A new in vitro method for evaluating the antimicrobial activity of toothpaste
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The purpose of this study was to introduce a new in vitro method for evaluating the antimicrobial activity of toothpaste, reflecting the actual toothbrushing time and the dilution of toothpaste by salivation. We designed three experimental groups and one negative control group. The experimental groups were (1) 90 μL of toothpaste + 10 μL 1X phosphate-buffered saline (PBS, 9/10 dilution group), (2) 50 μL of toothpaste + 40 μL 1X PBS (1/2 dilution group), and (3) 25 μL of toothpaste + 65 μL 1X PBS (1/4 dilution group). During toothbrushing, saliva is continuously secreted into the oral cavity and the toothpaste concentration is diluted over time during toothbrushing. Therefore, the 1/2 and 1/4 dilution experimental groups were added. The negative control group was toothpaste diluted 20,000-fold with 1X PBS. Miracle Fresh Doctor toothpaste and Streptococcus mitis KCOM 1350, Prevotella intermedia KCOM 1107, Fusobacterium nucleatum subsp. polymorphum KCOM 1322, and Aggregatibacter actinomycetemcomitans KCOM 1306 were used as the toothpaste and target bacterial strains, respectively. The number of bacterial cells plated on agar plates in the negative control group was 1,000 CFU. If the number of colonies on the experimental group plate was less than one, the treatment was considered to have > 99.9% bactericidal activity. These results suggest that this new in vitro method for antimicrobial evaluation could be used as the standard method for testing the antimicrobial activity of toothpaste.
Abstract
The purpose of this study was to introduce a new in vitro method for evaluating the antimicrobial activity of toothpaste, reflecting the actual toothbrushing time and the dilution of toothpaste by salivation. We designed three experimental groups and one negative control group. The experimental groups were (1) 90 μL of toothpaste + 10 μL 1X phosphate-buffered saline (PBS, 9/10 dilution group), (2) 50 μL of toothpaste + 40 μL 1X PBS (1/2 dilution group), and (3) 25 μL of toothpaste + 65 μL 1X PBS (1/4 dilution group). During toothbrushing, saliva is continuously secreted into the oral cavity and the toothpaste concentration is diluted over time during toothbrushing. Therefore, the 1/2 and 1/4 dilution experimental groups were added. The negative control group was toothpaste diluted 20,000-fold with 1X PBS. Miracle Fresh Doctor toothpaste and Streptococcus mitis KCOM 1350, Prevotella intermedia KCOM 1107, Fusobacterium nucleatum subsp. polymorphum KCOM 1322, and Aggregatibacter actinomycetemcomitans KCOM 1306 were used as the toothpaste and target bacterial strains, respectively. The number of bacterial cells plated on agar plates in the negative control group was 1,000 CFU. If the number of colonies on the experimental group plate was less than one, the treatment was considered to have > 99.9% bactericidal activity. These results suggest that this new in vitro method for antimicrobial evaluation could be used as the standard method for testing the antimicrobial activity of toothpaste.
참고문헌 (11)
- Lippert F., "An introduction to toothpaste - its purpose, history and ingredients", Monogr Oral Sci, 23, pp.1~14 (2013)[DOI]
- Ahmed F, "Antimicrobial efficacy of nanosilver and chitosan against Streptococcus mutans, as an ingredient of toothpaste formulation:an in vitro study", J Indian Soc Pedod Prev Dent, 37, pp.46~54 (2019)[DOI]
- Jenner F, "Evaluating the antimicrobial activity of commercially available herbal toothpastes on microorganisms associated with diabetes mellitus", J Contemp Dent Pract, 14, pp.924~929 (2013)[DOI]
- Junevičius J, "Antimicrobial activity of silver and gold in toothpastes : a comparative analysis", Stomatologija, 17, pp.9~12 (2015)
- Smolarek PC, "In vitro antimicrobial evaluation of toothpastes with natural compounds", Eur J Dent, 9, pp.580~586 (2015)[DOI]
- Park SN, "Development of quantitative realtime PCR primers for detecting 42 oral bacterial species", Arch Microbiol, 195, pp.473~482 (2013)[DOI]
- Loesche WJ, "Role of Streptococcus mutans in human dental decay", Microbiol Rev, 50, pp.353~380 (1986)[DOI]
- Socransky SS, "Microbial complexes in subgingival plaque", J Clin Periodontol, 25, pp.134~144 (1998)[DOI]
- Farrell JJ, "Variations of oral microbiota are associated with pancreatic diseases including pancreatic cancer", Gut, 61, pp.582~588 (2012)[DOI]
- Pietiäinen M, "Aggregatibacter actinomycetemcomitans serotypes associate with periodontal and coronary artery disease status", J Clin Periodontol, 45, pp.413~421 (2018)[DOI]
- Schenkein HA, "Mechanisms underlying the association between periodontitis and atherosclerotic disease", Periodontol 2000, 83, pp.90~106 (2020)[DOI]