THE ADHESION OF ODONTOBLAST TO TYPE I COLLAGEN
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Odontoblasts are anchorage dependent cells adhering to a substrate via cell adhesive molecules. Receptor ligands such as integrins bind to these proteins and are known to function as signal transduction molecules in a series of critical recognition events of cell-substratum. The aim of this study is to examine the interaction of odontoblast (MDPC-23 cell) with type I Col and the effect of TGF-β1 and TNF-αon the expression of cell adhesion molecules. In this study, MDPC-23 cells adhered to type I Col dose-dependently. Immunofluorescence data demonstrated that integrin α1, α2 and CD44 were expressed on cell surface, and FAK and paxillin were localized in focal adhesion plaques in MDPC-23 cells adhesion to Col. Cytokine TGF-β1 increased the adhesion of MDPC-23 cells to Col and the expression level of integrin α1, α2 and chondroitin sulfate on MDPC-23 cells. RT-PCR data demonstrated that cytokine TGF-β1 increased the amount of integrin α1 mRNA in MDPC-23 cells. Therefore, MDPC-23 cells adhere to collagen type I Col and expressed a complex pattern of integrins and proteoglycans,including α1, α2, chondroitin sulfate and CD44 detected by immunoblotting and immunofluorescence assay. TGF-β1 treatment enhanced the expression of adhesion molecules such as integrin α1, α2 and chondroitin sulfate.
Abstract
Odontoblasts are anchorage dependent cells adhering to a substrate via cell adhesive molecules. Receptor ligands such as integrins bind to these proteins and are known to function as signal transduction molecules in a series of critical recognition events of cell-substratum. The aim of this study is to examine the interaction of odontoblast (MDPC-23 cell) with type I Col and the effect of TGF-β1 and TNF-αon the expression of cell adhesion molecules. In this study, MDPC-23 cells adhered to type I Col dose-dependently. Immunofluorescence data demonstrated that integrin α1, α2 and CD44 were expressed on cell surface, and FAK and paxillin were localized in focal adhesion plaques in MDPC-23 cells adhesion to Col. Cytokine TGF-β1 increased the adhesion of MDPC-23 cells to Col and the expression level of integrin α1, α2 and chondroitin sulfate on MDPC-23 cells. RT-PCR data demonstrated that cytokine TGF-β1 increased the amount of integrin α1 mRNA in MDPC-23 cells. Therefore, MDPC-23 cells adhere to collagen type I Col and expressed a complex pattern of integrins and proteoglycans,including α1, α2, chondroitin sulfate and CD44 detected by immunoblotting and immunofluorescence assay. TGF-β1 treatment enhanced the expression of adhesion molecules such as integrin α1, α2 and chondroitin sulfate.
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