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사람뼈 육종세포에서 eugenol에 의해 유발되는 apoptosis의 기작

The mechanism of apoptosis induced by eugenol in human osteosarcoma cells

신상훈|박재현|박봉수|김철훈|길영기|김규천
대한구강악안면외과학회 (Korean Association Of Oral And Maxillofacial Surgeons)|2007.02|대한구강악안면외과학회지 (Journal of the Korean Association of Oral and Maxillofacial Surgeons)|제33권 제1호|pp. 20-27

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초록

Abstract

Eugenol is commonly used in dentistry for the sedation of toothache, pulpitis, and dental hyperalgesia. This study was performed to investigate the apoptotic effect of eugenol to human osteosarcoma (HOS) cells and the potential use of this compound in osteosarcoma cells. Eugenol showed the apoptotic effect in HOS cells in dose- and time-dependent manner. Fragmentation and condensation of DNA were showed by TUNEL assay, Hemacolor stain and Hoechst stain. In the DNA electrophoresis analysis, cells showed DNA degradation characteristic of apoptosis with a ladder pattern of DNA fragments. Apoptosis-related factors were analyzed by western blotting. Cells treated with eugenol showed caspase-3, PARP, lamin A and DFF-45 cleavage. Eugenol treatment induced caspase-3 cleavage and activation. Cleavages of PARP, DFF-45 and lamin A were accompanied with activation of caspase triggered by eugenol in HOS cells. Though this study needs more investigations, these results suggest that eugenol induce apoptosis via caspase dependent pathway in HOS cells and eugenol may constitute a potential antitumor compound against osteosarcoma cells.

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