Comparison between the Rapid ID 32 Strep test system and 16S rRNA gene sequencing for the identification of α-hemolytic streptococci
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Oral viridans streptococcus is the most common bacterial species present in human dental plaque. The Rapid ID 32 Strep (bioMerieux, Marcy-l‘Etoile, France) test system allows for the identification of most streptococci within 4 hours. The aim of this study was to evaluate the reliability of this test in identifying isolates of α-hemolytic oral streptococci in human dental plaque and compare it with that of the 16S rRNA sequencing method. A total of eighty-five α-hemolytic streptococcal isolates were identified using 16S rRNA gene sequencing and Rapid ID 32 Strep. The Rapid ID 32 Strep method correctly identified 80% of the species and 87% of the streptococcal groups but failed to accurately identify five isolates (three S. cristatus, one S. australis, and one S. tigurinus).
Abstract
Oral viridans streptococcus is the most common bacterial species present in human dental plaque. The Rapid ID 32 Strep (bioMerieux, Marcy-l‘Etoile, France) test system allows for the identification of most streptococci within 4 hours. The aim of this study was to evaluate the reliability of this test in identifying isolates of α-hemolytic oral streptococci in human dental plaque and compare it with that of the 16S rRNA sequencing method. A total of eighty-five α-hemolytic streptococcal isolates were identified using 16S rRNA gene sequencing and Rapid ID 32 Strep. The Rapid ID 32 Strep method correctly identified 80% of the species and 87% of the streptococcal groups but failed to accurately identify five isolates (three S. cristatus, one S. australis, and one S. tigurinus).
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