~593 (1997)
- 한소희, "민들레(Taraxacum mongolicum H.) 추출분획물이 위장보호에 미치는 효능", 한국식품과학회지, 37(1), pp.84~89 (2005), 한국식품과학회
- 허성일, "민들레 추출물의 항산화 활성 및 세포독성 효과", 생약학회지, 39(3), pp.255~259 (2008), 한국생약학회
- Jung SW, "Screening of tyrosinase inhibitor from plants", Korean Journal of Food Science and Technology, 27, pp.891~896 (1995)
- Kang MJ, "Current trends of research and biological activities of dandelion", Food Industry and Nutrition, 6, pp.60~67 (2001)
- Katrin S, "Taraxacum-A review on its phytochemical and pharmacological profile", Journal of Ethnopharmacology, 107, pp.313~323 (2006)
- 김태완, "민들레 뿌리로부터 Pancreatic lipase 저해 물질의 분리", 한국식품저장유통학회지, 18(1), pp.53~58 (2011), 한국식품저장유통학회
- 이현화, "흰민들레와 서양민들레 추출물의 세포독성 및 항산화 활성 비교", 한국약용작물학회지, 16(2), pp.79~85 (2008), 한국약용작물학회
- 이경인, "MRSA에 대한 울금 추출 및 분획물의 항균활성과 항생제 증강 효과", 생약학회지, 41(1), pp.38~42 (2010), 한국생약학회
- 이경인, "흰민들레 열수 추출물 및 분획물의 피부 관련 생리활성", 생약학회지, 42(2), pp.195~200 (2011), 한국생약학회
- 이경인, "산벚나무 수피 추출물 및 용매 분획물의 여드름 원인균에 대한 항균활성과 Tyrosinase 저해 활성", 생약학회지, 42(2), pp.155~160 (2011), 한국생약학회
- 이미숙, "새로운 항생제의 개발", 대한내과학회지, 77(1), pp.35~51 (2009), 대한내과학회
- JIN LING, "초고압 및 초음파 추출공정을 이용한 매자나무 수피의 향장활성 증진", 한국약용작물학회지, 19(1), pp.54~65 (2011), 한국약용작물학회
- Liu RH, "Potential synergy of phytochemicals in cancer prevention : mechanism of action", The Journal of Nutrition, 134, pp.3479S~3485S (2004)
- Manach C, "Bioavailability and bioefficacy of polyphenols in humans", The American Journal of Clinical Nutrition, 81, pp.230S~242S (2005)
- Marcocci L, "The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761", Biochemical and Biophysical Research Communications, 201, pp.748~755 (1994)
- Marklund S, "Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase", European Journal of Biochemistry, 47, pp.468~474 (1975)
- Moreno MIN, "Comparison of the free radical-scavenging activity of propolis from several region of Argentina", Journal of Ethnopharmacology, 71, pp.109~114 (2000)
- 오영지, "천궁(Cnidium officinale Makino) 지상부 추출물의 항산화 활성 평가", 한국약용작물학회지, 18(6), pp.373~378 (2010), 한국약용작물학회
- Radi R, "Peroxynitrite oxidation of sulfhydryls, the cytotoxic potential of superoxide and nitric oxide", Journal of Biological Chemistry, 266, pp.4244~4250 (1991)
- 유민정, "6종의 약용식물 추출물의 비듬균(Malassezia furfur)에 대한 증식억제 및 항산화 효과", 한국미용학회지, 16(1), pp.120~128 (2010), 한국미용학회
- 서용창, "고추냉이 수용성 추출물의 나노 입자화를 통한 식중독 미생물에 대한 항균 활성 증진", 한국약용작물학회지, 18(6), pp.389~397 (2010), 한국약용작물학회
- 신경민, "Amygdalin의 Murine Macrophage Raw 264.7 세포에서 in vitro 항염효과", 생약학회지, 34(3), pp.223~227 (2003), 한국생약학회
- 송재훈, "항생제 내성의 국내 현황 및 대책", 대한내과학회지, 77(2), pp.143~151 (2009), 대한내과학회
- Videla LA, "Biochemical aspects of cellular oxidative stress", Archivos De Biología y Medicina Experimentales, 21, pp.85~92 (1988)
- 허명록, "토후박 추출물의 항산화 및 항당뇨 활성", 한국약용작물학회지, 18(1), pp.34~39 (2010), 한국약용작물학회
- 윤택준, "민들레 뿌리 물 추출물의 마우스 선천 및 획득 면역계에 미치는 효과", 한국식품영양학회지, 21(3), pp.275~282 (2008), 한국식품영양학회