Betula Platyphylla var. Japonica Extract Prevent Ultraviolet C Lightinduced Cell Damage in Chinese Hamster Fibroblast (V79-4) Cells
2
조회
0
다운로드
2
인용
0
참고문헌
초록
The present study reports the protective properties of a total methanol extract of B. platyphylla var. japonica against ultraviolet (UV)-C irradiation. Pretreatment of Chinese hamster fibroblast (V79-4) cells with a total methanol extract significantly increased cell survival following 300 J/ m 2 of UV-C irradiation. The total methanol extract was further fractionated into 5 fractions: n-hexane, dichloromethane, ethylacetate, n-butanol and water fractions. Among these fractions, B. platyphylla var. japonica ethylacetate, butanol and water fractions showed significant protective effects against the cellular damage induced by UV-C irradiation. In order to elucidate the mechanism underlying this protective effect, DPPH (Editor note: abbreviations should be spelled out at first use.) radical scavenging and lipid peroxidation inhibitory activity were measured. Significant radical scavenging and lipid peroxidation inhibitory activities were observed for the ethylacetate fraction. In summary, the present data demonstrate that an extract of B. platyphylla var. japonica has a significant protective effect against UV-C irradiation. The underlying mechanism of this protective effect may involve radical scavenging and inhibition of lipid peroxidation by the B. platyphylla var. japonica extract.
Abstract
The present study reports the protective properties of a total methanol extract of B. platyphylla var. japonica against ultraviolet (UV)-C irradiation. Pretreatment of Chinese hamster fibroblast (V79-4) cells with a total methanol extract significantly increased cell survival following 300 J/ m 2 of UV-C irradiation. The total methanol extract was further fractionated into 5 fractions: n-hexane, dichloromethane, ethylacetate, n-butanol and water fractions. Among these fractions, B. platyphylla var. japonica ethylacetate, butanol and water fractions showed significant protective effects against the cellular damage induced by UV-C irradiation. In order to elucidate the mechanism underlying this protective effect, DPPH (Editor note: abbreviations should be spelled out at first use.) radical scavenging and lipid peroxidation inhibitory activity were measured. Significant radical scavenging and lipid peroxidation inhibitory activities were observed for the ethylacetate fraction. In summary, the present data demonstrate that an extract of B. platyphylla var. japonica has a significant protective effect against UV-C irradiation. The underlying mechanism of this protective effect may involve radical scavenging and inhibition of lipid peroxidation by the B. platyphylla var. japonica extract.
참고문헌 (19)
- Dinkova-Kostova AT, "Protection against UV-light-induced skin carcinogenesis in SKH-1 high-risk mice by sulforaphane-containing broccoli sprout extracts", Cancer Letters, 240, pp.243~252 (2006)
- Finkel T, "Oxidants, oxidative stress and the biology of ageing", Nature, 408, pp.239~247 (2000)
- Han DH, "Antioxidant and apoptosis-induction effect of ellagic acid", Anticancer Research, 26, pp.3601~3606 (2006)
- Hansen MB, "Re-examination and further development of a precise and rapid dye method for measuring cell growth cell growth", cell kill, 1989, pp.119, 203~210
- Heck DE, "Solar ultraviolet radiation as a trigger of cell signal transduction", Toxicology and Applied Pharmacology, 195, pp.288~297 (2004)
- Herrlich P, "Supreme EnLIGHTenment:Damage recognition and signaling in the mammalian UV response", Molecular Cell, 29, pp.279~290 (2008)
- Huh JE, "Protective effects of butanol fraction from Betula platyphyla var. japonica on cartilage alterations in a rabbit collagenaseinduced osteoarthritis", Journal of Ethnopharmacology, 16, pp.1741~1754 (2008)
- Ibuki Y, "Radiation sources providing increased UVA", UVB ratios attenuate the apoptotic effects of the UVB waveband UVA-dose- dependently in hairless mouse skin, 2007, pp.127, 2236~2244
- Ju EM, "Antioxidant and anticancer activity of extract from Betula platyphylla var", Life Sciences, 74, pp.1013~1026 (2004)
- Kim EC, "The bark of Betula platyphylla var. japonica inhibits the development of atopic dermatitis-like skin lesions in NC", Nga mice, 2008, pp.116, 270~278
- Kim JH, "Reduction of radiation-induced chromosome aberration and apoptosis by dithiothreitol", Archives of Pharmacal Research, 21, pp.683~687 (1998)
- Lee SE, "Antioxidant activity of extract from Alpinia Katsumadai seed", Phytotherapy Research, 17, pp.1041~1047 (2003)
- Marmur J, "Ultraviolet light induced linking of deoxyribonucleic acid strands and its reversal by photoreactivating enzyme", Proceedings of the National Academy of Sciences of the United States of America, 47, pp.778~787 (1961)
- Peak MJ, "DNA to protein crosslinks and backbone breaks caused by far- and near-ultraviolet and visible radiations in mammalian cells", Basic life sciences, 38, pp.193~202 (1986)
- Rossette C, "Ultraviolet light and osmotic stress:-activiation of JNK cascade through multiple growth factor and cytokine receptors", Science, 274, pp.1194~1197 (1996)
- Rosenstein BS, "Induction of DNA strand breaks in normal human fibroblasts exposed to monochromatic ultraviolet and visible wavelengths in the 240-546 nm range", Photochemistry and Photobiology, 38, pp.51~55 (1983)
- Ullrich SE, "Sunlight and skin cancer: Lessons from the immune system", Molecular Carcinogenesis, 46, pp.629~633 (2007)
- Vayalil PK, "Green tea polyphenols prevent ultraviolet light-induced oxidative damage and matrix metalloproteinases expression in mouse skin", Journal of Investigative Dermatology, 122, pp.1480~1487 (2004)
- Wang H, "The protective effect and mechanism of soybean oil and its extracts on DNA damage in human ECV304 cells exposed to UV-C", Biochimica et Biophysica Acta - Gene Structure and Expression, 1626, pp.19~24 (2003)