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일엽추 추출물이 RANKL 유도 RAW264.7 Cell의 유전자 발현에 미치는 영향

Effects of Securinega suffructicosa(SS) Extract on Osteoclastogenesis and Gene Expression in RANKL-induced RAW Cell

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Objectives: This study was performed to evaluate the effect of Securinega suffructicosa Rehder(SS) on osteoclast differentiation and gene expression. Methods: The osteoclastogenesis and gene expression were determined in RANKL-stimulated RAW 264.7. The results were summarized as followes. Results: SS decreased the number of TRAP positive cell in RANKL-stimulated RAW264.7 cell. SS decreased the expression of RANK, TNFα, and IL-6 in RANKL-stimulated RAW264.7 cell. SS decreased the expression of iNOS and COX-2 in RANKL-stimulated RAW264.7 cell. SS decreased the expression of Cathepsin K in RANKL-stimulated RAW264.7 cell. Conclusions: It is concluded that SS might decrease the bone resorption resulted from decrease of osteoclast differentiation and it’s related gene expression.

Abstract

Objectives: This study was performed to evaluate the effect of Securinega suffructicosa Rehder(SS) on osteoclast differentiation and gene expression. Methods: The osteoclastogenesis and gene expression were determined in RANKL-stimulated RAW 264.7. The results were summarized as followes. Results: SS decreased the number of TRAP positive cell in RANKL-stimulated RAW264.7 cell. SS decreased the expression of RANK, TNFα, and IL-6 in RANKL-stimulated RAW264.7 cell. SS decreased the expression of iNOS and COX-2 in RANKL-stimulated RAW264.7 cell. SS decreased the expression of Cathepsin K in RANKL-stimulated RAW264.7 cell. Conclusions: It is concluded that SS might decrease the bone resorption resulted from decrease of osteoclast differentiation and it’s related gene expression.

참고문헌 (27)

  1. E. Canalis, "Mechanisms of Glucocorticoid action in Bone : Implications to glucocorticoidinduced osteoporosis", J. Clion. Endocri. Met, 81, pp.3441~3447 (1996)
  2. Pietschmann P, "Osteoporosis : an agerelated and gender-specific disease", Gerontology, 55(1), pp.3~12 (2008)
  3. Pacifici R, "Estrogen deficiency, T cells and bone loss", Cell Immunol, 252(1), pp.68~80 (2008)
  4. Kumamoto H, "Expression of parathyroid hormone-related protein (PTHrP), osteoclast differentiation factor (ODF)", receptor activator of nuclear factor-kappaB ligand (RANKL) and osteoclastogenesis inhibitory factor (OCIF), J Oral Pathol Med, pp.2004, 33(1)[DOI]
  5. Reddy SV, "Regulatory mechanisms operative in osteoclasts", Crit Rev Eukaryot Gene Expr, 14(4), pp.255~270 (2004)
  6. Boyce BF, "Roles for NF-kappaB and c-Fos in osteoclasts", J Bone Miner Metab, 23(Suppl), pp.11~15 (2005)
  7. Murakami A, "Phenethyl isothiocyanate suppresses receptor activator of NF-kappaB ligand (RANKL)- induced osteoclastogenesis by blocking activation of ERK1", 2 and p38 MAPK in RAW- 264.7 macrophages, 2007, pp.30(1), 1~11
  8. Pang M, "AP-1 stimulates the cathepsin K promoter in RAW 264.7 cells", Gene, 403(1), pp.151~158 (2007)
  9. Lee ZH, "Signal transduction by receptor activator of nuclear factor kappa B in osteoclasts", Biochem Biophys Res Commun, 305(2), pp.211~214 (2003)
  10. Lee SC, "Chemical Study on the Phenolic Compounds from the Leaves of Securinega suffruticosa", Kor J Pharmacog, 25(2), pp.105~112 (1994)
  11. Ohsaki A, "Securinega alkaloids from the wood of Securinega suffruticosa var. amamiensis", J Nat Prod, 70(12), pp.2003~2005 (2007)
  12. Lee SC, "Chemical study on the phenolic compounds from the leaves of Securinega suffruticosa", Kor J Pharmacog, 25, pp.105~ (1994)
  13. Raj D, "Securinega suffruticosa", Fitoterapia, 79(6), pp.419~427 (2008)
  14. Beutler JA, "Securinine alkaloids : A new class of GABA receptor antagonist", Brain Res, 330, pp.135~140 (1985)
  15. 장춘희, "광대싸리잎의 Acetylcholinesterase 활성 저해성분", 생약학회지, 34(1), pp.14~17 (2003), 한국생약학회
  16. Meiyanto E, "Osteoclast differentiation factor modulates cell cycle machinery and causes a delay in s phase progression in RAW264 cells", Biochem Biophys Res Commun, 282(1), pp.278~283 (2001)
  17. John T. Swarthout, "Parathyroid hormone-dependent signaling pathways regulating genes in bone cells", Gene, 282(1), pp.1~17 (2002)
  18. Eun Sook Cho, "Rolipram, a Phosphodiesterase 4 Inhibitor, Stimulates Osteoclast Formation by Inducing TRANCE Expression in Mouse Calvarial Cells", Archives of Pharmacal Research, 27(12), pp.1258~1262 (2004), 대한약학회
  19. Khosla S, "Minireview : the OPG", RANKL, Endocrinology, pp.2001, 142(12)[DOI]
  20. Gallea S, "Activation of mitogen-activated protein kinase cascades is involved in regulation of bone morphogenetic protein- 2-induced osteoblast differentiation in pluripotent C2C12 cells", Bone, 28(5), pp.491~498 (2001)
  21. Yao Z, "Tumor necrosis factor-alpha increases circulating osteoclast precursor numbers by promoting their proliferation and differentiation in the bone marrow through up-regulation of c- Fms expression", J Biol Chem, 281(17), pp.11846~11855 (2006)
  22. Liu XH, "Interactive effect of interleukin-6 and prostaglandin E2 on osteoclastogenesis via the OPG", RANKL, Ann N Y Acad Sci, pp.2006, 1068[DOI]
  23. Han SY, "Transcriptional induction of cyclooxygenase-2 in osteoclast precursors is involved in RANKL-induced osteoclastogenesis", Blood, 106(4), pp.1240~1245 (2005)
  24. Wei X, "Fibroblasts express RANKL and support osteoclastogenesis in a COX-2-dependent manner after stimulation with titanium particles", J Bone Miner Res, 20(7), pp.1136~1148 (2005)
  25. Fukushima H, "Parathyroid-hormone-related protein induces expression of receptor activator of NF-{kappa}B ligand in human periodontal ligament cells via a cAMP", protein kinase A-independent pathway, 2005, pp.84(4), 329~334
  26. Lin TH, "PPARgamma inhibits osteogenesis via the down-regulation of the expression of COX-2 and iNOS in rats", Bone, 41(4), pp.562~574 (2007)
  27. Zheng H, "RANKL stimulates inducible nitric-oxide synthase expression and nitric oxide production in developing osteoclasts. An autocrine negative feedback mechanism triggered by RANKL-induced interferon-beta via NF-kappaB that restrains osteoclastogenesis and bone resorption", J Biol Chem, 281(23), pp.15809~15820 (2006)

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