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Anti-proliferative and Apoptosis Inducing Effect of Resveratrol on Human Osteogenic Sarcoma (HOS) Cells

김정희|권희영|한동훈
대한구강생물학회 (Korean Academy Of Oral Biology)|2005.12|International Journal of Oral Biology|제30권 제4호|pp. 111-116

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주제분류치의학

초록

Abstract

Resveratrol (3,4',5-trihydroxy-trans-stilbene), a naturally occuring polyphenol compound which present in the skin of grapes and red wine has been considered to posses chemopreventive and antioxidant properties. However, little is known about the cellular actions by which resveratrol mediates its therapeutic effects. In this study, the effect of resveratrol on cell proliferation and induction of apoptosis in human osteogenic sarcoma (HOS) cells was investigated. IC50 value was determined to be approximately 6.0 μg/ml. Chromosomal DNA framgmentation analysis showed the appearance degraded DNA in timeand dose-dependent manner upon treatment of resveratrol. In order to observe the molecular mechanism involved in resveratrol-induced apoptosis, Western blot analysis was performed. We observed the decrease in the level of procaspase-3, the zymogen form of active caspase-3 in resveratrol-treated cells. This result implies that caspase-3 is activated upon treatment of resveratrol. The activation of caspase-3 was confirmed by the cleavage of poly(ADPribose) polymerase. Taken together, our data demonstrate that resveratrol has anti-proliferative effect on HOS cells and induced apoptosis through activation of caspase-3 and PARP cleavage.

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