백서의 하악골 전방 재위치 시 하악과두 조직에서의 nitric oxide synthases 발현 양상
Expression of nitric oxide synthases in the mandibular condyle of anterior repositioned rat mandibles
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성장기 아동의 하악과두 조직에서의 골개조 과정은 연골세포, 조골세포, 파골세포가 연관되며 nitric oxide synthase (NOS)가 중요한 매개자의 하나로 작용할 것으로 생각되나 기능성 악정형술을 이용해 하악골을 전방유도시켰을 때 하악과두에서의 골개조와 NOS의 발현에 대해서는 아직 자세히 연구되어 있지 않다. 본 연구는 하악골을 기능적으로 전방위치시켰을 때 하악과두 조직의 조직학적 변화양상에 대해 살펴보고 NOS의 발현 정도를 조사하여 상관관계를 알아보고자 하였다. 35일령의 24마리의 Sprague-Dawley 백서를 3군으로 나누었고 각 군에 있어서 5마리는 실험군으로, 3마리는 대조군으로 나누었다. 실험군은 하악골 전방유도장치를 24시간 장착한 후 3, 14, 30일에 희생하였으며 조직절편은 시상면에 평행하게 절단하였고 NOS2와 NOS3의 조직면역염색을 통해 다음과 같은 결과를 얻었다. 대조군 간에는 조직학적인 변화와 NOS의 발현 변화가 뚜렷이 관찰되지는 않았으며 실험기간에 따른 조직학적 변화는 실험 14일군에서 가장 많은 신생골 형성과 조골세포 활성을 보였으며, 실험 30일군에서는 감소하는 양상으로 나타났다. 또한 모든 실험군에서 대조군에 비해 NOS2와 NOS3의 발현이 많이 나타났으며 실험기간에 따른 비교 시 NOS2와 NOS3의 발현은 실험 14일군에서 가장 많이 나타났으며 실험 30일군에서는 감소하는 양상으로 나타났다. 전체 실험군과 대조군에서 NOS2의 발현은 NOS3의 발현보다 많이 나타났고 NOS2는 실험군 과두의 비대연골, 연골하골에서 주로 발현되었다. NOS3는 실험군 과두의 골수와 모세혈관에서 주로 발현되었다. 따라서 하악과두 내의 NOS2와 NOS3의 발현조절이 하악과두 조직의 골개조에 관여함을 시사한다. (대치교정지 2010;40(4):239-249)
Abstract
Objective: The aim of this study was to identify the expression of nitric oxide synthases (NOS) in the mandibular condyle during mandible advancement by functional appliance and to correlate it with the histologic changes and bone remodeling. Methods: Twenty-four female, 35-day-old Sprague-Dawley rats were randomly divided into 3 experimental groups. In all experimental groups, the mandibles of the rats were kept in a continuous forward position with a fixed bite jumping appliance. The rats were sacrificed on the 3rd, 14th, and 30th days of experiment. More than 2 rats in each group were used for staining. Results: There were no remarkable histologic changes and NOS expression differences in the control group. The most prominent histologic changes occurred in the 14th day experimental group. NOS decreased in the 30th day experimental group. There was increased expression of NOS2 and NOS3 in all experimental groups, comparative to the control group. In all the experimental groups and control group, the expression of NOS2 was greater than that of NOS3. Conclusions: It is postulated that NOS2 and NOS3 in the mandibular condyle might play an important role in bone remodelling of the mandibular condyle. (Korean J Orthod 2010;40(4):239-249)
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