미생물학적 원유회수증진을 위한 미얀마 저류층에서 분리한 토착미생물에 의한 표면장력 감소특성 연구
Study on Surface Tension Decrease by Indigenous Bacteria isolated from Petroleum Reservoir of Myanmar for Microbial Enhanced Oil Recovery
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미얀마 저류층에서 분리한 토착미생물이 생성하는 생물계면활성제의 표면장력 감소 능력을 조사함으로써 미생물학적 원유회수증진 기술에 대한 적용 가능성을 살펴 보았다. 탄소원의 종류에 따른 미생물의 생장과표면장력 변화를 측정한 결과, 포도당을 탄소원으로 공급하였을 떄 Bacillus spp.인 BS1과 BS2가 높은 성장률을보였으며 표면장력을 감소시키는 것으로 관찰되었다. 고온과 pH 요인이 표면장력 변화에 미치는 영향을 평가하기 위하여 배양액에서 미생물을 제거한 후 100°C의 온도 및 다양한 pH 조건에 노출시킨 결과, BS1의 생물계면활성제가 전반적으로 높은 희석비율에도 안정적인 표면장력 변화를 보여 상대적으로 외부요인의 영향을 적게받는 것으로 나타났다. BS1과 BS2가 생성한 생물계면활성제를 추출, 정제한 결과 각각 0.51 g/L, 0.26 g/L이얻어졌고 CMC 값은 각각 2.0 g/L, 0.5 g/L로 나타났다.
Abstract
For application to MEOR (microbial enhanced oil recovery) technology, the ability of biosurfactants,which were produced by indigenous bacteria isolated from a crude oil reservoir in Myanmar, to decrease surfacetension was investigated. When various carbon sources were supplied to the bacterial isolates, Bacillus spp.,designated as BS1 and BS2, showed high biomass growth and decrease in surface tension under glucose supply. Effect of pH and temperature on variation of surface tension was examined under the conditions of 100°C andpH 2, 4, 7, and 11. As a result, the biosurfactant produced by BS1 exhibited stable variation of surface tension,showing relatively low effect under various conditions. The biosurfactants produced by each bacterial isolate wereseparated and purified, and BS1 and BS2 produced the yield of biosurfactant of 0.51 g/L and 0.26 g/L. CMCvalue of biosurfactants produced by BS1 and BS2 were 2.0 g/L and 0.5 g/L, respectively.
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