Antimicrobial Effect of Commercially Available Mouth Rinsing Solutions and Natural Herbal Extracts on Streptococcus mutans
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초록
This study attempted to identify the possibility of natural herbal extracts as an alternative, preventive agent of caries by comparing antimicrobial activities between natural herbal extracts and mouth rinsing solutions against Streptococcus mutans. Natural herbal plants were extracted with distilled water and ethanol, respectively, to measure the minimum growth inhibitory concentration of S. mutans depending on concentration, and among which, solvents showing high antimicrobial activity were selected to compare their antibiotic effects with those of mouth rinsing solutions. Also, to determine the concentration of natural medicinal herbs that can be used safely in the oral cavity, the extracts were treated to the normal gingival fibroblast cells depending on concentration in order to determine its cytotoxicity using MTT. In terms of the minimum growth inhibition concentration, the growth inhibition of S. mutans was more excellent in the ethanol extract than in the distilled water. When the minimum growth inhibition concentration was compared, Psoralea corylifolia of natural herbal ethanol extracts, and Hexamedine (Bukwang Pharm., Korea) of mouth rinsing solutions inhibited growth of S. mutans at the lowest concentration. When the minimum bactericidal concentration was compared, P. corylifolia of natural herbal extracts, and Hexamedine and Garglin (Dong-A Pharm., Korea) of mouth rinsing solutions eliminated S. mutans at a low concentration. The human gingival fibroblast was treated with natural herbal ethanol extracts at the minimum growth inhibition concentration of 10, 39, and 78 g/ml. As the result, no cytotoxicity was found. When this was treated at different minimum bactericidal concentrations, natural herbal ethanol extracts showed cytotoxicity except P. corylifolia.
Abstract
This study attempted to identify the possibility of natural herbal extracts as an alternative, preventive agent of caries by comparing antimicrobialactivities between natural herbal extracts and mouth rinsing solutions against Streptococcus mutans. Natural herbal plants were extracted withdistilled water and ethanol, respectively, to measure the minimum growth inhibitory concentration of S. mutans depending on concentration, andamong which, solvents showing high antimicrobial activity were selected to compare their antibiotic effects with those of mouth rinsing solutions. Also, to determine the concentration of natural medicinal herbs that can be used safely in the oral cavity, the extracts were treated to the normalgingival fibroblast cells depending on concentration in order to determine its cytotoxicity using MTT. In terms of the minimum growth inhibitionconcentration, the growth inhibition of S. mutans was more excellent in the ethanol extract than in the distilled water. When the minimum growthinhibition concentration was compared, Psoralea corylifolia of natural herbal ethanol extracts, and Hexamedine (Bukwang Pharm., Korea) of mouthrinsing solutions inhibited growth of S. mutans at the lowest concentration. When the minimum bactericidal concentration was compared, P. corylifolia of natural herbal extracts, and Hexamedine and Garglin (Dong-A Pharm., Korea) of mouth rinsing solutions eliminated S. mutans at alow concentration. The human gingival fibroblast was treated with natural herbal ethanol extracts at the minimum growth inhibition concentrationof 10, 39, and 78 g/ml. As the result, no cytotoxicity was found. When this was treated at different minimum bactericidal concentrations, naturalherbal ethanol extracts showed cytotoxicity except P. corylifolia.
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