사물탕의 용량별 투여가 임신 랫드와 태자에 미치는 독성학적 연구
Toxicological Effect of Samultang(Herbal Medicine) Administration in the Pregnant Rats and Fetuses ―Focusing on dose-response Relationship―
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Objective: Samulatang(herbal description) is much used for women’s disease in Korean Traditional Medicine. The aim of this study is to evaluate reproductive toxic effect by Samultang in pregnant rats and fetuses, and ascertain a dose-response relationship Method: Pregnant Sprague-Dawley rats were administered with the Samultang at single, double and quadruple dose for 20 days, orally. Pregnant rats were sacrificed at 20th day of gestation, and observed internal and reproductive organs. Live fetuses of gestation were randomly selected and fixed in 95% ethanol. Fetuses were stained with alcian blue and alizarin red S. We observe maternal body weight,, index associated pregnancy, and skeletal malformations in fetus Result: Maternal body weight of Samultang treated group has increased, side effect was not found in maternal body compared to that of control group. There were no significant difference in internal and reproductive organs. Double concentration administered group had lowest value in number of implantation, live fetuses, implantation rate and delivery rate, Also double concentration administered group showed higher early and late resorption rate than the other group. But, these are not significant. In the sex ratio, number of females, bigger than number of males in all Samultang administered groups. The fetuses of dams treated with Samultang didn’t showed external and skeletal malformation. Vertebral and sternal variations were observed in single, double and quadruple concentration administered group but, compared to the control, those variations were insignificant. There were no significant changes in number of ribs, cervical, thoracic, lumber, sacral and caudal vertebrae Conclusion: Samultang is not expected to affect on pregnant rats and fetus about maternal body weight and number of live fetuses. There were no significant changes in organ weight, reproductive organs. Although skeletal variations were showed in vertebrae and sternum, treated groups were shown insignificant changes in skeletal variation
Abstract
Objective: Samulatang(herbal description) is much used for women’s disease in Korean Traditional Medicine. The aim of this study is to evaluate reproductive toxic effect by Samultang in pregnant rats and fetuses, and ascertain a dose-response relationship Method: Pregnant Sprague-Dawley rats were administered with the Samultang at single, double and quadruple dose for 20 days, orally. Pregnant rats were sacrificed at 20th day of gestation, and observed internal and reproductive organs. Live fetuses of gestation were randomly selected and fixed in 95% ethanol. Fetuses were stained with alcian blue and alizarin red S. We observe maternal body weight,, index associated pregnancy, and skeletal malformations in fetus Result: Maternal body weight of Samultang treated group has increased, side effect was not found in maternal body compared to that of control group. There were no significant difference in internal and reproductive organs. Double concentration administered group had lowest value in number of implantation, live fetuses, implantation rate and delivery rate, Also double concentration administered group showed higher early and late resorption rate than the other group. But, these are not significant. In the sex ratio, number of females, bigger than number of males in all Samultang administered groups. The fetuses of dams treated with Samultang didn’t showed external and skeletal malformation. Vertebral and sternal variations were observed in single, double and quadruple concentration administered group but, compared to the control, those variations were insignificant. There were no significant changes in number of ribs, cervical, thoracic, lumber, sacral and caudal vertebrae Conclusion: Samultang is not expected to affect on pregnant rats and fetus about maternal body weight and number of live fetuses. There were no significant changes in organ weight, reproductive organs. Although skeletal variations were showed in vertebrae and sternum, treated groups were shown insignificant changes in skeletal variation
참고문헌 (29)
- Eisenberg DM, "Trend in alternative medicine use in the united states. 1990-1997. Results of followup national survey", JAMA, 280, pp.1569~1575 (1998)
- Pittler MH, "Systematic review : hepatotoxic events associated with herbal medicinal products", Aliment Pharmacol Ther, 18, pp.451~471 (2003)
- 이정석, "한․양약 복용이 간기능에미치는 영향", 대한예방한의학회지, 8(1), pp.59~74 (2004)
- 송태복, "선천성 기형의 원인", 대한주산학회지, 4(2), pp.145~152 (1993)
- 최진주, "선천성기형 10년 6개월 연구", 대한산부인과학회지, 21(12), pp.1025~1035 (1978)
- 김종일, "선천성기형에 대한 연구", 대한산부인과학회지, 35(12), pp.1720~1729 (1992)
- 김철원, "임신중 한약을 투여한 37례의 임상보고", 대한한의학회지, 19(2), pp.75~85 (1998)
- 이인호, "한약복용이 태아에 미치는 영향에 대한 실태분석연구", 대한한의학회지, 21(1), pp.40~44 (2000)
- 허지원, "꽃마을 한방병원에서 임신중 한약을 투여받은 환자 146례에 대한 실태분석연구", 대한한방부인과학회지, 15(3), pp.129~138 (2002), 대한한방부인과학회
- 정형민, "교애궁귀탕, 보중익기탕, 궁소산, 안태음, 안태금출탕을 투약한 임신생쥐의 생식능력의 변화", 대한한의학회지, 21(3), pp.166~173 (2000)
- 신헌태, "안태음이 임신랫드와 태자에 미치는 영향", 한국환경보건학회지, 33(4), pp.306~317 (2007), 한국환경보건학회
- 김창석, "보생탕이 랫드의 모체와 태자에 미치는 영향에 대한 연구", 대한예방한의학회지, 9(2), pp.59~75 (2005)
- 이선동, "달생탕이 랫드의 모체와 태자에 미치는 영향에 대한 연구", 한국환경보건학회지, 32(4), pp.342~352 (2006), 한국환경보건학회
- Doull, J, "Toxicology ; The basic science of poisons, 6th edition" (2001)
- 김판기, "임신랫드에 투여한 Butyl Benzyl Phthalate가 차산자에 미치는 영향", 한국환경위생학회지, 26(1), pp.85~94 (2000)
- 이학식, "SPSS 12.0 매뉴얼" (2005), 법문사
- 陳師文, "太平惠民和劑局方", pp.242~ (1975), 旋風出版社
- 朱震亨, "新編丹溪心法附餘(下卷)", pp.702~703 (1993), 대성문화사
- 許俊, "東醫寶鑑", pp.235~ (1981), 대성문화사
- 龔延賢, "萬病回春", pp.189~190 (1993), 醫聖堂
- 김완희, "사물탕에 대한 고찰", 대한한의학회지, 2, pp.35~41 (1966)
- 박종운, "사물탕이 노화백서 뇌조직의 생화학적 변화에 미치는 영향", 대한한방내과학회지, 19, pp.185~201 (1998)
- 조권일, "培養心筋細胞의 酸化的 損傷에 대한 四物湯의 防禦效果", 대한한의학회지, 26(1), pp.174~186 (2005), 대한한의학회
- 염정열, "사물탕이 임신 말기 생쥐의 면역능에 미치는 영향", 생약학회지, 31(2), pp.142~148 (2000)
- 이성환, "알코올로 유발된 생쥐의 위점막손상에 대한 사물탕의 보호효과", 대한한의학회지, 25(3), pp.1~11 (2004), 대한한의학회
- 배병철, "今釋黃帝內經素問" (1999), 成輔社
- 李時珍, "國譯本草綱目", pp.141~ (1978), 杏林出版社
- 陳自明, "婦人良方大全", pp.324~325 (1991), 一中社
- 최민선, "임신 중 치료목적으로 한약을 복용한 환자에 대한 실태분석 및 치료에 대한 만족도 조사", 대한한방부인과학회지, 18(3), pp.127~138 (2005), 대한한방부인과학회