Expression pattern of prohibitin, capping actin protein of muscle Z-line beta subunit and tektin-2 gene in Murrah buffalo sperm and its relationship with sperm motility
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Objective: The aim of the current study is to investigate the relationship between prohibitin (PHB), capping actin protein of muscle Z-line beta subunit (CAPZB), and tektin-2 (TEKT2) and sperm motility in Murrah buffalo. Methods: We collected the high-motility and low-motility semen samples, testis, ovary, muscle, kidney, liver, brain and pituitary from Murrah buffalo, and analysed the expression of PHB, CAPZB, and TEKT2 in mRNA (message RNA) and protein level. Results: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) result showed that the expression of PHB was higher and CAPZB, TEKT2 were specifically expressed in testis as compared to the other 6 tissues, and that in testis, the expression of TEKT2 was higher than that of CAPZB and PHB. Immunohistochemistry test revealed that all three genes were located on the convoluted seminiferous tubule and enriched in spermatogenic cells. Both qRT-PCR and Western Blot results showed that the expression levels of PHB, CAPZB, and TEKT2 were significantly lower in the low-motility semen group compared to the high-motility semen group (p<0.05). Conclusion: The expression levels of PHB, CAPZB, and TEKT2 in Murrah buffalo sperm have a high positive correlation with sperm motility. And the three genes may be potential molecular markers for the decline of buffalo sperm motility.
Abstract
Objective: The aim of the current study is to investigate the relationship between prohibitin (PHB), capping actin protein of muscle Z-line beta subunit (CAPZB), and tektin-2 (TEKT2) and sperm motility in Murrah buffalo. Methods: We collected the high-motility and low-motility semen samples, testis, ovary, muscle, kidney, liver, brain and pituitary from Murrah buffalo, and analysed the expression of PHB, CAPZB, and TEKT2 in mRNA (message RNA) and protein level. Results: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) result showed that the expression of PHB was higher and CAPZB, TEKT2 were specifically expressed in testis as compared to the other 6 tissues, and that in testis, the expression of TEKT2 was higher than that of CAPZB and PHB. Immunohistochemistry test revealed that all three genes were located on the convoluted seminiferous tubule and enriched in spermatogenic cells. Both qRT-PCR and Western Blot results showed that the expression levels of PHB, CAPZB, and TEKT2 were significantly lower in the low-motility semen group compared to the high-motility semen group (p<0.05). Conclusion: The expression levels of PHB, CAPZB, and TEKT2 in Murrah buffalo sperm have a high positive correlation with sperm motility. And the three genes may be potential molecular markers for the decline of buffalo sperm motility.
참고문헌 (42)
- Park YJ, "Proteomic revolution to improve tools for evaluating male fertility in animals", J Proteome Res, 12, pp.4738~4747 (2013)
- Rahman MS, "Sperm proteomics: road to male fertility and contraception", Int J Endocrinol, 2013 (2013)
- Dungdung SR, "Insights from animal reproduction" (2016), InTech
- Elfateh F, "Influence of genetic abnormalities on semen quality and male fertility: A four-year prospective study", Iran J Reprod Med, 12, pp.95~102 (2014)
- Tanaka H, "Mice deficient in the axonemal protein Tektin-t exhibit male infertility and immotile-cilium syndrome due to impaired inner arm dynein function", Mol Cell Biol, 24, pp.7958~7964 (2004)
- Zuccarello D, "Mutations in dynein genes in patients affected by isolated non-syndromic asthenozoospermia", Hum Reprod, 23, pp.1957~1962 (2008)
- Huang SY, "Effects of single nucleotide polymorphisms in the 5’-flanking region of heat shock protein 70.2 gene on semen quality in boars", Anim Reprod Sci, 70, pp.99~109 (2002)
- Ambruso DR, "Peroxiredoxin 6 translocates to the plasma membrane during neutrophil activation and is required for optimal NADPH oxidase activity", Biochim Biophys Acta, 1823, pp.306~315 (2012)
- Steger K, "Both protamine-1 to protamine-2 mRNA ratio and Bcl2 mRNA content in testicular spermatids and ejaculated spermatozoa discriminate between fertile and infertile men", Hum Reprod, 23, pp.11~16 (2008)
- Williams D, "Mapping of R-SNARE function at distinct intracellular GLUT4 trafficking steps in adipocytes", J Cell Biol, 180, pp.375~387 (2008)
- Amaral A, "The combined human sperm proteome: cellular pathways and implications for basic and clinical science", Hum Reprod Update, 20, pp.40~62 (2014)
- Baccetti B, "Gene deletions in an infertile man with sperm fibrous sheath dysplasia", Hum Reprod, 20, pp.2790~2794 (2005)
- Yu Y, "Molecular characterization of the sheep CIB1 gene", Mol Biol Rep, 36, pp.1799~1809 (2009)
- Mishra S, "The prohibitins: emerging roles in diverse functions", J Cell Mol Med, 10, pp.353~363 (2006)
- Merkwirth C, "Prohibitin function within mitochondria: essential roles for cell proliferation and cristae morphogenesis", Biochim Biophys Acta, 1793, pp.27~32 (2009)
- Cooper JA, "New insights into mechanism and regulation of actin capping protein", Int Rev Cell Mol Biol, 267, pp.183~206 (2008)
- Steffen W, "Evidence for tektins in centrioles and axonemal microtubules", Proc Natl Acad Sci USA, 85, pp.2643~2647 (1988)
- Norrander JM, "Structural comparison of tektins and evidence for their determination of complex spacings in flagellar microtubules", J Mol Biol, 257, pp.385~397 (1996)
- Linck, RW, "Biochemical characterization of tektins from sperm flagellar doublet microtubules", J Cell Biol, 104, pp.1069~1075 (1987)
- Nojima D, "At least one of the protofilaments in flagellar microtubules is not composed of tubulin", Curr Biol, 5, pp.158~167 (1995)
- Zhang CX, "Science and technology in Chinese buffaloes" (2000), Guangxi Science and Technology Publishing House
- Park YJ, "Proteomic revolution to improve tools for evaluating male fertility in animals", J Proteome Res, 12, pp.4738~4747 (2013)
- Rahman MS, "Sperm proteomics: road to male fertility and contraception", Int J Endocrinol, 2013 (2013)
- Kwon WS, "Comparison of markers predicting litter size in different pig breeds", Andrology, 5, pp.568~577 (2017)
- Rahman MS, "Prediction of male fertility using capacitation-associated proteins in spermatozoa", Mol Reprod Dev, 84, pp.749~759 (2017)
- Yoon SJ, "Proteomic identification of cryostress in epididymal spermatozoa", J Anim Sci Biotechnol, 7, pp.67~ (2016)
- Kwon WS, "Discovery of predictive biomarkers for litter size in boar spermatozoa", Mol Cell Proteomics, 14, pp.1230~1240 (2015)
- Huang YL, "Differences between high‐and low‐motility Mmurrah buffalo sperm identified by comparative proteomics", Reprod Domest Anim, 50, pp.443~451 (2015)
- Kwon WS, "Increased male fertility using fertility-related biomarkers", Sci Rep, 5, pp.15654~ (2015)
- Kwon, WS, "Proteomic approaches for profiling negative fertility markers in inferior boar spermatozoa", Sci Rep, 5, pp.13821~ (2015)
- Park YJ, "Fertility-related proteomic profiling bull spermatozoa separated by percoll", J Proteome Res, 11, pp.4162~4168 (2012)
- Sharma A, "Vi polysaccharide of Salmonella typhi targets the prohibitin family of molecules in intestinal epithelial cells and suppresses early inflammatory responses", Proc Natl Acad Sci USA, 101, pp.17492~17497 (2004)
- Roskams AJ, "Cell cycle activity and expression of prohibitin mRNA", Cell Physiol, 157, pp.289~295 (1993)
- Piper PW, "The shortened replieative lifespan of prohibitin mutants of yeast appears to be due to defective mitochondrial segregation in old mother cells", Aging Cell, 1, pp.149~157 (2002)
- Bai SW, "Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration", BMC Biol, 9, pp.54~ (2011)
- Shimada K, "Aberrant expression of RAB1A in human tongue cancer", Br J Cancer, 92, pp.1915~1921 (2005)
- Vignjevic D, "Reorganisation of the dendritic actin network during cancer cell migration and invasion", Semin Cancer Biol, 18, pp.12~22 (2008)
- Carlier MF, "Control of actin dynamics in cell motility", J Mol Biol, 269, pp.459~467 (1997)
- Hopmann R, "Actin organization, bristle morphology, and viability are affected by actin capping protein mutations in Drosophila", J Cell Biol, 133, pp.1293~1305 (1996)
- Cooper JA, "New insights into mechanism and regulation of actin capping protein", Int Rev Cell Mol Biol, 267, pp.183~206 (2008)
- Tanaka H, "Mice deficient in the axonemal protein Tektin-t exhibit male infertility and immotile-cilium syndrome due to impaired inner arm dynein function", Mol Cell Biol, 24, pp.7958~7964 (2004)
- Shimasaki S, "Subcellular localization of Tektin2 in rat sperm flagellum", Zoolog Sci, 27, pp.755~761 (2010)
