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Identification and Detection of Streptococcus anginosus Using Species-Specific 16S rDNA Primers

유소영|김병훈|국중기|김화숙|백동헌|황호길|민정범|조지선|신환선
대한구강생물학회 (Korean Academy Of Oral Biology)|2006.03|International Journal of Oral Biology|제31권 제1호|pp. 11-14

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초록

Abstract

This study was undertaken to develop PCR primers for the identification and detection of Streptococcus anginosus using species-specific forward and reverse primers. These primers targeted the variable regions of the 16S ribosomal RNA coding gene (rDNA). The primer specificity was tested against 12 S. anginosus strains and 6 different species (10 strains) of oral bacteria. The primer sensitivity was determined by testing serial dilutions of the purified genomic DNA of S. anginosus ATCC 33397T. The data showed that species-specific amplicons were obtained from all the S. anginosus strains tested, but not in the six other species. The PCR could detect as little as 0.4 pg of the chromosomal DNA from S. anginosus. This suggests that the PCR primers are highly sensitive and applicable to the detection and identifi-cation of S. anginosus.

참고문헌 (11)

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