Induction of p53-dependent Apoptosis by Resveratrol in Human Cancer Cells, A549 and SKOV3
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Resveratrol, a polyphenolic compound present in many fruits and vegetables such as grapes, mulberries, and peanuts, has been reported to have various biological effects. However, the molecular mechanisms underlying resveratrol-induced apoptosis in A549 ovarian cancer cells are not well understood. In this study, we investigated the effect of resveratrol on A549 lung cancer cells (expressing wild-type p53) and compared it with that observed for SKOV3 ovarian cancer cells (expressing null-type p53). Resveratrol significantly inhibited the viability and proliferation of A549 cells in a concentration- and time-dependent manner, compared with its effects on SKOV3 cells. It also induced A549 cell apoptosis, but did not affect anoikis resistance. Furthermore, the viability and proliferation of p53-knockdown A549 cells were unaffected by the presence of resveratrol. Therefore, we demonstrate that the anticancer effect of resveratrol against A549 lung cancer cells is dependent on the presence of functional p53.
Abstract
Resveratrol, a polyphenolic compound present in many fruits and vegetables such as grapes, mulberries, and peanuts, has been reported to have various biological effects. However, the molecular mechanisms underlying resveratrol-induced apoptosis in A549 ovarian cancer cells are not well understood. In this study, we investigated the effect of resveratrol on A549 lung cancer cells (expressing wild-type p53) and compared it with that observed for SKOV3 ovarian cancer cells (expressing null-type p53). Resveratrol significantly inhibited the viability and proliferation of A549 cells in a concentration- and time-dependent manner, compared with its effects on SKOV3 cells. It also induced A549 cell apoptosis, but did not affect anoikis resistance. Furthermore, the viability and proliferation of p53-knockdown A549 cells were unaffected by the presence of resveratrol. Therefore, we demonstrate that the anticancer effect of resveratrol against A549 lung cancer cells is dependent on the presence of functional p53.
참고문헌 (18)
- Chen J, "Research on the antitumor effect of ginsenoside Rg3 in B16 melanoma cells", Melanoma Res, 18, pp.322~329 (2008)
- Corney DC, "MicroRNA-34b and MicroRNA-34c are targets of p53 and cooperate in control of cell proliferation and adhesion-independent growth", Cancer Res, 67, pp.8433~8438 (2007)
- Ferraz da Costa DC, "Transient transfection of a wild-type p53 gene triggers resveratrol-induced apoptosis in cancer cells", PLoS One, 7, pp.e48746~ (2012)
- Fiucci G, "Caveolin-1 inhibits anchorage-independent growth, anoikis and invasiveness in MCF-7 human breast cancer cells", Oncogene, 21, pp.2365~2375 (2002)
- Hsieh TC, "Resveratrol increases nitric oxide synthase, induces accumulation of p53and p21(WAF1", CIP1), and suppresses cultured bovine pulmonary artery endothelial cell proliferation by perturbing progression through S and G2, 1999, pp.59, 2596~2601
- Kang SW, "Role of reactive oxygen species in cell death pathways", Hanyang Med. Rev, 33, pp.77~82 (2013)
- 진수정, "포황 메탄올 추출물에 의한 인체 대장암 세포주 HT29의 G2", M Arrest 및 Apoptosis 유발, 2013, pp.41(4), 425~432[DOI]
- 김현희, "S-allylcysteine의 자궁경부암세포주 HeLa에 대한 세포증식 억제효과", 생명과학회지, 25(4), pp.397~405 (2015), 한국생명과학회
- 이솔화, "레스베라트롤의 HT-29 대장암 세포증식 및 이동성 억제효과", KSBB Journal, 27(5), pp.289~294 (2012), 한국생물공학회
- Min SO, "Sphere-forming cultivation of liver cancer cell line in culture media without growth factors", Korean J. Clinical Oncol, 11, pp.20~23 (2015)
- Mukhopadhyay T, "Induction of apoptosis in human lung cancer cells after wild-type p53 activation by methoxyestradiol", Oncogene, 14, pp.379~384 (1997)
- NaveenKumar SK, "Unconjugated bilirubin exerts pro-apoptotic effect on platelets via p38-MAPK activation", Sci. Rep, 5, pp.15045~ (2015)
- Opipari AW Jr, "Resveratrol-induced autophagocytosis in ovarian cancer cells", Cancer Res, 64, pp.696~703 (2004)
- 박현진, "Endlicheria anomala 메탄올 추출물에 의한 인체 폐암세포주와 간암세포주의 자가사멸 유도", 생명과학회지, 25(4), pp.441~449 (2015), 한국생명과학회
- 박소영, "목향 헥산추출물의 LNCaP 전립선암세포 증식 억제 효과", 한국식품영양과학회지, 37(1), pp.8~15 (2008), 한국식품영양과학회
- Wang H, "Resveratrol inhibits TGF-beta1-induced epithelial-to-mesenchymal transition and suppresses lung cancer invasion and metastasis", Toxicology, 303, pp.139~146 (2013)
- Wang X, "Inhibition of caspase-mediated anoikis is critical for basic fibroblast growth factor-sustained culture of human pluripotent stem cells", J. Biol. Chem, 284, pp.34054~34064 (2009)
- Yoo J, "Expression of Ecadherin and p53 proteins in human soft tissue sarcomas", Arch Pathol. Lab. Med, 126, pp.33~38 (2002)