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菟絲子가 RANKL 유도 破骨細胞에 미치는 영향

The Effects of Cuscuta japonica Chois on Gene Expression in RANKL-induced RAW 264.7 Cell

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Objectives:This study was performed to evaluate the effect of CJ(Cuscuta japonica Chois) on osteoclast differentiation and gene expression. Methods:The osteoclastogenesis and gene expression were determined in RANKL(receptor activator of nuclear factor kappa B ligand)-stimulated RAW 264.7. The results were summarized as followes. Results:CJ decreased the number of TRAP positive cell in RANKL-stimulated RAW264.7 cell. CJ decreased the expression of RANK(receptor activator of nuclear factor kappa B), TNFα, and IL-6 in RANKL-stimulated RAW264.7 cell. CJ decreased the expression of iNOS and COX-2 in RANKL-stimulated RAW264.7 cell. CJ decreased the expression of Cathepsin K in RANKL-stimulated RAW264.7 cell. Conclusions:It is concluded that CJ might decrease the bone resorption resulted from decrease of osteoclast differentiation and it’s related gene expression.

Abstract

Objectives:This study was performed to evaluate the effect of CJ(Cuscuta japonica Chois) on osteoclast differentiation and gene expression. Methods:The osteoclastogenesis and gene expression were determined in RANKL(receptor activator of nuclear factor kappa B ligand)-stimulated RAW 264.7. The results were summarized as followes. Results:CJ decreased the number of TRAP positive cell in RANKL-stimulated RAW264.7 cell. CJ decreased the expression of RANK(receptor activator of nuclear factor kappa B), TNFα, and IL-6 in RANKL-stimulated RAW264.7 cell. CJ decreased the expression of iNOS and COX-2 in RANKL-stimulated RAW264.7 cell. CJ decreased the expression of Cathepsin K in RANKL-stimulated RAW264.7 cell. Conclusions:It is concluded that CJ might decrease the bone resorption resulted from decrease of osteoclast differentiation and it’s related gene expression.

참고문헌 (25)

  1. Kumamoto H, "Expression of parathyroid hormone-related protein(PTHrP), osteoclast differentiation factor(ODF)", receptor activator of nuclear factor-kappaB ligand (RANKL) and osteoclastogenesis inhibitory factor(OCIF), J Oral Pathol Med, pp.2004, 33(1)[DOI]
  2. Reddy SV, "Regulatory mechanisms operative in osteoclasts", Crit Rev Eukaryot Gene Expr, 14(4), pp.255~270 (2004)
  3. Boyce BF, "Roles for NF-kappaB and c-Fos in osteoclasts", J Bone Miner Metab, 23(Suppl), pp.11~15 (2005)
  4. Murakami A, "Phenethyl isothiocyanate suppresses receptor activator of NF-kappaB ligand (RANKL)- induced osteoclastogenesis by blocking activation of ERK1", 2 and p38 MAPK in RAW264.7 macrophages, 2007, pp.30(1), 1~11
  5. Pang M, "AP-1 stimulates the cathepsin K promoter in RAW 264.7 cells", Gene, 403(1), pp.151~158 (2007)
  6. Lee ZH, "Signal transduction by receptor activator of nuclear factor kappa B in osteoclasts", Biochem Biophys Res Commun, 305(2), pp.211~214 (2003)
  7. 全國韓醫科大學 敎授, "本草學", pp.568~569 (1994), 永林出版社
  8. Ye M, "Determination of flavonoids in Semen Cuscutae by RP-HPLC", J. Pharm. Biomed. Anal, 28(3), pp.621~628 (2002)
  9. Yen FL, "Hepatoprotective and antioxidant effects of Cuscuta chinensis against acetaminopheninduced hepatotoxicity in rats", J Ethnopharmacol, 111(1), pp.123~128 (2007)
  10. 이재복, "兎絲子藥針의 抗癌作用 및 免疫效果에 對한 實驗的 硏究", 大韓鍼灸學會誌, 18, pp.94~104 (2001)
  11. Koo BS, "Neuroprotective effect of Chuk-Me-Sun-Dan on NMDAand AMPA-evoked nitric oxide synthase activity in mouse brain", Immunopharmacol Immunotoxicol, 27(3), pp.499~514 (2005)
  12. Oh H, "Angiotensin converting enzyme inhibitors from Cuscuta japonica Choisy", J Ethnopharmacol, 83(1), pp.105~108 (2002)
  13. Han SY, "Transcriptional induction of cyclooxygenase-2 in osteoclast precursors is involved in RANKL-induced osteoclastogenesis", Blood, 106(4), pp.1240~1245 (2005)
  14. John T, "Swarthout, Richard C. D’Alonzob, Nagarajan Selvamurugan and Nicola C. Partridge. Parathyroid hormone-dependent signaling pathways regulating genes in bone cells", Gene, 282(1), pp.1~17 (2002)
  15. Eun Sook Cho, "Rolipram, a Phosphodiesterase 4 Inhibitor, Stimulates Osteoclast Formation by Inducing TRANCE Expression in Mouse Calvarial Cells", Archives of Pharmacal Research, 27(12), pp.1258~1262 (2004), 대한약학회
  16. Khosla S, "Minireview: the OPG", RANKL, Endocrinology, pp.2001, 142(12)[DOI]
  17. Gallea S, "Activation of mitogen-activated protein kinase cascades is involved in regulation of bone morphogenetic protein-2- induced osteoblast differentiation in pluripotent C2C12 cells", Bone, 28(5), pp.491~498 (2001)
  18. Yao Z, "Tumor necrosis factor-alpha increases circulating osteoclast precursor numbers by promoting their proliferation and differentiation in the bone marrow through up-regulation of c-Fos expression", J Biol Chem, 281(17), pp.11846~11855 (2006)
  19. Liu XH, "Interactive effect of interleukin-6 and prostaglandin E2 on osteoclastogenesis via the OPG", RANKL, Ann N Y Acad Sci, pp.2006, 1068[DOI]
  20. Han SY, "Transcriptional induction of cyclooxygenase-2 in osteoclast precursors is involved in RANKL-induced osteoclastogenesis", Blood, 106(4), pp.1240~1245 (2005)
  21. Wei X, "Fibroblasts express RANKL and support osteoclastogenesis in a COX-2-dependent manner after stimulation with titanium particles", J Bone Miner Res, 20(7), pp.1136~1148 (2005)
  22. Fukushima H, "Parathyroid-hormone-related protein induces expression of receptor activator of NF-{kappa}B ligand in human periodontal ligament cells via a cAMP", protein kinase A-independent pathway, 2005, pp.84(4), 329~334
  23. Lin TH, "PPARgamma inhibits osteogenesis via the down-regulation of the expreCJion of COX-2 and iNOS in rats", Bone, 41(4), pp.562~574 (2007)
  24. Zheng H, "RANKL stimulates inducible nitric-oxide synthase expression and nitric oxide production in developing osteoclasts. An autocrine negative feedback mechanism triggered by RANKL-induced interferonbeta via NF-kappaB that restrains osteoclastogenesis and bone resorption", J Biol Chem, 281(23), pp.15809~15820 (2006)
  25. Kim MS, "MCP-1-induced human osteoclast-like cells are tartrateresistant acid phosphatase, NFATc1, and calcitonin receptor-positive but require receptor activator of NF-kappaB ligand for bone resorption", J Biol Chem, 281(2), pp.1274~1285 (2006)

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