분자생물학적 방법에 의한 석유 저류층 미생물 군집 분석
Analysis of Microorganisms in Petroleum Reservoir by Molecular Biological Approach
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초록
이 연구는 미생물을 이용한 석유회수증진기술에 적용 가능한 우점종 미생물을 찾아내기 위해 수행하였다. 미생물 시료는 캐나다 오일샌드와 미얀마 육상유전 것을 사용하였으며, 미생물 군집 분석은 최신 분자생물학적 방법인 파이로시퀀싱을 사용하였다. 실험은 먼저 시료로부터 저류층 미생물의 DNA를 추출한 후, 증식과정을 거쳐 우점종을 파악하였다. 실험결과 캐나다 오일샌드 지역과 미얀마 육상유전 지역의 MEOR 적용 가능한 우점종 미생물을 확인할 수 있었다. 캐나다 오일샌드 지역은 유기산과 바이오가스 등이 산출되어 탄산을 용해시켜 오일과 암석을 쉽게 분리시킬 수 있는 Streptococcus 미생물이 발견되었고, 미얀마 유전은 이산화탄소, 수소, 메탄 등이 생성되어 오일의 점도를 낮추어 주는 Clostridium 미생물이 발견되었다. 이 연구에서 정립된 미생물 군집 분석 공정은 향후, MEOR 기술개발에 활용될 수 있다.
Abstract
The purpose of this study is to analyze the dominant microorganisms in oil reservoirs to apply for MEOR. Oilsand from Canada and mid-heavy from Myanmar were sampled for the research, and the microorganisms cluster analysis was carried out by molecular biological approach. The research started with DNA extraction and amplification from the samples, and analyzed the DNA for dominant species. Throughout the research, it was able to confirm the appropriate dominant microorganisms for MEOR in Canada and Myanmar. For oilsand in Canada, Streptococcus, microorganism which degrades hydrocarbon to separate oil and sand by using produced organic acid and bio gas, was found. In reservoir of Myanmar, and Clostridium was found that it is able to improve immobility of oil by decreasing oil viscosity from CO2, H2, CH4 produced. The processes from the research can be utilized to develop MEOR techniques in the future.
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