Effects of antibacterial mouth rinses on multiple oral biofilms model
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objectives: To confirm the antibacterial effects of each mouth rinse on multiple oral biofilms in vitro. Methods: The antibacterial effects of different mouth rinses were examined by ATP and counted colony forming units (CFU). Preformed oral biofilms on saliva coated hydroxyapatite (sHA) disks were treated with essential oil and saline; then, the multiple oral biofilms were observed by Scanning electron microscope (SEM). RNA sequencing analysis was performed on total RNA isolated from old biofilms of P. intermedia ATCC 49046. Results: In the CFU measured result compared to controls, preformed multiple oral biofilms were reduced from a low of 39.0% to 95.7% (p <0.05). The size of bacterial cells changed after treatment with the essential oil, and some of the cells ruptured into small pieces of cell debris. Gene expression in P. intermedia ATCC 49046 significantly altered in RNA transcribed and protein translated genes after exposure to essential oil. Conclusions: Mouth rinse solutions with different ingredients had different antibacterial effects and may alter surface structure and gene expression as determined by RNA sequencing.
Abstract
Objectives: To confirm the antibacterial effects of each mouth rinse on multiple oral biofilms in vitro. Methods: The antibacterial effects of different mouth rinses were examined by ATP and counted colony forming units (CFU). Preformed oral biofilms on saliva coated hydroxyapatite (sHA) disks were treated with essential oil and saline; then, the multiple oral biofilms were observed by Scanning electron microscope (SEM). RNA sequencing analysis was performed on total RNA isolated from old biofilms of P. intermedia ATCC 49046. Results: In the CFU measured result compared to controls, preformed multiple oral biofilms were reduced from a low of 39.0% to 95.7% (p <0.05). The size of bacterial cells changed after treatment with the essential oil, and some of the cells ruptured into small pieces of cell debris. Gene expression in P. intermedia ATCC 49046 significantly altered in RNA transcribed and protein translated genes after exposure to essential oil. Conclusions: Mouth rinse solutions with different ingredients had different antibacterial effects and may alter surface structure and gene expression as determined by RNA sequencing.
참고문헌 (30)
- Bruce J. Paster, "The breadth of bacterial diversity in the human periodontal pocket and other oral sites", Periodontology 2000, 42(1), pp.80~87 (2006), Wiley[DOI]
- E.A.M. Kidd, "What Constitutes Dental Caries? Histopathology of Carious Enamel and Dentin Related to the Action of Cariogenic Biofilms", Journal of Dental Research, 83(1_suppl), pp.35~38 (2004), SAGE Publications[DOI]
- P.D. Marsh, "Microbial Ecology of Dental Plaque and its Significance in Health and Disease", Advances in Dental Research, 8(2), pp.263~271 (1994), SAGE Publications[DOI]
- Benoit S, "Role of Fusobacterium nucleatum in periodontal health and disease", Current Issues in Molecular Biology, 13(2), pp.25~36 (2011), MDPI AG[DOI]
- Akira Yano, "Inhibitory effects of the phenolic fraction from the pomace of Vitis coignetiae on biofilm formation by Streptococcus mutans", Archives of Oral Biology, 57(6), pp.711~719 (2012), Elsevier BV[DOI]
- Lei Cheng, "Antibacterial and physical properties of calcium–phosphate and calcium–fluoride nanocomposites with chlorhexidine", Dental Materials, 28(5), pp.573~583 (2012), Elsevier BV[DOI]
- Frank H. Compton, "Inhibitory effect of benzethonium and zinc chloride mouthrinses on human dental plaque and gingivitis", Journal of Clinical Periodontology, 2(1), pp.33~43 (1975), Wiley[DOI]
- Norton M. Ross, "Effect of rinsing time on antiplaque‐antigingivitis efficacy of listerine", Journal of Clinical Periodontology, 20(4), pp.279~281 (1993), Wiley[DOI]
- Pekka Laine, "One‐year trial of the effect of rinsing with an amine fluoride‐stannous‐fluoride‐containing mouthwash on gingival index scores and salivary microbial counts in lymphoma patients receiving cytostatic drugs", Journal of Clinical Periodontology, 20(9), pp.628~634 (1993), Wiley[DOI]
- JamesR Collins, "In vitro microbiological analysis on antibacterial, anti-inflammatory, and inhibitory action on matrix metalloproteinases-8 of commercially available chlorhexidine digluconate mouth rinses", Indian Journal of Dental Research, 29(6), pp.799~ (2018), Medknow[DOI]
- Hiroyuki Wakabayashi, "Inhibitory Effects of lactoferrin on growth and biofilm formation of Porphyromonas gingivalis and Prevotella intermedia", Antimicrobial Agents and Chemotherapy, 53(8), pp.3308~3316 (2009), American Society for Microbiology[DOI]
- W. B. Clark, "Comparative Estimates of Bacterial Affinities and Adsorption Sites on Hydroxyapatite Surfaces", Infection and Immunity, 19(3), pp.846~853 (1978), American Society for Microbiology[DOI]
- John Alvarez, "CRABS CLAW and SPATULA, two Arabidopsis genes that control carpel development in parallel with AGAMOUS", Development, 126(11), pp.2377~2386 (1999), The Company of Biologists[DOI]
- Robert C Gentleman, "Bioconductor: open software development for computational biology and bioinformatics", Genome Biology, 5(10), pp.R80~ (2004), Springer Science and Business Media LLC[DOI]
- Vincent Zijnge, "Oral Biofilm Architecture on Natural Teeth", PLoS ONE, 5(2), pp.e9321~ (2010), Public Library of Science (PLoS)[DOI]
- Ian Kroes, "Bacterial diversity within the human subgingival crevice", Proceedings of the National Academy of Sciences, 96(25), pp.14547~14552 (1999), Proceedings of the National Academy of Sciences[DOI]
- CHRISTINE H. CHARLES, "Comparative efficacy of an antiseptic mouthrinse and an antiplaque", antigingivitis dentifrice, 2001, pp.132(5), 670~675[DOI]
- Irwin D. Mandel, "Chemotherapeutic agents for controlling plaque and gingivitis", Journal of Clinical Periodontology, 15(8), pp.488~498 (1988), Wiley[DOI]
- Kasuga Y, "Bactericidal effects of mouth rinses on oral bacteria", Bull Tokyo Dent Coll, 38(4), pp.297~302 (1997)
- D. H. Fine, "Effect of an essential oil-containing antiseptic mouthrinse on plaque and salivary Streptococcus mutans levels", Journal of Clinical Periodontology, 27(3), pp.157~161 (2000), Wiley[DOI]
- 심재용, "Antiplaque and antigingivitis effects of a mouthrinse containing cetylpyridinium chloride, triclosan and dipotassium glycyrrhizinate", Journal of Periodontal & Implant Science, 42(2), pp.33~38 (2012), 대한치주과학회[DOI]
- Takehiro Oyanagi, "Potentials of Mouthwashes in Disinfecting Cariogenic Bacteria and Biofilms Leading to Inhibition of Caries", The Open Dentistry Journal, 6(1), pp.23~30 (2012), Bentham Science Publishers Ltd.[DOI]
- C. H. Charles, "Comparative antiplaque and antigingivitis effectiveness of a chlorhexidine and an essential oil mouthrinse: 6‐month clinical trial", Journal of Clinical Periodontology, 31(10), pp.878~884 (2004), Wiley[DOI]
- Ya Shen, "Antimicrobial Efficacy of Chlorhexidine against Bacteria in Biofilms at Different Stages of Development", Journal of Endodontics, 37(5), pp.657~661 (2011), Elsevier BV[DOI]
- Yu-Chao Chang, "The effect of sodium hypochlorite and chlorhexidine on cultured human periodontal ligament cells", Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 92(4), pp.446~450 (2001), Elsevier BV[DOI]
- M. Giannelli, "Effect of chlorhexidine digluconate on different cell types: A molecular and ultrastructural investigation", Toxicology in Vitro, 22(2), pp.308~317 (2008), Elsevier BV[DOI]
- MARZENA WYGANOWSKA-SWIATKOWSKA, "Clinical implications of the growth-suppressive effects of chlorhexidine at low and high concentrations on human gingival fibroblasts and changes in morphology", International Journal of Molecular Medicine, 37(6), pp.1594~1600 (2016), Spandidos Publications[DOI]
- Luiz Fernando Noira Passos da Costa, "Chlorhexidine mouthwash as an adjunct to mechanical therapy in chronic periodontitis", The Journal of the American Dental Association, 148(5), pp.308~318 (2017), Elsevier BV[DOI]
- M.P.C. Van Leeuwen, "Essential Oils Compared to Chlorhexidine With Respect to Plaque and Parameters of Gingival Inflammation: A Systematic Review", Journal of Periodontology, 82(2), pp.174~194 (2011), Wiley[DOI]
- Jelena Marinković, "EO-based mouthwashes: Is there something that should be known?", Balkan Journal of Dental Medicine, 27(1), pp.32~37 (2023), Centre for Evaluation in Education and Science (CEON[DOI]