Development of a sequence-characterized amplified region (SCAR) marker for female off-season flowering detection in date palm (Phoenix dactylifera L.)
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Date palm (Phoenix dactylifera L.: Arecaceae) is a dioecious species where only female trees bear fruits. In their natural state, date palms produce dates once a year. However, in Thailand, some trees were observed to produce dates during the off-season, despite no variations in morphology. The availability of such off-season fruits can significantly increase their market value. Interestingly, most female off-season date palms investigated in this study were obtained through micropropagation. Hence, there is an urgent need for genetic markers to distinguish female off- season flowering plantlets within tissue culture systems. In this study, we aimed to develop random amplification of polymorphic DNA-sequence characterized amplified region (RAPD-SCAR) markers for the identification of female off-season flowering date palms cultivated in Thailand. A total of 160 random decamer primers were employed to screen for specific RAPD markers in off-season flowering male and female populations. Out of these, only one primer, OPN-02, generated distinct genomic DNA patterns in female off-season flowering (FOFdp) individuals compared to female seasonal flowering genotypes. Based on the RAPD-specific sequence, specific SCAR primers denoted as FOFdpF and FOFdpR were developed. These SCAR primers amplified a single 517-bp DNA fragment, pre- dominantly found in off-season flowering populations, with an accuracy rate of 60%. These findings underscore the potential of SCAR marker technology for tracking off- season flowering in date palms. Notably, a BLAST analysis revealed a substantial similarity between the SCAR marker sequence and the transcript variant mRNA from Phoenix dactylifera encoding the SET DOMAIN GROUP 40 protein. In Arabidopsis, this protein is involved in the epigenetic regulation of flowering time. The genetic potential of the off-season flowering traits warrants further elucidation.
Abstract
Date palm (Phoenix dactylifera L.: Arecaceae) is a dioecious species where only female trees bear fruits. In their natural state, date palms produce dates once a year. However, in Thailand, some trees were observed to produce dates during the off-season, despite no variations in morphology. The availability of such off-season fruits can significantly increase their market value. Interestingly, most female off-season date palms investigated in this study were obtained through micropropagation. Hence, there is an urgent need for genetic markers to distinguish female off- season flowering plantlets within tissue culture systems. In this study, we aimed to develop random amplification of polymorphic DNA-sequence characterized amplified region (RAPD-SCAR) markers for the identification of female off-season flowering date palms cultivated in Thailand. A total of 160 random decamer primers were employed to screen for specific RAPD markers in off-season flowering male and female populations. Out of these, only one primer, OPN-02, generated distinct genomic DNA patterns in female off-season flowering (FOFdp) individuals compared to female seasonal flowering genotypes. Based on the RAPD-specific sequence, specific SCAR primers denoted as FOFdpF and FOFdpR were developed. These SCAR primers amplified a single 517-bp DNA fragment, pre- dominantly found in off-season flowering populations, with an accuracy rate of 60%. These findings underscore the potential of SCAR marker technology for tracking off- season flowering in date palms. Notably, a BLAST analysis revealed a substantial similarity between the SCAR marker sequence and the transcript variant mRNA from Phoenix dactylifera encoding the SET DOMAIN GROUP 40 protein. In Arabidopsis, this protein is involved in the epigenetic regulation of flowering time. The genetic potential of the off-season flowering traits warrants further elucidation.
참고문헌 (42)
- Abahmane L, "Recent achievements in date palm (Phoenix dactylifera L.) micropropagation from inflorescence tissues", Emir J Food Agric, 25(11), pp.863~874 (2013)[DOI]
- Abass MH, "Genotoxicity assessment of high concentrations of 2,4-D, NAA and Dicamba on date palm callus (Phoenix dactylifera L.) using protein profile and RAPD markers", J Genet Eng Biotechnol, 15, pp.287~295 (2017)[DOI]
- Abul-Soad AA, "Commercial production of tissue culture date palm (Phoenix dactylifera L.) by inflorescence technique", J Genet Eng Biotechnol, 8(2), pp.39~44 (2010)
- Al-Khalifah NS, "Early detection of genetic variation in date palms propagated from tissue culture and offshoots by DNA fingerprinting", Acta Hort, 736, pp.105~112 (2007)
- Al-Khayri JM, "Date palm micropropagation : Advances and applications", Cienc. e Agrotecnologia, 41(4), pp.347~358 (2017)[DOI]
- Al-Khayri JM, "Advances in Plant Breeding Strategies: Fruits", pp.727~771 (2018)
- Al-Qurainy F, "SCAR marker for gender identification in date palm (Phoenix dactylifera L.) at the seedling stage", Hindawi Int J Genomics, 2018, pp.1~6 (2018)[DOI]
- Al-shahib W, "The fruit of the date palm : Its possible use as the best food for the future?", Int J Food Sci Nutr, 54(4), pp.247~259 (2003)[DOI]
- Amiteye S, "Basic concepts and methodologies of DNA marker systems in plant molecular breeding", Heliyon, 7, pp.e08093~ (2021)[DOI]
- Aslam J, "Evaluation of genetic stability in somatic embryo derived plantlets of six date palm (Phoenix dactylifera L.) cultivars through RAPD based molecular marker", Sci Technol Dev, 34(1), pp.1~8 (2015)[DOI]
- Astiari NKA, "Effort to increase off-season production and fruit quality of Siam orange (Citrus nobilis var. microcarva L.) through application of mycorrhizal inoculants and auxin" (2021), IOP Publishing
- Awan FS, "Gender identification in date palm using molecular markers", Date Palm Biotechnology Protocols, 2, pp.209~225 (2017)[DOI]
- Babu KN, "Molecular Plant Taxonomy Methods and Protocols", pp.219~247 (2021)
- Bairu MW, "Somaclonal variation in plants : causes and detection methods", Plant Growth Regul, 63, pp.147~173 (2011)[DOI]
- Biswas MK, "Development and evaluation of in vitro somaclonal variation in strawberry for improved horticultural traits", Sci Hortic, 122, pp.409~416 (2009)[DOI]
- Chao CT, "The date palm (Phoenix dactylifera L.): Overview of biology, uses, and cultivation", HortScience, 42(5), pp.1077~1082 (2007)
- Cutler RW, "Development of Sequence-characterized DNA markers linked to temperature insensitivity for fruit production in longan (Dimocarpus longan Lour.) cultivars", J Agronomy & Crop Science, 193, pp.74~78 (2007)[DOI]
- Dhawan C, "Development of male-specific SCAR marker in date palm (Phoenix dactylifera L.)", Tree Genet. Genomes, 9, pp.1143~1150 (2013)[DOI]
- Dillon SC, "The SET-domain protein superfamily : protein lysine methyltransferases", Genome Biol, 6, pp.1~10 (2005)[DOI]
- El Hadrami A, "Socioeconomic and traditional importance of date palm", Emir J Food Agric, 24(5), pp.371~385 (2012)
- El Hadrami A, "Date Palm Biotechnology", pp.183~203 (2011)
- Ferreira MDS, "The role of somaclonal variation in plant genetic improvement : A systematic review", Agronomy, 13(3), pp.730~ (2023)[DOI]
- Ghnimi S, "Date fruit (Phoenix dactylifera L.): An underutilized food seeking industrial valorization", NFS J, 6, pp.1~10 (2017)[DOI]
- Husen S, "Modified off-season technology to the flowering time and fruit yield of Arumanis mango(Mangifera indica L. )", E3S Web Conf, 226, pp.1~6 (2021)
- Jain SM, "Recent advances in date palm tissue culture and mutagenesis", Acta Hort, 736, pp.205~211 (2007)
- Kamal-Eldin A, "Classification of date fruit (Phoenix dactylifera, L.) based on chemometric analysis with multivariate approach", J Food Meas. Charact, 12(2), pp.1020~1027 (2018)[DOI]
- Research and development of Date palm, "Kanoeithip C", Department of Agriculture, 1~26 (2015)
- Kulkarni VJ, "Graft-induced off-season flowering and fruiting in the mango (Mangifera indica L.)", J Hortic Sci, 61(1), pp.141~145 (1986)[DOI]
- Kumar NS, "Random amplified polymorphic DNA(RAPD)markers and its applications", Sci Vis, 11(3), pp.116~124 (2011)
- Manochai P, "Year around off season flower induction in longan(Dimocarpus longan, Lour. )trees by KClO3 applications : Potentials and problems", Sci Hortic, 104(4), pp.379~390 (2005)[DOI]
- Mazri MA, "Micropropagation of date palm : A review", Cell Dev Biol, 4(3), pp.1~5 (2015)[DOI]
- Nartvaranant P, "Practical aspect in producing off-season mango in Thailand", Acta Hort, 509, pp.661~668 (2000)
- Nasim Z, "Nonsense-mediated mRNA decay modulates Arabidopsis flowering time via the SET DOMAIN GROUP 40-FLOWERING LOCUS C module", J Exp Bot, 72(20), pp.7049~7066 (2021)[DOI]
- Nguyen QT, "Modified compact fluorescent lamps improve light-induced off-season floral stimulation in dragon fruit farming", Food Sci Nutr, 9, pp.2390~2401 (2020)[DOI]
- Premkrishnan BV, "In silico RAPD priming sites in expressed sequences and iSCAR markers for oil palm", Int J Genomics, 2012, pp.1~5 (2012)[DOI]
- Subhadrabandhu S, "Regulation off-season flowering of longan in Thailand", Acta Hort, 558, pp.193~198 (2001)
- Thorstensen T, "SET domain proteins in plant development", Biochim Biophys Acta Bioenerg, 1809, pp.407~420 (2011)[DOI]
- Torres MF, "Genus-wide sequencing supports a two-locus model for sex-determination in Phoenix", Nat Commun, 9, pp.3969~ (2018)[DOI]
- Vayalil PK, "Date fruits(Phoenix dactylifera Linn) : An emerging medicinal food", Crit Rev Food Sci Nutr, 52(3), pp.249~271 (2012)[DOI]
- Yang L, "Development of RAPD-SCAR markers for Lonicera japonica(Caprifoliaceae)variety authentication by improved RAPD and DNA cloning", Rev Biol Trop, 62(4), pp.1649~1657 (2014)
- Younis RAA, "Identification of Sex-specific DNA markers for date palm (Phoenix dactylifera L.) using RAPD and ISSR techniques", Res J Agric & Biol Sci, 4(4), pp.278~284 (2008)
- Zhang L, "Effects of two signals on flowering and FT gene expression in off-season longan", Agric Biotechnol, 5(1), pp.15~18 (2016)
