피질골절단술 후 교정력을 가하였을 때 골의 대사에 작용하는 MMP의 역할
The Role of MMPs on Orthodontic Force after Corticotomy in Rats
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Corticotomy based on the regional acceleratory phenomenon (RAP) is used to overcome several complications during orthodontic treatment. Matrix metalloproteinases (MMPs) are thought to play a cell proliferation, migration, angiogenesis and apotosis. This study compared change of expression of MMPs after tooth movement with corticotomy to those without corticotomy. 24 Sprague-Dawley rat were used for experiment. Their first maxillary molars in both side were move by closed coil spring with 50N with corticotomy on the right side. The control side was carried sham operation. All rats were euthanized 3, 7, 10 and 14 days after surgery. Tissue samples were stained with hematoxylin and eosin (H/E) and were carried immunohisotochemisry study for MMP-2, -9, -8 and -13. The changes were observed by light microscopy. There were similar bony resorption of experimental and control side in H/E stain. MMP-2 was expressed at 14 days in both side and the difference was not significant. However, the MMP-9 was expressed 3 and 7 days and decreased at 10 and 14 days in both side. The MMP-8 and MMP-13 were not expressed in all groups. Corticotomy did not affect the expressions of MMPs after tooth movement with or without surgery significantly.
Abstract
Corticotomy based on the regional acceleratory phenomenon (RAP) is used to overcome several complications during orthodontic treatment. Matrix metalloproteinases (MMPs) are thought to play a cell proliferation, migration, angiogenesis and apotosis. This study compared change of expression of MMPs after tooth movement with corticotomy to those without corticotomy. 24 Sprague-Dawley rat were used for experiment. Their first maxillary molars in both side were move by closed coil spring with 50N with corticotomy on the right side. The control side was carried sham operation. All rats were euthanized 3, 7, 10 and 14 days after surgery. Tissue samples were stained with hematoxylin and eosin (H/E) and were carried immunohisotochemisry study for MMP-2, -9, -8 and -13. The changes were observed by light microscopy. There were similar bony resorption of experimental and control side in H/E stain. MMP-2 was expressed at 14 days in both side and the difference was not significant. However, the MMP-9 was expressed 3 and 7 days and decreased at 10 and 14 days in both side. The MMP-8 and MMP-13 were not expressed in all groups. Corticotomy did not affect the expressions of MMPs after tooth movement with or without surgery significantly.
참고문헌 (21)
- Klingsberg J, "Comparative histology of changes in oral tissues of rat, hamster and monkey", J Dent Res, 39, pp.158~169 (1960)
- Baumhammers A, "Remodeling of alveolar bone", J Periodontol, 36, pp.439~442 (1965)
- Köle H, "Surgical operations on the alveolar ridge to correct occlusal abnormalities", Oral Surg Oral Med Oral Pathol, 12(5), pp.515~529 (1959)
- Köle H, "Surgical operations on the alveolar ridge to correct occlusal abnormalities", Oral Surg Oral Med Oral Pathol, 12(4), pp.413~420 (1959)
- Köle H, "Surgical operations on the alveolar ridge to correct occlusal abnormalities", Oral Surg Oral Med Oral Pathol, 12(3), pp.277~288 (1959)
- Frost HM, "The regional acceleratory phenomenon : a review", Henry Ford Hosp Med J, 31(1), pp.3~9 (1983)
- Iino S, "Acceleration of orthodontic tooth movement by alveolar corticotomy in the dog", Am J Orthod Dentofacial Orthop, 131(4), pp.448e1~448e8 (2007)
- Wilcko MT, "Accelerated osteogenic orthodontics technique : a 1-stage surgically facilitated rapid orthodontic technique with alveolar augmentation", J Oral Maxillofac Surg, 67(10), pp.2149~2159 (2009)
- Wilcko WM, "Rapid orthodontics with alveolar reshaping : two case reports of decrowding", Int J Periodontics Restorative Dent, 21(1), pp.9~19 (2001)
- Kwan Tat S, "IL-6, RANKL, TNF-alpha", IL-1 : interrelations in bone resorption pathophysiology, 2004, pp.15, 49~60
- Sorsa T, "Matrix metalloproteinases(MMPs)in oral diseases", Oral Dis, 10(6), pp.311~318 (2004)
- Uitto VJ, "Proteolytic host cell enzymes in gingival crevice fluid", Periodontol 2000, 31, pp.77~104 (2003)
- Rody WJ Jr., "Osteoclast recruitment to sites of compression in orthodontic tooth movement", Am J Orthod Dentofacial Orthop, 120, pp.477~489 (2001)
- Rygh P., "The biology of tooth movement", pp.9~28 (1989), CRC Press
- Domon S, "In situ hybridization for matrix metalloproteinase-1 and cathepsin K in rat root-resorbing tissue induced by tooth movement", Arch Oral Biol, 44, pp.907~915 (1999)
- Lauer-Fields JL, "Matrix metalloproteinases and collagen catabolism", Biopolymers, 66, pp.19~32 (2002)
- Vu TH, "Matrix metalloproteinases : effectors of development and normal physiology", Genes Dev, 14, pp.2123~2133 (2000)
- Sorsa T, "Matrixmetalloproteinases : contribution to pathogenesis, diagnosis and tre atment of periodontal inflammation", Ann Med, 38(5), pp.306~321 (2006)
- Romanelli R, "Activation of neutrophil collagenase in periodontitis", Infect Immun, 67(5), pp.2319~2326 (1999)
- Sorsa T, "Cellularsource, activation and inhibition of dental plaque collagenase", J Clin Periodontol, 22(9), pp.709~717 (1995)
- Xu L, "Characteristics of collagenase-2 from gingival crevicular fluid and peri-implant sulcular fluid in periodontitis and peri-implantitis patients : pilot study", Acta Odontol Scand, 66(4), pp.219~224 (2008)