Development of a SCAR Marker for Sex Identification in Asparagus
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초록
A sex-linked random amplified polymorphic DNA (RAPD) marker was identified from Asparagus officinalis L. and was converted into a sequence-characterized amplified regions (SCAR) marker for the large-scale screening of male and female plants. A total of 100 arbitrary decamer oligonucleotide primers were used for the RAPD analysis. Among them, the primer UBC347 amplified one female-specific 400 base pair DNA. Subsequently, the amplified RAPD fragment was cloned and sequenced. The fragment was abundant in AT and shared sequence homology with retrotransposon elements. On the basis of the sequence obtained, a pair of SCAR primer was designed. The amplification product, named F400, was the same size as the respective RAPD fragment from which it was derived. The F400 SCAR marker resulted to be female-specific in the three asparagus varieties tested in this study. This SCAR marker can be used for an early and rapid identification of female and male plants during breeding programs of asparagus.
Abstract
A sex-linked random amplified polymorphic DNA (RAPD) marker was identified from Asparagus officinalis L. and was converted into a sequence-characterized amplified regions (SCAR) marker for the large-scale screening of male and female plants. A total of 100 arbitrary decamer oligonucleotide primers were used for the RAPD analysis. Among them, the primer UBC347 amplified one female-specific 400 base pair DNA. Subsequently, the amplified RAPD fragment was cloned and sequenced. The fragment was abundant in AT and shared sequence homology with retrotransposon elements. On the basis of the sequence obtained, a pair of SCAR primer was designed. The amplification product, named F400, was the same size as the respective RAPD fragment from which it was derived. The F400 SCAR marker resulted to be female-specific in the three asparagus varieties tested in this study. This SCAR marker can be used for an early and rapid identification of female and male plants during breeding programs of asparagus.
참고문헌 (34)
- Abe, T., "Promotion of flower formation by atrazine and diuron in seedling of Asparagus", Planta, 169, pp.289~291 (1986)
- Barzen, E., "Development of coupling repulsion-phase SCAR markers diagnostic for the sugar beet Rr1 allele conferring resistance to rhizomania", Mol. Breed, 3, pp.231~238 (1997)
- Dellaporta, S.L., "A plant DNA minipreparation: version Ⅱ", Plant Mol. Rep, 1, pp.19~21 (1983)
- Del Rio, A.F., "RAPD markers efficiently distinguish heterogenous populations of wild potato (Solanum)", Gen. Res. Crop Evol, 47, pp.115~121 (2000)
- Ellison, J.H., "Breeding Vegetable Crops", pp.523~569 (1986), Avi Publishing Co
- Fraser, J.A., "Chromosomal sex-determining region in animals, plants and fungi", Current. Opi. Genet. Dev, 15, pp.645~651 (2005)
- Gao, W.J., "Identification of two markers linked to the sex locus in dioecious Asparagus officinalis plants", Russian J. Plant Physiol, 54, pp.816~821 (2007)
- Gebler, P., "Identification of molecular markers for selection of supermale (YY) asparagus plants", J. Appl. Genet, 48, pp.129~131 (2007)
- Gill, G.P., "Development of sex-linked PCR markers for gender identification in Actinidia", Theor. Appl. Genet, 97, pp.439~445 (1998)
- Jamsari, A., "BAC-derived diagnostic markers for sex determination in asparagus", Theor. Appl. Genet, 108, pp.1140~1146 (2004)
- Jamilena, M., "Plant sex chromosomes: molecular structure and function", Cytogenet. Genome Res, 120, pp.255~264 (2008)
- Jiang, C., "RAPD and SCAR markers linked to the sex expression locus M in asparagus", Euphytica, 94, pp.329~333 (1997)
- Kim, C.S., "SCARs markers derived from RAPD for cultivar identification on Pyrus pyrifolia", J. Kor. Soc. Hort. Sci, 41, pp.125~128 (2000)
- 김성철, "Characterization of a RAPD Fragment Unique to Species with Hairy Fruit Skin in the Genus Actinidia", Journal of Plant Biology(한국식물학회지), 47(3), pp.210~215 (2004), 한국식물학회
- Kubituki, K., "Families and Genera of Vascular Plants", pp.125~128 (1998), Springer-Verlag Berlin
- Lin, J.J., "AFLP, A novel PCR-based assay for plant and bacterial DNA fingerprinting", Focus, 17, pp.70~71 (1995)
- Lízal, P., "Localization of seven new late-flowering mutations on the genetic map of Arabidopsis thaliana using a newly generated CAPS marker", Plant Sci, 167, pp.143~149 (2004)
- Löptein, H., "Identification of sex chromosome pair in asparagus (Asparags officinalis L.)", Z. Pflanzenzuecht, 82, pp.162~173 (1979)
- Lu, L.D., "Cloning and analyzing of the female-specific marker in the dioecious species Asparagus officinalis L", Fen Zi Xi Bao Sheng Wu Xue Bao, 39, pp.281~284 (2006)
- Michelmore, R.W., "Identification of markers linked to disease resistant genes by bulked segregant analysis: A rapid method to detect markers in specific genome regions using segregating populations", Proc. Natl. Acad. Sci. USA, 88, pp.9828~9832 (1991)
- Negi, M.S., "Identification of AFLP fragments linked to seed coat colour in Brassica juncea and conversion to SCAR marker for rapid selection", Theor. Appl. Genet, 101, pp.146~152 (2000)
- Paran, I., "Development of reliable PCR-based markers linked to downy mildew resistance in lettuce", Theor. Appl. Genet, 85, pp.985~993 (1993)
- Powell, W., "Polymorphism revealed by simple sequence repeats", Trends Plant. Sci, 1, pp.215~222 (1996)
- Reuther, C., "Handbook of Plant Cell", pp.211~239 (1984), MacMillan Publishing Co
- Reamon-Büttner, S.M., "AFLP-derived STS marker for the identification of sex in Asparags officinalis L", Theor. Appl. Genet, 100, pp.432~438 (2000)
- Soltis, D.E., "Phylogenetic relationships in Saxifragaceae sensu lato: A comparison of topologegies based on 18S rDNA and rbcL sequences", Amer. J. Bot, 84, pp.504~522 (1997)
- Telgmann-Rauber, A., "Genetic and physical maps around the sex-determining M-locus of the dioecious plant asparagus", Mol. Genet. Genomics, 278, pp.221~234 (2007)
- Thompson, J.D., "CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice", Nucl. Acids Res, 22, pp.4673~4680 (1994)
- Urasaki, N., "Digital transcriptome analysis of putative sex-determination genes in papaya (Carica papaya)", Plos One, 7, pp.e40904~ (2012)
- Vidal, J.R., "Design of grapevine (Vitis vinifera L.) cultivar-specific SCAR primers for PCR fingerprinting", Theor. Appl. Genet, 101, pp.1194~1201 (2000)
- Welsh, J., "Fingerprinting genomes using PCR with arbitrary primers", Nucleic Acids Res, 18, pp.7213~7218 (1990)
- Yanosaka, K., "Flower induction in seedling of Asparagus officinalis L. by N-phenylcarbamates", Z. Naturforch, 44, pp.226~232 (1989)
- Yeager, A.F., "Studies of mature asparagus plantings with special reference to sex, survival and rooting habits", Proc. Amer. Soc. Hort. Sci, 36, pp.513~514 (1938)
- Zhong, S., "Molecular mapping of the leaf rust resistance gene Rph6 in barley and its linkage relationships with Rph5 and Rph7", Phytopathology, 93, pp.604~609 (2003)