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계혈등 에탄올 추출물의 RANKL 처리 RAW264.7 세포의 분화와 염증성 골 손실에 미치는 영향

Effect of Spatholobus Suberectus Extract (SSE) on RANKL-treated RAW264.7 and LPS-induced Bone Loss

황귀서|강기성|황종현|전찬용|이대중|박도휘
대한한방내과학회 (The Society Of Korean Oriental Internal Medicine)|2022.12|대한한방내과학회지 (The Journal of Korean Oriental Internal Medicine)|제43권 제6호|pp. 1134-1148

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참고문헌

키워드

RANKLosteoclastosteoporosisSpatholobus suberectus
주제분류한의학

초록

Purpose: We evaluated whether Spatholobus suberectus extract (SSE) can be used as a means of preventing and treating osteoporosis by measuring its effect on osteoclast differentiation, gene expression, and bone resorption. Methods: SSE was used to examine the effect on RAW 264.7 cells stimulated with RANKL to induce bone resorption. The inhibitory effect of TRAP formation and the expression of the bone resorption factors TRAP, cathepsin K, and MMP-9 during differentiation were measured. The effects on the differentiation-related factors NFATc and TRAIL and on the expression of OC-STAMP, DC-STAMP, ATP6v0d2, MITF, c-Fos, and inflammation-related factors were also evaluated. The effect on bone resorption was evaluated by culturing RANKL-treated osteoclasts on artificial bone fragments and observing the resulting resorption traces. The effect on bone damage in experimental animals was also measured. Results: SSE inhibited the differentiation of RANKL-stimulated osteoclasts into osteoclasts and suppressed the expression of cathepsin K, TRAP, MMP-9, NFATc1, TRAIL, MITF, OC-STAMP, DC-STAMP, ATP6v0d2, and c-Fos genes. Bone pore formation due to osteoclast action was also inhibited, and LPS-induced bone loss was suppressed in animal experiments. Conclusions: SSE could be useful for the prevention or treatment of osteoporosis by inhibiting osteoclast differentiation and bone resorption and suppressing bone loss induced in experimental animals. However, studies of larger populations are required.

Abstract

Purpose: We evaluated whether Spatholobus suberectus extract (SSE) can be used as a means of preventing and treating osteoporosis by measuring its effect on osteoclast differentiation, gene expression, and bone resorption. Methods: SSE was used to examine the effect on RAW 264.7 cells stimulated with RANKL to induce bone resorption. The inhibitory effect of TRAP formation and the expression of the bone resorption factors TRAP, cathepsin K, and MMP-9 during differentiation were measured. The effects on the differentiation-related factors NFATc and TRAIL and on the expression of OC-STAMP, DC-STAMP, ATP6v0d2, MITF, c-Fos, and inflammation-related factors were also evaluated. The effect on bone resorption was evaluated by culturing RANKL-treated osteoclasts on artificial bone fragments and observing the resulting resorption traces. The effect on bone damage in experimental animals was also measured. Results: SSE inhibited the differentiation of RANKL-stimulated osteoclasts into osteoclasts and suppressed the expression of cathepsin K, TRAP, MMP-9, NFATc1, TRAIL, MITF, OC-STAMP, DC-STAMP, ATP6v0d2, and c-Fos genes. Bone pore formation due to osteoclast action was also inhibited, and LPS-induced bone loss was suppressed in animal experiments. Conclusions: SSE could be useful for the prevention or treatment of osteoporosis by inhibiting osteoclast differentiation and bone resorption and suppressing bone loss induced in experimental animals. However, studies of larger populations are required.

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관련 논문AI추천

치자 추출물이 RANKL 유도 파골세포 형성 및 골 흡수에 미치는 영향최유경, 황귀서대한한방내과학회 · 대한한방내과학회지2017 · 제38권 제6호일엽추 추출물이 RANKL 유도 RAW264.7 Cell의 유전자 발현에 미치는 영향이효근, 황귀서대한예방한의학회 · 대한예방한의학회지2010 · 제14권 제3호한수대보원이 포유동물인 생쥐 모델에서 골 손실 및 RANKL 유도 파골세포 분화에 미치는 영향류홍선, 장시성, 전찬용, 황귀서대한한방내과학회 · 대한한방내과학회지2019 · 제40권 제1호威靈仙이 RANKL 처리 RAW 264.7 Cell에 미치는 영향송영훈, 유동열, 유정은, 임현정대한한방부인과학회 · 대한한방부인과학회지2010 · 제23권 제3호生脈散의 파골세포 분화 억제와 관련 유전자 발현에 미치는 영향이은경, 정명수, 주인학, 황귀서대한예방한의학회 · 대한예방한의학회지2015 · 제19권 제2호白何首烏 물 추출물의 파골세포 분화에 미치는 영향문서영, 안용환, 오재민, 이명수, 전병훈, 정종혁, 채수욱, 최민규한의병리학회 · 동의생리병리학회지2012 · 제26권 제2호

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