The Inhibitory effect of Coptis chinensis extract on the growth and the biofilm formation of Streptococcus mutans and Streptococcus sobrinus
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Streptococcus mutans and Streptococcus sobrinus play important roles in dental caries. Coptis chinensis is a natural product with antimicrobial activity against enterobacteria; however, its effects on oral streptococci are still unknown. Therefore, the effects of C. chinensis on the growth and biofilm formation of the representative cariogenic bacteria S. mutans and S. sobrinus were investigated for the possible use of C. chinensis as an anticaries agent. The C. chinensis extract was diluted with sterile distilled water, and 0.1–2.5% of the extract was used in the experiment. The effects of the C. chinensis extract on the growth and glucan formation of S. mutans and S. sobrinus were measured by viable cell counting and spectrophotometry at 650 nm absorbance, respectively. Crystal violet staining was also carried out to confirm the C. chinensis extract’s inhibitory effect on biofilm formation. The C. chinensis extract significantly inhibited the growth of S. mutans and S. sobrinus at concentrations of ≥ 0.3% as compared with the control group. The viable cell count of colonies decreased by 1.7-fold and 1.2-fold at 2.5% and 1.25%, respectively, compared with the control group. The biofilm formation of S. mutans and S. sobrinus was inhibited by > 20-fold at C. chinensis extract concentrations of ≥ 1.25% as compared with the control group. In summary, the C. chinensis extract inhibited the growth and biofilm and glucan formation of S. mutans and S. sobrinus . Therefore, C. chinensis might be a potential candidate for controlling dental caries. literature describing the oral microbiome in the context of oral health and disease is available. Advances in DNA sequencing technologies and data analysis have drastically improved the analysis of the oral microbiome. For microbiome study, bacterial 16S ribosomal RNA gene amplification and sequencing is often employed owing to the cost-effective and fast nature of the method. In this review, practical considerations for performing a microbiome study, including experimental design, molecular analysis technology, and general data analysis, will be discussed.
Abstract
Streptococcus mutans and Streptococcus sobrinus play important roles in dental caries. Coptis chinensis is a natural product with antimicrobial activity against enterobacteria; however, its effects on oral streptococci are still unknown. Therefore, the effects of C. chinensis on the growth and biofilm formation of the representative cariogenic bacteria S. mutans and S. sobrinus were investigated for the possible use of C. chinensis as an anticaries agent. The C. chinensis extract was diluted with sterile distilled water, and 0.1–2.5% of the extract was used in the experiment. The effects of the C. chinensis extract on the growth and glucan formation of S. mutans and S. sobrinus were measured by viable cell counting and spectrophotometry at 650 nm absorbance, respectively. Crystal violet staining was also carried out to confirm the C. chinensis extract’s inhibitory effect on biofilm formation. The C. chinensis extract significantly inhibited the growth of S. mutans and S. sobrinus at concentrations of ≥ 0.3% as compared with the control group. The viable cell count of colonies decreased by 1.7-fold and 1.2-fold at 2.5% and 1.25%, respectively, compared with the control group. The biofilm formation of S. mutans and S. sobrinus was inhibited by > 20-fold at C. chinensis extract concentrations of ≥ 1.25% as compared with the control group. In summary, the C. chinensis extract inhibited the growth and biofilm and glucan formation of S. mutans and S. sobrinus . Therefore, C. chinensis might be a potential candidate for controlling dental caries. literature describing the oral microbiome in the context of oral health and disease is available. Advances in DNA sequencing technologies and data analysis have drastically improved the analysis of the oral microbiome. For microbiome study, bacterial 16S ribosomal RNA gene amplification and sequencing is often employed owing to the cost-effective and fast nature of the method. In this review, practical considerations for performing a microbiome study, including experimental design, molecular analysis technology, and general data analysis, will be discussed.
참고문헌 (38)
- "Categorical data by Korean Ministry of Health and Welfare"[URL]
- Yoo YK, "The antibacterial effects of some propolis constituents against S. mutans, Lactobacilli and Actinomyces", J Korean Acad Oral Health, 20, pp.65~74 (1996)
- S. Hamada, "Virulence Factors of Streptococcus mutans and Dental Caries Prevention", Journal of Dental Research, 63(3), pp.407~411 (1984), SAGE Publications[DOI]
- 강선영, "백부자 에탄올 추출물의 Streptococcus mutans에 대한 성장, 산생성, 부착 및 비수용성 글루칸 합성억제에 미치는 영향", 동의생리병리학회지, 29(1), pp.27~32 (2015), 한의병리학회[DOI]
- Chi-Pien Chen, "Screening of Taiwanese crude drugs for antibacterial activity against Streptococcus mutans", Journal of Ethnopharmacology, 27(3), pp.285~295 (1989), Elsevier BV[DOI]
- Senji SAKANAKA, "Antibacterial substances in japanese green tea extract against Streptococcus mutans, a cariogenic bacterium", Agricultural and Biological Chemistry, 53(9), pp.2307~2311 (1989), Oxford University Press (OUP)[DOI]
- Isao. Kubo, "Antimicrobial activity of green tea flavor components and their combination effects", Journal of Agricultural and Food Chemistry, 40(2), pp.245~248 (1992), American Chemical Society (ACS)[DOI]
- S. Otake, "Anticaries Effects of Polyphenolic Compounds from Japanese Green Tea", Caries Research, 25(6), pp.438~443 (1991), S. Karger AG[DOI]
- Steinberg D, "Antibacterial effect of propolis and honey on oral bacteria", Am J Dent, 9, pp.236~239 (1996)
- Chang KW, "Effect of dietary erythritol supplemented with chitosan, extracts of Akebia and extracts of Ishige on the growth of mutans streptococci", J Korean Acad Oral Health, 21, pp.545~552 (1997)
- Chang KW, "Antibacterial effect and adsorption inhibition of oral streptococci to saliva-coated hydroxyapatite beads with Akebia quinata extract", J Korean Acad Oral Health, 21, pp.675~684 (1997)
- Jang BS, "The effects of honokiol and magnolol on the antimicrobial, bacterial collagenase activity, cytotoxicity and cytokine production", J Periodontal Implant Sci, 23, pp.145~158 (1993)
- Bae KH, "The antibacterial activites of components isolated from the stem bark of Magnolia Obovota against a cariogenic bacterium, Streptococcus mutans OMZ 176", Symp Org Chem Abstr, 176, pp.34~ (1987)
- 신강호, "Streptococcus의 유당분해에 대한 자일리톨의 효과", 대한소아치과학회지, 31(2), pp.202~211 (2004), 대한소아치과학회
- Anton C. de Groot, "Propolis: a review of properties, applications, chemical composition, contact allergy, and other adverse effects", Dermatitis, 24(6), pp.263~282 (2013), Ovid Technologies (Wolters Kluwer Health)[DOI]
- William Schumer, "Adverse effects of xylitol in parenteral alimentation", Metabolism, 20(4), pp.345~347 (1971), Elsevier BV[DOI]
- Abshenas J, "The effects of green tea(Camellia sinensis)extract on mouse semen quality after scrotal heat stress", Vet Res Forum, 2, pp.242~247 (2011)
- Mélanie Poivre, "Biological activity and toxicity of the Chinese herb Magnolia officinalis Rehder & E. Wilson (Houpo)and its constituents", Journal of Zhejiang University-SCIENCE B, 18(3), pp.194~214 (2017), Zhejiang University Press[DOI]
- Jang GH, "Anticariogenic effects of Coptis chinensis Franch extract", Korean J Food Sci Technol, 32, pp.1396~1402 (2000)
- "National Institute of Food and Drug Safety Evaluation" (2018)[URL]
- Rekik A. Muluye, "Anti-inflammatory and Antimicrobial Effects of Heat-clearing Chinese Herbs: A Current Review", Journal of Traditional and Complementary Medicine, 4(2), pp.93~98 (2014), Elsevier BV[DOI]
- M. Čerňáková, "Antimicrobial activity of berberine—a constituent ofMahonia aquifolium", Folia Microbiologica, 47(4), pp.375~378 (2002), Springer Science and Business Media LLC[DOI]
- M.L. Freile, "Antimicrobial activity of aqueous extracts and of berberine isolated from Berberis heterophylla", Fitoterapia, 74(7-8), pp.702~705 (2003), Elsevier BV[DOI]
- Marjorie Murphy Cowan, "Plant Products as Antimicrobial Agents", Clinical Microbiology Reviews, 12(4), pp.564~582 (1999), American Society for Microbiology[DOI]
- Zhong Zhen, "Anti-diabetic effects of a Coptis chinensis containing new traditional Chinese medicine formula in type 2 diabetic rats", The American Journal of Chinese Medicine, 39(01), pp.53~63 (2012), World Scientific Pub Co Pte Lt[DOI]
- Tran Manh Hung, "Magnoflorine from Coptidis Rhizoma Protects High Density Lipoprotein during Oxidant Stress", Biological and Pharmaceutical Bulletin, 30(6), pp.1157~1160 (2007), Pharmaceutical Society of Japan[DOI]
- Ahn DK, "Iiiustrated book of Korean medicinal herbs" (1998), Kyohaksa
- Liu S, "Metabolic interaction of the active constituents of coptis chinensis in human liver microsomes", Evid Based Complement Alternat Med, 2015, pp.802903~ (2015)[DOI]
- Cailan Li, "Coptisine-induced inhibition of Helicobacter pylori: elucidation of specific mechanisms by probing urease active site and its maturation process", Journal of Enzyme Inhibition and Medicinal Chemistry, 33(1), pp.1362~1375 (2018), Informa UK Limited[DOI]
- Tan L, "Epiberberine, a natural protoberberine alkaloid, inhibits urease of Helicobacter pylori and jack bean: susceptibility and mechanism", Eur J Pharm Sci, 110, pp.77~86 (2017)[DOI]
- Jiang-Tao Zhou, "Inhibition of Helicobacter pylori and Its Associated Urease by Palmatine: Investigation on the Potential Mechanism", PLOS ONE, 12(1), pp.e0168944~ (2017), Public Library of Science (PLoS)[DOI]
- Howard K. Kuramitsu, "Interspecies Interactions within Oral Microbial Communities", Microbiology and Molecular Biology Reviews, 71(4), pp.653~670 (2007), American Society for Microbiology[DOI]
- 김희은, "치아우식증 유발 균주에 대한 패러다임의 변화: 생태학적 관점", 치위생과학회지, 14(2), pp.87~93 (2014), 한국치위생과학회
- Leslie A Pratt, "Genetic analyses of bacterial biofilm formation", Current Opinion in Microbiology, 2(6), pp.598~603 (1999), Elsevier BV[DOI]
- P. Stoodley, "Biofilms as Complex Differentiated Communities", Annual Review of Microbiology, 56(1), pp.187~209 (2002), Annual Reviews[DOI]
- Philip S Stewart, "Antibiotic resistance of bacteria in biofilms", The Lancet, 358(9276), pp.135~138 (2001), Elsevier BV[DOI]
- 김강주, "백두옹 추출물이 Streptococcus mutans의 비수용성 글루칸형성 및 부착억제에 미치는 영향", 동의생리병리학회지, 30(1), pp.27~32 (2016), 한의병리학회[DOI]
- Ramona Rozen, "The role of fructans on dental biofilm formation by Streptococcus sobrinus, Streptococcus mutans, Streptococcus gordonii and Actinomyces viscosus", FEMS Microbiology Letters, 195(2), pp.205~210 (2001), Oxford University Press (OUP)[DOI]