흰쥐 턱밑샘에서 고실끈-혀신경 절단이 NOS 발현에 미치는 영향
Effects of Chorda-lingual Denervation on NOS Expression in the Rat Submandibular Gland
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초록
침샘에서 NO의 역할에 관하여 혈류 및 분비도관의 직경 조절 등이 제시되고 있다. 그러나 NOS가 침샘 종양 등에서 발현됨은 NO가 상기 작용 외에도 침샘의 발육과 성장에 관여함을 제시한다. 본 연구는 부교감신경의 절단이 침분비의 차단을 초래하고 이 결과 NOS의 발현과 분포가 변화된다는 가정 하 에 시행되었다. 오른쪽 고실끈-혀신경을 절제하고 절제 후 1일, 3일, 7일, 14일, 30일에 희생시킨 후, 면역조직화학 염색 및 RT-PCR을 통하여 NOS 발현을 관찰하였다. 턱밑샘 무게는 신경절제 3일 경과 후부터 유의하게 감소하였다. 반대측 대조군에서 침샘 무게의 보상성 변화와 조직학적 변화가 없었으며, 절제 후 염증반응도 관찰되지 않았으나 실질세포 핵이 hyperchromatic 변화를 보였다. NOS 아형은 혈관내피세포와 도관 및 신경에서 발현되었다. 신경절단 1일과 3일 후 면역조직화학염색에서 대조군 의 nNOS와 eNOS의 조직면역성은 실험군과 유사하였다. 그러나 iNOS는 실험 1, 3일 후 크게 발현이 증가되었으 며 이는 RT-PCR에서 확인되었다. 이상의 결과는 흰쥐에서 고실끈-혀신경 절단이 침샘의 무게 감소를 초래하고 이 과정에서 iNOS가 침샘의 실질 변화와 관련되어 있음을 제시하였다.
Abstract
Nitric oxide (NO) gas has been recognized to diffuse readily across the membrane and bind directly to molecule (s) inside target cells. In the salivary gland, eNOS and nNOS are constitutively expressed. iNOS was also reported to express in neoplastic salivary tissues. Regarding the role of NO in the salivary gland, it has been suggested that it may control blood flow to the glands and furthermore involve in growth and development of the gland. The present study hypothesized that denervation of parasympathetic secretomotor fibers may lead to salivary secretion dysfunction, changing NOS expression. The gland weight on the denervated side significantly decreased from 3 days after the denervation, comparing the control (p⁄0.01). Some atrophic and hyperchromatic changes, but no inflammatory reactions were found for the whole period of the experiment. All three kinds of NOS were mainly expressed in the ducts of the gland in both the control and experimental sides. Immunoreactivities of nNOS and eNOS were not noticeably different from those of the control. However, iNOS was also detected in ducts in the normal submandibular gland by immunohistochemical staining. The iNOS expression increased more than 2 times at denervated side of the gland than the control. These results suggest that NOS isoforms, especially iNOS following chorda-lingual denervation may lead to matrix loss or cell death in the salivary gland.
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