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Mode of Cell Death and Molecular Change of Oral Squamous Carcinoma Cells Exposed to Metal Ions

김형근|박혜련|박창준|김인령|김형일
대한구강생물학회 (Korean Academy Of Oral Biology)|2006.03|International Journal of Oral Biology|제31권 제1호|pp. 15-20

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참고문헌

주제분류치의학

초록

Abstract

Concerns remain regarding the biocompatibility and adverse effects of dental casting alloys. The aim of this study was to understand the cytopathogenic effect of metal ions, which might be released from dental alloys, on oral squamous carcinoma (OSC) cells. The cellular morphology, viability, the type of cell death and molecular change in response to metal ion salt solutions including aluminum (Al), cobalt (Co), copper (Cu) and nickel (Ni) were examined. The TC50 values for the metal ions with the exception of Al were estimated to be between 400 and 600 M. The cells treated with the metal ions showed apoptotic change with the excep-tion of Al ions. Metal ion-induced apoptosis was further confirmed using flow cytometric analysis. This study showed that the cytotoxicity and the mode of cell death by metal ions clearly depend on the cell type, the type of metal ion and the duration of exposure. The protein level of Rb, a tumor suppressor that affects apoptosis para-doxically, was higher in the cells treated with Co, Cu and Ni. It is believed that apoptosis and cell damage in the OSC cells treated with Co, Cu or Ni can be evoked by the regulation of Rb.

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